c-MYC 抗体 (AA 257-439)
(13 references)Quick Overview for c-MYC 抗体 (AA 257-439) (ABIN3043598)
抗原
See all c-MYC (MYC) 抗体适用
宿主
克隆类型
标记
应用范围
-
-
抗原表位
- AA 257-439
-
原理
- Anti-c-Myc Antibody Picoband®
-
交叉反应 （详细）
- No cross-reactivity with other proteins
-
产品特性
- Anti-c-Myc Antibody Picoband® (ABIN3043598). Tested in IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
-
纯化方法
- Immunogen affinity purified.
-
免疫原
- E.coli-derived human c-Myc recombinant protein (Position: E257-A439). Human c-Myc shares 91% amino acid (aa) sequences identity with both mouse and rat c-Myc.
-
亚型
- IgG
-
-
anti-Myc Proto-Oncogene protein (MYC) (AA 330-439) antibody
MYC 适用: 人 WB, ELISA, IF, IHC (p) 宿主: 小鼠 Monoclonal 1G7 unconjugated
anti-Myc Proto-Oncogene protein (MYC) (Center) antibodyMYC 适用: 人, 小鼠 WB, IF, IP, ICC, ChIP 宿主: 兔 Polyclonal unconjugated
anti-Myc Proto-Oncogene protein (MYC) (AA 31-80) antibodyMYC 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Myc Proto-Oncogene protein (MYC) (pSer62) antibodyMYC 适用: 人, 小鼠, 大鼠 IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Myc Proto-Oncogene protein (MYC) (pThr58) antibodyMYC 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Myc Proto-Oncogene protein (MYC) (pSer62) antibodyMYC 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Myc Proto-Oncogene protein (MYC) antibodyKO Validated MYC 适用: 人 WB, IF, ChIP 宿主: 兔 Monoclonal unconjugated
anti-Myc Proto-Oncogene protein (MYC) (AA 101-200) antibodyMYC 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p), ICC, IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Myc Proto-Oncogene protein (MYC) (N-Term) antibodyMYC 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Myc Proto-Oncogene protein (MYC) antibodyMYC 适用: 人 ELISA 宿主: 小鼠 Monoclonal 7E10 unconjugated
-
-
应用备注
-
Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
1. Liao D, Thakur A, Wu J, Biliran H, Sarkar FH. Perspectives on c-Myc, Cyclin D1, and Their Interaction in Cancer Formation, Progression, and Response to Chemotherapy.Crit Rev Oncog. 2007,13(2):93-158. 2. Britton S, Salles B, Calsou P. c-MYC protein is degraded in response to UV irradiation.Cell Cycle. 2007 Oct 2,7(1) 3. Chow JM, Liu CR, Lin CP, Lee CN, Cheng YC, Lin S, Liu HE. Downregulation of c-Myc determines sensitivity to 2-methoxyestradiol-induced apoptosis in human acute myeloid leukemia.Exp Hematol. 2008 Feb,36(2):140-8. 4. Huang CY, Bredemeyer AL, Walker LM, Bassing CH, Sleckman BP. Dynamic regulation of c-Myc proto-oncogene expression during lymphocyte development revealed by a GFP-c-Myc knock-in mouse.Eur J Immunol. 2008 Jan 14. -
说明
-
Antibody can be supported by chemiluminescence kit ABIN921124 in WB.
-
限制
- 仅限研究用
-
-
-
状态
- Lyophilized
-
溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
-
浓度
- 500 μg/mL
-
缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
-
注意事项
- Avoid repeated freezing and thawing.
-
储存条件
- 4 °C,-20 °C
-
储存方法
-
Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
-
-
-
: "The novel protein C9orf116 promotes rat liver cell line BRL-3A proliferation." in: PLoS ONE, Vol. 12, Issue 7, pp. e0180607, (2017) (PubMed).
: "The novel protein C3orf43 accelerates hepatocyte proliferation." in: Cellular & molecular biology letters, Vol. 22, pp. 21, (2017) (PubMed).
: "LiCl inhibits PRRSV infection by enhancing Wnt/?-catenin pathway and suppressing inflammatory responses." in: Antiviral research, Vol. 117, pp. 99-109, (2015) (PubMed).
: "Design, synthesis and biological evaluation of N-alkyl or aryl substituted isoindigo derivatives as potential dual cyclin-dependent kinase 2 (CDK2)/glycogen synthase kinase 3? (GSK-3?) ..." in: European journal of medicinal chemistry, Vol. 86, pp. 165-74, (2014) (PubMed).
: "Litopenaeus vannamei sterile-alpha and armadillo motif containing protein (LvSARM) is involved in regulation of Penaeidins and antilipopolysaccharide factors." in: PLoS ONE, Vol. 8, Issue 2, pp. e52088, (2013) (PubMed).
: "Down-regulation of miR-129-5p inhibits growth and induces apoptosis in laryngeal squamous cell carcinoma by targeting APC." in: PLoS ONE, Vol. 8, Issue 10, pp. e77829, (2013) (PubMed).
: "Leptin induces cell proliferation and reduces cell apoptosis by activating c-myc in cervical cancer." in: Oncology reports, Vol. 29, Issue 6, pp. 2291-6, (2013) (PubMed).
: "HILI inhibits TGF-? signaling by interacting with Hsp90 and promoting T?R degradation." in: PLoS ONE, Vol. 7, Issue 7, pp. e41973, (2012) (PubMed).
: "RPB5-mediating protein is required for the proliferation of hepatocellular carcinoma cells." in: The Journal of biological chemistry, Vol. 286, Issue 13, pp. 11865-74, (2011) (PubMed).
: "Effects of matrine on HepG2 cell proliferation and expression of tumor relevant proteins in vitro." in: Pharmaceutical biology, Vol. 48, Issue 3, pp. 275-81, (2010) (PubMed).
: "Osteogenic role of endosomal chloride channels in MC3T3-E1 cells." in: Molecular and cellular biochemistry, Vol. 342, Issue 1-2, pp. 191-9, (2010) (PubMed).
: "Molecular cloning, expression and subcellular distribution of an alternative splice variant of the porcine Sirt2 gene." in: Molecular biology reports, Vol. 37, Issue 3, pp. 1671-6, (2010) (PubMed).
: "Let-7a microRNA functions as a potential tumor suppressor in human laryngeal cancer." in: Oncology reports, Vol. 22, Issue 5, pp. 1189-95, (2009) (PubMed).
-
: "The novel protein C9orf116 promotes rat liver cell line BRL-3A proliferation." in: PLoS ONE, Vol. 12, Issue 7, pp. e0180607, (2017) (PubMed).
-
- c-MYC (MYC) (Myc Proto-Oncogene protein (MYC))
-
别名
- MYC
-
背景
-
Synonyms: Myc proto-oncogene protein,Class E basic helix-loop-helix protein 39,bHLHe39,Proto-oncogene c-Myc,Transcription factor p64,MYC,BHLHE39,
Tissue Specificity: Expressed in all fetal tissues examined with highest levels in brain, kidney, heart and spleen. Weak expression in liver. In adult brain, highest expression found in the frontal lobe of the cortex and in the anterior perisylvian cortex- opercular gyri. Moderate expression in the cerebellar cortex, the posterior perisylvian cortex-opercular gyri and the temporal associated cortex. Weak expression found in the striate, extra- striate and motor cortices. Expressed in cerebrospinal fluid, and plasma. Isoform APP695 is the predominant form in neuronal tissue, isoform APP751 and isoform APP770 are widely expressed in non- neuronal cells. Isoform APP751 is the most abundant form in T- lymphocytes. Appican is expressed in astrocytes. .
Background: C-Myc is an oncogene that functions both in the stimulation of cell proliferation and in apoptosis. C-Myc elicits its oncogenic activity by causing immortalization, and to a lesser extent the transformation of cells, in addition to several other mechanisms. The c-MYC proto-oncogene encodes a transcription factor that is critical for cell growth and proliferation. It is one of the genes frequently altered in cancer cells in which it exhibits constitutive activity. Downregulation of c-Myc is critical for 2-Methoxyestradiol (2ME2)-induced oxidative stress and apoptosis in AML cells. And its up-regulation is important for promoting lymphocyte cell division, and demonstrating that GFP-c-Myc expression is a marker of proliferating lymphocytes in vivo.
Sequence Similarities: Contains 1 bHLH (basic helix-loop-helix) domain.
-
分子量
- 60-65 kDa
-
基因ID
- 4609
-
UniProt
- P01106
-
途径
- p53 Pathway, Cell Division Cycle, Sensory Perception of Sound, Transition Metal Ion Homeostasis, Mitotic G1-G1/S Phases, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Positive Regulation of Response to DNA Damage Stimulus, Warburg Effect
抗原
-
