Perforin 1 抗体 (AA 175-555)
Quick Overview for Perforin 1 抗体 (AA 175-555) (ABIN3043547)
抗原
See all Perforin 1 (PRF1) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 175-555
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原理
- Anti-Perforin/PRF1 Antibody
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交叉反应 （详细）
- No cross-reactivity with other proteins
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产品特性
- Anti-Perforin/PRF1 Antibody (ABIN3043547). Tested in WB applications. This antibody reacts with Human. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human Perforin recombinant protein (Position: E175-W555). Human Perforin shares 68% amino acid (aa) sequence identity with both mouse and rat Perforin.
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亚型
- IgG
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anti-Perforin 1 (Pore Forming Protein) (PRF1) (AA 280-555) antibody
PRF1 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Perforin 1 (Pore Forming Protein) (PRF1) antibodyPRF1 适用: 人, 小鼠, 大鼠 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Perforin 1 (Pore Forming Protein) (PRF1) (AA 461-555) antibodyPRF1 适用: 人 WB, ELISA, IP 宿主: 小鼠 Monoclonal 3B4 unconjugated
anti-Perforin 1 (Pore Forming Protein) (PRF1) antibodyPRF1 适用: 人, Cow IP, FACS, ICC, IHC (fro), IHC (p) 宿主: 小鼠 Monoclonal dG9 unconjugated
anti-Perforin 1 (Pore Forming Protein) (PRF1) (AA 40-355) antibodyPRF1 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Perforin 1 (Pore Forming Protein) (PRF1) (AA 413-552) antibodyPRF1 适用: 人 IHC, ELISA, Coat, StM 宿主: 小鼠 Monoclonal PRF1-2470 unconjugated
anti-Perforin 1 (Pore Forming Protein) (PRF1) antibody (FITC)PRF1 适用: 人, Cow ICFC 宿主: 小鼠 Monoclonal dG9 FITC
anti-Perforin 1 (Pore Forming Protein) (PRF1) antibody (APC)PRF1 适用: 人, Cow ICFC 宿主: 小鼠 Monoclonal dG9 APC
anti-Perforin 1 (Pore Forming Protein) (PRF1) (AA 32-316) antibodyPRF1 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Perforin 1 (Pore Forming Protein) (PRF1) (AA 21-554) antibodyPRF1 适用: 小鼠 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.1-0.5 μg/mL, Human
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说明
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- Perforin 1 (PRF1) (Perforin 1 (Pore Forming Protein) (PRF1))
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别名
- PRF1
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背景
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Synonyms: Perforin-1,P1,Cytolysin,Lymphocyte pore-forming protein,PFP,PRF1,PFP,
Tissue Specificity: Highly expressed in the heart, skeletal muscle, placenta and lung. Weakly expressed in the thymus, spleen, kidney and liver. Expressed on activated T- and B-cells, dendritic cells, keratinocytes and monocytes. .
Background: PRF1, also known as Perforin-1, is a protein that in humans is encoded by the PRF1 gene. It is mapped to 10q22.1. PRF1 is a cytolytic protein found in the granules of Cytotoxic T lymphocytes (CTLs) and NK cells. Upon degranulation, PRF1 inserts itself into the target cell's plasma membrane, forming a pore. The lytic membrane-inserting part of perforin is the MACPF domain. This region shares homology with cholesterol-dependent cytolysins from Gram-positive bacteria. PRF1 has structural and functional similarities to complement component 9 (C9). Like C9, this protein creates transmembrane tubules and is capable of lysing non-specifically a variety of target cells. This protein is one of the main cytolytic proteins of cytolytic granules, and it is known to be a key effector molecule for T-cell- and natural killer-cell-mediated cytolysis. PRF1 is thought to act by creating holes in the plasma membrane which triggers an influx of calcium and initiates membrane repair mechanisms. These repair mechanisms bring perforin and granzymes into early endosomes.
Sequence Similarities: Belongs to the complement C6/C7/C8/C9 family.
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分子量
- 61 kDa
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基因ID
- 5551
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UniProt
- P14222
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途径
- Apoptosis, Caspase Cascade in Apoptosis
抗原
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