CDC25B 抗体 (AA 119-248)
Quick Overview for CDC25B 抗体 (AA 119-248) (ABIN3043807)
抗原
See all CDC25B 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 119-248
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原理
- Anti-Cdc25B Antibody Picoband®
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交叉反应 （详细）
- No cross-reactivity with other proteins
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产品特性
- Anti-Cdc25B Antibody Picoband® (ABIN3043807). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human Cdc25B recombinant protein (Position: M119-L248). Human Cdc25B shares 71% and 68.2% amino acid (aa) sequence identity with mouse and rat Cdc25B, respectively.
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亚型
- IgG
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anti-Cell Division Cycle 25 Homolog B (S. Pombe) (CDC25B) (pSer186) antibody
CDC25B 适用: 小鼠 WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Cell Division Cycle 25 Homolog B (S. Pombe) (CDC25B) (pSer353) antibodyCDC25B 适用: 人, 小鼠 WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Cell Division Cycle 25 Homolog B (S. Pombe) (CDC25B) (pSer323) antibodyCDC25B 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Cell Division Cycle 25 Homolog B (S. Pombe) (CDC25B) (pSer149) antibodyCDC25B 适用: 人, 小鼠 WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Cell Division Cycle 25 Homolog B (S. Pombe) (CDC25B) (pSer323) antibodyCDC25B 适用: 小鼠 WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Cell Division Cycle 25 Homolog B (S. Pombe) (CDC25B) (AA 1-195) antibodyCDC25B 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Cell Division Cycle 25 Homolog B (S. Pombe) (CDC25B) (pSer353) antibodyCDC25B 适用: 人, 小鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Cell Division Cycle 25 Homolog B (S. Pombe) (CDC25B) (AA 331-360) antibodyCDC25B 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal RB07936 unconjugated
anti-Cell Division Cycle 25 Homolog B (S. Pombe) (CDC25B) (AA 1-580) antibodyCDC25B 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated
anti-Cell Division Cycle 25 Homolog B (S. Pombe) (CDC25B) (AA 56-105) antibodyCDC25B 适用: 人, 小鼠, 大鼠, 犬, 豚鼠, 马, Cow, Pig WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry(Fixed), 1-3 μg/1x106 cells, Human
1. Bulavin, D. V., Higashimoto, Y., Popoff, I. J., Gaarde, W. A., Basrur, V., Potapova, O., Appella, E., Fornace, A. J., Jr.Initiation of a G2/M checkpoint after ultraviolet radiation requires p38 kinase.Nature 411: 102-107, 2001. 2. Lane, S. A., Baker, E., Sutherland, G. R., Tonks, I., Hayward, N., Ellem, K.The human cell cycle gene CDC25B is located at 20p13.Genomics 15: 693-694, 1993. -
说明
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- CDC25B (Cell Division Cycle 25 Homolog B (S. Pombe) (CDC25B))
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别名
- CDC25B
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背景
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Synonyms: M-phase inducer phosphatase 2,3.1.3.48,Dual specificity phosphatase Cdc25B,CDC25B,CDC25HU2,
Tissue Specificity: Expressed on the plasma membranes of all cell types that are in intimate contact with plasma complement proteins. It is also found on the surfaces of epithelial cells lining extracellular compartments, and variants of the molecule are present in body fluids and in extracellular matrix.
Background: Central to the onset of mitosis in all eukaryotic cells is the CDC2 protein kinase, the activity of which is negatively regulated by phosphorylation and positively activated by dephosphorylation. The latter function is carried out by a specific phosphatase, CDC25. At least 3 human CDC25 genes code for the A, B, and C forms of CDC25. CDC25B is mapped to 20p13. P38 kinase has a critical role in the initiation of a G2 delay after ultraviolet radiation. Inhibition of p38 blocks the rapid initiation of this checkpoint in both human and murine cells after ultraviolet radiation. In vitro, p38 binds and phosphorylates CDC25B at serines 309 and 361, and CDC25C at serine-216, phosphorylation of these residues is required for binding to 14-3-3 proteins. In vivo, inhibition of p38 prevents both phosphorylation of CDC25B at serine-309 and 14-3-3 binding after ultraviolet radiation, and mutation of this site is sufficient to inhibit the checkpoint initiation. Regulation of CDC25B phosphorylation by p38 is a critical event for initiating the G2/M checkpoint after ultraviolet radiation.
Sequence Similarities: Belongs to the MPI phosphatase family.
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分子量
- 65 kDa
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UniProt
- P30305
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途径
- Cell Division Cycle, M Phase, Autophagy
抗原
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