TdT 抗体 (AA 316-509)
(9 references)Quick Overview for TdT 抗体 (AA 316-509) (ABIN3043577)
抗原
See all TdT (DNTT) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 316-509
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原理
- Anti-TdT/DNTT Antibody Picoband®
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交叉反应 （详细）
- No cross-reactivity with other proteins
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产品特性
- Anti-TdT/DNTT Antibody Picoband® (ABIN3043577). Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human TdT recombinant protein (Position: K316-A509). Human TdT shares 81% amino acid (aa) sequence identity with mouse TdT.
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亚型
- IgG
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anti-Deoxynucleotidyltransferase, terminal (DNTT) (AA 52-192) antibody
DNTT 适用: 人, 大鼠 WB, ELISA, FACS 宿主: 小鼠 Monoclonal 4B10A6 unconjugated
anti-Deoxynucleotidyltransferase, terminal (DNTT) (AA 52-192) antibodyDNTT 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 4B10C3 unconjugated
anti-Deoxynucleotidyltransferase, terminal (DNTT) (AA 1-110) antibodyDNTT 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 4H5 unconjugated
anti-Deoxynucleotidyltransferase, terminal (DNTT) antibodyDNTT 适用: 人 IHC 宿主: 兔 Monoclonal MSVA-453R unconjugated Recombinant Antibody
anti-Deoxynucleotidyltransferase, terminal (DNTT) (AA 351-450) antibodyDNTT 适用: 人, 小鼠, 大鼠 ELISA, FACS, IHC (p), IHC (fro), IF (cc), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-Deoxynucleotidyltransferase, terminal (DNTT) (AA 1-509) antibodyDNTT 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Deoxynucleotidyltransferase, terminal (DNTT) (Internal Region) antibodyDNTT 适用: 人 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Deoxynucleotidyltransferase, terminal (DNTT) (AA 32-81) antibodyDNTT 适用: 人, 小鼠, 大鼠, 马, Cow, 犬, Pig, 兔, Bat WB 宿主: 兔 Polyclonal unconjugated
anti-Deoxynucleotidyltransferase, terminal (DNTT) antibodyDNTT 适用: 人, 小鼠, 大鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Deoxynucleotidyltransferase, terminal (DNTT) (C-Term) antibodyDNTT 适用: 人, 大鼠, 猴, 马, 犬, 豚鼠, Pig, Bat WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.1-0.5 μg/mL, Human
1. Landau, N. R., St. John, T. P., Weissman, I. L., Wolf, S. C., Silverstone, A. E., Baltimore, D. : Cloning of terminal transferase cDNA by antibody screening. Proc. Nat. Acad. Sci. 81: 5836-5840, 1984. 2. Yang-Feng, T. L., Landau, N. R., Baltimore, D., Francke, U.: The terminal deoxynucleotidyltransferase gene is located on human chromosome 10 (10q23-q24) and on mouse chromosome 19. Cytogenet. Cell Genet. 43: 121-126, 1986. 3. Riley, L. K., Morrow, J. K., Danton, M. J., Coleman, M. S.: Human terminal deoxyribonucleotidyltransferase: molecular cloning and structural analysis of the gene and 5-prime flanking region. Proc. Nat. Acad. Sci. 85: 2489-2493, 1988. -
说明
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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: "Antitumour activity of the recombination polypeptide GST-NT21MP is mediated by inhibition of CXCR4 pathway in breast cancer." in: British journal of cancer, Vol. 110, Issue 5, pp. 1288-97, (2014) (PubMed).
: "Bone morphogenetic protein 7 upregulates the expression of nestin and glial fibrillary acidic protein in rats with cerebral ischemia-reperfusion injury." in: Biomedical reports, Vol. 1, Issue 6, pp. 895-900, (2014) (PubMed).
: "Histopathological studies of microtubule disassembling agent-induced myocardial lesions in rats." in: Experimental and toxicologic pathology : official journal of the Gesellschaft fu?r Toxikologische Pathologie, Vol. 65, Issue 6, pp. 737-43, (2013) (PubMed).
: "Annexin A3 is associated with a poor prognosis in breast cancer and participates in the modulation of apoptosis in vitro by affecting the Bcl-2/Bax balance." in: Experimental and molecular pathology, Vol. 95, Issue 1, pp. 23-31, (2013) (PubMed).
: "Combined adenovirus-mediated artificial microRNAs targeting mfgl2, mFas, and mTNFR1 protect against fulminant hepatic failure in mice." in: PLoS ONE, Vol. 8, Issue 11, pp. e82330, (2013) (PubMed).
: "Coexistence of hyperlipidemia and acute cerebral ischemia/reperfusion induces severe liver damage in a rat model." in: World journal of gastroenterology, Vol. 18, Issue 35, pp. 4934-43, (2012) (PubMed).
: "A small dose of hydrogen peroxide enhances tumor necrosis factor-alpha toxicity in inducing human vascular endothelial cell apoptosis: reversal with propofol." in: Anesthesia and analgesia, Vol. 103, Issue 1, pp. 110-6, table of contents, (2006) (PubMed).
: "Propofol dose-dependently reduces tumor necrosis factor-alpha-Induced human umbilical vein endothelial cell apoptosis: effects on Bcl-2 and Bax expression and nitric oxide generation." in: Anesthesia and analgesia, Vol. 100, Issue 6, pp. 1653-9, (2005) (PubMed).
: "Possible mechanisms of the protection of ginsenoside Re against MPTP-induced apoptosis in substantia nigra neurons of Parkinson's disease mouse model." in: Journal of Asian natural products research, Vol. 7, Issue 3, pp. 215-24, (2004) (PubMed).
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: "Antitumour activity of the recombination polypeptide GST-NT21MP is mediated by inhibition of CXCR4 pathway in breast cancer." in: British journal of cancer, Vol. 110, Issue 5, pp. 1288-97, (2014) (PubMed).
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- TdT (DNTT) (Deoxynucleotidyltransferase, terminal (DNTT))
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别名
- DNTT
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背景
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Synonyms: DNA nucleotidylexotransferase,2.7.7.31 ,Terminal addition enzyme,Terminal deoxynucleotidyltransferase,Terminal transferase ,DNTT,TDT ,
Tissue Specificity: Widely expressed. .
Background: Terminal Deoxynucleotidyl Transferase, also known as TDT and terminal transferase, is a unique DNA polymerase without template ion catalyzes the addition of deoxyribonucleotides onto the 3-prime-hydroxyl end of DNA primers. Its gene is mapped to the region 10q23-q24. And TDT cDNA contains an open reading frame of 1,530 basepairs corresponding to a protein containing 510 amino acids. TDT may be responsible for inserting nucleotides (N regions) at the V (H)-D and D-J (H) junctions of immunoglobulin genes. The enzyme is present in immature thymocytes, some bone marrow cells, transformed pre-B and pre-T cell lines, and leukemia cells. Additionally, TDT catalyses the addition of nucleotides to the 3' terminus of a DNA molecule. Unlike most DNA polymerases it does not require a template. The preferred substrate of this enzyme is a 3'-overhang, but it can also add nucleotides to blunt or recessed 3' ends. Cobalt is a necessary cofactor.
Sequence Similarities: Belongs to the DNA polymerase type-X family.
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分子量
- 58 kDa
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基因ID
- 1791
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UniProt
- P04053
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途径
- DNA Damage Repair
抗原
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