PGK1 抗体 (C-Term)
Quick Overview for PGK1 抗体 (C-Term) (ABIN3043604)
抗原
See all PGK1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 312-337, C-Term
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原理
- Anti-PGK1 Antibody Picoband®
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序列
- MGLDCGPESS KKYAEAVTRA KQIVWN
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-PGK1 Antibody Picoband® (ABIN3043604). Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- A synthetic peptide corresponding to a sequence at the C-terminus of human PGK1, different from the related mouse and rat sequences by two amino acids.
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亚型
- IgG
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anti-Phosphoglycerate Kinase 1 (PGK1) (AA 2-417) antibody
PGK1 适用: 人 WB, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Phosphoglycerate Kinase 1 (PGK1) (AA 1-417) antibodyPGK1 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Phosphoglycerate Kinase 1 (PGK1) (AA 117-145) antibodyPGK1 适用: 人 WB, IF, IHC (p), FACS 宿主: 兔 Polyclonal RB05463 unconjugated
anti-Phosphoglycerate Kinase 1 (PGK1) (AA 117-145) antibodyPGK1 适用: 人, 小鼠 WB, ELISA, IHC, IF, FACS 宿主: 兔 Polyclonal unconjugated
anti-Phosphoglycerate Kinase 1 (PGK1) antibodyPGK1 适用: 人, 小鼠, 大鼠 IHC 宿主: 兔 Polyclonal unconjugated
anti-Phosphoglycerate Kinase 1 (PGK1) (AA 1-417) antibodyPGK1 适用: 人, 小鼠 WB, IHC (p), FACS 宿主: 小鼠 Monoclonal 1086CT10-2-1 unconjugated
anti-Phosphoglycerate Kinase 1 (PGK1) antibodyPGK1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Phosphoglycerate Kinase 1 (PGK1) (AA 321-417) antibodyPGK1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 2H4 unconjugated
anti-Phosphoglycerate Kinase 1 (PGK1) antibodyPGK1 适用: 人, 小鼠 WB, IHC (p), FACS 宿主: 小鼠 Monoclonal 6C1 unconjugated
anti-Phosphoglycerate Kinase 1 (PGK1) (AA 321-417) antibodyPGK1 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
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应用备注
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Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
1. "Entrez Gene: PGK1 phosphoglycerate kinase 1". 2. Michelson AM, Markham AF, Orkin SH (May 1983). "Isolation and DNA sequence of a full-length cDNA clone for human X chromosome-encoded phosphoglycerate kinase". Proc Natl Acad Sci U S A 80 (2): 472-6. 3. Singer-Sam J, Keith DH, Tani K, Simmer RL, Shively L, Lindsay S, Yoshida A, Riggs AD (May 1985). "Sequence of the promoter region of the gene for human X-linked 3-phosphoglycerate kinase]". Gene 32 (3): 409-17. -
说明
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- PGK1 (Phosphoglycerate Kinase 1 (PGK1))
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别名
- PGK1
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背景
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Synonyms: Phosphoglycerate kinase 1,2.7.2.3,Cell migration-inducing gene 10 protein,Primer recognition protein 2,PRP 2,PGK1,PGKA,MIG10, OK/SW-cl.110,
Tissue Specificity: Ubiquitous, highest levels of expression in heart and skeletal muscle. .
Background: PGK1 (Phosphoglycerate Kinase 1), also known as PGKA, is an enzyme that in humans is encoded by the PGK1 gene. The protein encoded by this gene is a glycolytic enzyme that catalyzes the conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate. The encoded protein may also act as a cofactor for polymerase alpha. Additionally, this protein is secreted by tumor cells where it participates in angiogenesis by functioning to reduce disulfide bonds in the serine protease, plasmin, which consequently leads to the release of the tumor blood vessel inhibitor angiostatin. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Deficiency of the enzyme is associated with a wide range of clinical phenotypes hemolytic anemia and neurological impairment. Pseudogenes of this gene have been defined on chromosomes 19, 21 and the X chromosome.
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分子量
- 43 kDa
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基因ID
- 5230
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UniProt
- P00558
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途径
- Cellular Glucan Metabolic Process
抗原
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