我们推荐在您的浏览器上开启Javascript，以获得最佳效果。

CDK4 抗体 (AA 201-303)

Name: CDK4 抗体 (AA 201-303)
SKU: ABIN3043745
Availability: OutOfStock

CDK4 适用: 人, 小鼠, 大鼠 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated

Western Blotting (WB) image for anti-Cyclin-Dependent Kinase 4 (CDK4) (AA 201-303) antibody (ABIN3043745) (CDK4 抗体 (AA 201-303))

Anti- Cdk4 Picoband antibody, IHC(P) IHC(P): Human Lung Cancer Tissue (CDK4 抗体 (AA 201-303))

IHC

Anti- Cdk4 Picoband antibody, IHC(P) IHC(P): Mouse Intestine Tissue (CDK4 抗体 (AA 201-303))

IHC

Anti- Cdk4 Picoband antibody, IHC(P) IHC(P): Rat Intestine Tissue (CDK4 抗体 (AA 201-303))

IHC

4 images

(10 references)

产品编号 ABIN3043745

发货至: 中国

Datasheet as PDF

抗原 See all CDK4 抗体

CDK4 (Cyclin-Dependent Kinase 4 (CDK4))
抗原表位
16

15

14

9

9

8

6

5

4

3

3

2

2

2

2

2

2

1

1

1

1

1
AA 201-303
适用
115

65

50

18

7

5

4

4

3

1

1

1
人, 小鼠, 大鼠
宿主
99

35

2

1
兔
Compatible Secondaries
克隆类型
95

41
多克隆
标记
86

10

8

5

4

3

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This CDK4 antibody is un-conjugated
应用范围
114

49

33

31

24

23

22

12

12

9

6

3

2

1

1

1

1

1

1
Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

原理

Anti-Cdk4 Antibody Picoband®

交叉反应（详细）

No cross-reactivity with other proteins

产品特性

Anti-Cdk4 Antibody Picoband® (ABIN3043745). Tested in IF, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

纯化方法

Immunogen affinity purified.

免疫原

E.coli-derived human Cdk4 recombinant protein (Position: G201-E303). Human Cdk4 shares 94.2% amino acid (aa) sequence identity with both mouse and rat Cdk4.

亚型

IgG

Top Product

Discover our top product CDK4 Primary Antibody
anti-Cyclin-Dependent Kinase 4 (CDK4) antibody
KO Validated CDK4 适用: 人 WB, IF, ICC, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-Cyclin-Dependent Kinase 4 (CDK4) (N-Term) antibody
CDK4 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-Cyclin-Dependent Kinase 4 (CDK4) (AA 211-303) antibody
CDK4 适用: 人 WB, ELISA, IF, RNAi 宿主: 小鼠 Monoclonal 4F11 unconjugated

anti-Cyclin-Dependent Kinase 4 (CDK4) (AA 273-305), (C-Term) antibody
CDK4 适用: 人 WB, IF, FACS, IHC (p) 宿主: 兔 Polyclonal RB03154 unconjugated

anti-Cyclin-Dependent Kinase 4 (CDK4) (AA 6-295) antibody
CDK4 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Cyclin-Dependent Kinase 4 (CDK4) (AA 273-305) antibody
CDK4 适用: 人 WB, ELISA, IHC, IF, FACS 宿主: 兔 Polyclonal unconjugated

anti-Cyclin-Dependent Kinase 4 (CDK4) (AA 241-303) antibody
CDK4 适用: 人, 小鼠, 大鼠, 绵羊 WB, ELISA, FACS, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Cyclin-Dependent Kinase 4 (CDK4) (AA 272-303), (C-Term) antibody
CDK4 适用: 大鼠 WB, IF, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-Cyclin-Dependent Kinase 4 (CDK4) (AA 272-303) antibody
CDK4 适用: 人, 大鼠 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Cyclin-Dependent Kinase 4 (CDK4) (C-Term) antibody
CDK4 适用: 人, 大鼠, 绵羊, Pig, 犬, Cow, 兔, 豚鼠, 马 WB, IHC 宿主: 兔 Polyclonal unconjugated

应用备注

Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
Immunofluorescence, 5 μg/mL, Human
1. Demetrick, D. J., Zhang, H., Beach, D. H.: Chromosomal mapping of human CDK2, CDK4, and CDK5 cell cycle kinase genes. Cytogenet. Cell Genet. 66: 72-74, 1994. 2. Modiano, J. F., Mayor, J., Ball, C., Fuentes, M. K., Linthicum, D. S.: CDK4 expression and activity are required for cytokine responsiveness in T cells. J. Immun. 165: 6693-6702, 2000.e for human proliferating nuclear antigen/cyclin by in situ hybridization. Hum. Genet. 86: 84-86, 1990.

说明

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

限制

仅限研究用
状态

Lyophilized

溶解方式

Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

浓度

500 μg/mL

缓冲液

Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Avoid repeated freezing and thawing.

储存条件

4 °C,-20 °C

储存方法

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Ren, Huang, Xu, Yang, Yang, Hu: "Isoflavone lupiwighteone induces cytotoxic, apoptotic, and antiangiogenic activities in DU-145 prostate cancer cells." in: Anti-cancer drugs, Vol. 26, Issue 6, pp. 599-611, (2015) (PubMed).

Zhou, Lu, Liu, Guo, Liu, Zhou, Yang, Mi, Xu: "Platycodin D induces tumor growth arrest by activating FOXO3a expression in prostate cancer in vitro and in vivo." in: Current cancer drug targets, Vol. 14, Issue 9, pp. 860-71, (2015) (PubMed).

Zhang, Zhao, Huang, Meng, Bian, Li: "Melittin restores PTEN expression by down-regulating HDAC2 in human hepatocelluar carcinoma HepG2 cells." in: PLoS ONE, Vol. 9, Issue 5, pp. e95520, (2014) (PubMed).

Wang, Huang, Han, Xue, Li, Liu, Shan, Wang, Li, Li: "The effects of artemisinin on the proliferation and apoptosis of vascular smooth muscle cells of rats." in: Cell biochemistry and function, Vol. 32, Issue 2, pp. 201-8, (2014) (PubMed).

Li, Xu, Chu, Gao, Wang, Nie, Yang, Lv: "Molecular mechanism of inhibitory effects of CD59 gene on atherosclerosis in ApoE (-/-) mice." in: Immunology letters, Vol. 156, Issue 1-2, pp. 68-81, (2013) (PubMed).

Cai, Xu, Zhang: "Bone marrow stromal cells induce cell cycle arrest in reactive astrocytes in vitro." in: Neuroscience letters, Vol. 522, Issue 1, pp. 62-6, (2012) (PubMed).

Li, Xu, Li, Zou, Saimaiti, Simayi, Wang, Zhang, Yuan, Zhang: "Stat6 activity-related Th2 cytokine profile and tumor growth advantage of human colorectal cancer cells in vitro and in vivo." in: Cellular signalling, Vol. 24, Issue 3, pp. 718-25, (2012) (PubMed).

Zhang, Ge, Sun, Cao, Wu, Guo, Wang: "Effect of bone morphogenetic protein-2 on proliferation and apoptosis of gastric cancer cells." in: International journal of medical sciences, Vol. 9, Issue 2, pp. 184-92, (2012) (PubMed).

Liu, Dai, Lu, Wei, Ha, Rong, Mu, You, Li, Guo: "LYG-202 inhibits the proliferation of human colorectal carcinoma HCT-116 cells through induction of G1/S cell cycle arrest and apoptosis via p53 and p21(WAF1/Cip1) expression." in: Biochemistry and cell biology = Biochimie et biologie cellulaire, Vol. 89, Issue 3, pp. 287-98, (2011) (PubMed).

Han, Liu: "EGR-1 decoy ODNs inhibit vascular smooth muscle cell proliferation and neointimal hyperplasia of balloon-injured arteries in rat." in: Life sciences, Vol. 86, Issue 7-8, pp. 234-43, (2010) (PubMed).
抗原

CDK4 (Cyclin-Dependent Kinase 4 (CDK4))

别名

CDK4 (CDK4 产品)

背景

Synonyms: Cyclin-dependent kinase 4,2.7.11.22,Cell division protein kinase 4,PSK-J3,CDK4,
Tissue Specificity: Isoform 2 is found in breast cancer tissues.
Background: Cyclin-dependent kinase-4 (CDK4) is a protein-serine kinase involved in the cell cycle. Human cell division is regulated primarily at the G1-to-S or the G2-to-M boundaries within the cell cycle. The complexes formed by CDK4 and the D-type cyclins are involved in the control of cell proliferation during the G1 phase. CDK4 is inhibited by p16, also known as cyclin-dependent kinase inhibitor-2. CDK4 is mapped to 12q14. And CDK4 expression and activity are required for cytokine responsiveness in T cells.
Sequence Similarities: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.

分子量

34 kDa

基因ID

1019

UniProt

P11802

途径

Cell Division Cycle, Mitotic G1-G1/S Phases, Regulation of Cell Size