MAD2L1 抗体 (AA 2-205)
Quick Overview for MAD2L1 抗体 (AA 2-205) (ABIN3043608)
抗原
See all MAD2L1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 2-205
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原理
- Anti-Mad2L1 Antibody Picoband®
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交叉反应 （详细）
- No cross-reactivity with other proteins
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产品特性
- Anti-Mad2L1 Antibody Picoband® (ABIN3043608). Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human Mad2L1 recombinant protein (Position: A2-D205). Human Mad2L1 shares 94% amino acid (aa) sequence identity with mouse Mad2L1.
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亚型
- IgG
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anti-MAD2 Mitotic Arrest Deficient-Like 1 (Yeast) (MAD2L1) (C-Term) antibody
MAD2L1 适用: 人, 大鼠 WB, IF, IP, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated
anti-MAD2 Mitotic Arrest Deficient-Like 1 (Yeast) (MAD2L1) (AA 1-205) antibodyMAD2L1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 2E2-1D6 unconjugated
anti-MAD2 Mitotic Arrest Deficient-Like 1 (Yeast) (MAD2L1) (AA 1-205) antibodyMAD2L1 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-MAD2 Mitotic Arrest Deficient-Like 1 (Yeast) (MAD2L1) (AA 1-174) antibodyMAD2L1 适用: 人 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-MAD2 Mitotic Arrest Deficient-Like 1 (Yeast) (MAD2L1) antibodyMAD2L1 适用: 人 WB, IP, IHC (p) 宿主: 小鼠 Monoclonal 17D10 unconjugated
anti-MAD2 Mitotic Arrest Deficient-Like 1 (Yeast) (MAD2L1) (Internal Region) antibodyMAD2L1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-MAD2 Mitotic Arrest Deficient-Like 1 (Yeast) (MAD2L1) (AA 131-205) antibodyMAD2L1 适用: 人 ELISA, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-MAD2 Mitotic Arrest Deficient-Like 1 (Yeast) (MAD2L1) (AA 2-205) antibodyMAD2L1 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-MAD2 Mitotic Arrest Deficient-Like 1 (Yeast) (MAD2L1) (AA 1-205) antibodyMAD2L1 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-MAD2 Mitotic Arrest Deficient-Like 1 (Yeast) (MAD2L1) antibodyMAD2L1 适用: 人, 小鼠, 大鼠 IHC, IF 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.1-0.5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
1. Cahill, D. P., da Costa, L. T., Carson-Walter, E. B., Kinzler, K. W., Vogelstein, B., Lengauer, C.Characterization of MAD2B and other mitotic spindle checkpoint genes. Genomics 58: 181-187, 1999. 2. "Entrez Gene: MAD2L1 MAD2 mitotic arrest deficient-like 1 (yeast)" -
说明
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- MAD2L1 (MAD2 Mitotic Arrest Deficient-Like 1 (Yeast) (MAD2L1))
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别名
- MAD2L1
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背景
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Synonyms: Mitotic spindle assembly checkpoint protein MAD2A,HsMAD2,Mitotic arrest deficient 2-like protein 1,MAD2-like protein 1,MAD2L1,MAD2,
Tissue Specificity: Expressed in the brain. Expressed in pyramidal neurons in all cortical laminae of the visual cortex, in neurons of the substantia nigra pars compacta and caudate putamen (at protein level). Expressed throughout the adult brain, but at a lower level than in heart and liver. Also expressed in placenta, lung, skeletal muscle, kidney and pancreas. In the brain, expressed in the cerebellum, cerebral cortex, medulla, spinal cord occipital pole, frontal lobe, temporal lobe and putamen. Expression is particularly high in brain dopaminoceptive areas. .
Background: Mitotic spindle assembly checkpoint protein MAD2A is a protein that in humans is encoded by the MAD2L1 gene. This gene belongs to the MAD2 family. It is mapped to 4q27. MAD2L1 is a component of the mitotic spindle assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. The protein has two highly different native conformations, an inactive open conformation that cannot bind CDC20 and that predominates in cytosolic monomers, and an active closed conformation. It is required for the execution of the mitotic checkpoint which monitors the process of kinetochore-spindle attachment.
Sequence Similarities: Belongs to the MAD2 family.
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分子量
- 23 kDa
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基因ID
- 4085
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UniProt
- Q13257
抗原
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