NUP98 抗体 (AA 549-880)
Quick Overview for NUP98 抗体 (AA 549-880) (ABIN3043456)
抗原
See all NUP98 抗体适用
宿主
克隆类型
标记
应用范围
-
-
抗原表位
- AA 549-880
-
原理
- Anti-NUP98 Antibody Picoband®
-
交叉反应 （详细）
- No cross-reactivity with other proteins
-
产品特性
- Anti-NUP98 Antibody Picoband® (ABIN3043456). Tested in WB applications. This antibody reacts with Human, Monkey, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
-
纯化方法
- Immunogen affinity purified.
-
免疫原
- E.coli-derived human NUP98 recombinant protein (Position: H549-F880). Human NUP98 shares 95% and 94% amino acid (aa) sequence identity with mouse and rat NUP98, respectively.
-
亚型
- IgG
-
-
anti-Nucleoporin 98kDa (NUP98) (AA 1-466) antibody
NUP98 适用: 人, 小鼠, 大鼠, Saccharomyces cerevisiae, Schizosaccharomyces pombe WB, ELISA, IF, ICC, DB 宿主: 大鼠 Monoclonal 2H10 unconjugated
anti-Nucleoporin 98kDa (NUP98) (GLFG Motif), (N-Term) antibodyNUP98 适用: 人, 小鼠, Saccharomyces cerevisiae, Schizosaccharomyces pombe, Tetrahymena WB, IF 宿主: 小鼠 Monoclonal 21A10 unconjugated
anti-Nucleoporin 98kDa (NUP98) (FGxxN Motif), (N-Term) antibodyNUP98 适用: 人, Saccharomyces cerevisiae, Schizosaccharomyces pombe, Tetrahymena WB, IF 宿主: 小鼠 Monoclonal 13C2 unconjugated
anti-Nucleoporin 98kDa (NUP98) (AA 965-1110) antibodyNUP98 适用: 人 WB, ELISA, IP, IF 宿主: 兔 Polyclonal unconjugated
anti-Nucleoporin 98kDa (NUP98) antibodyNUP98 适用: 人 WB, ELISA, IF, IHC 宿主: 兔 Monoclonal 2H4 unconjugated Recombinant Antibody
anti-Nucleoporin 98kDa (NUP98) (AA 621-920) antibodyNUP98 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Nucleoporin 98kDa (NUP98) antibodyNUP98 适用: 人 WB, IP 宿主: 小鼠 Monoclonal unconjugated
anti-Nucleoporin 98kDa (NUP98) (AA 1-606) antibodyNUP98 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-Nucleoporin 98kDa (NUP98) antibodyNUP98 适用: 人, 小鼠, 大鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Nucleoporin 98kDa (NUP98) (AA 1-110) antibodyNUP98 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
-
-
应用备注
-
Western blot, 0.1-0.5 μg/mL, Human, Monkey, Mouse, Rat
1. Laurell, E., Beck, K., Krupina, K., Theerthagiri, G., Bodenmiller, B., Horvath, P., Aebersold, R., Antonin, W., Kutay, U. Phosphorylation of Nup98 by multiple kinases is crucial for NPC disassembly during mitotic entry. Cell 144: 539-550, 2011. 2. Nakamura, T., Largaespada, D. A., Lee, M. P., Johnson, L. A., Ohyashiki, K., Toyama, K., Chen, S. J., Willman, C. L., Chen, I.-M., Feinberg, A. P., Jenkins, N. A., Copeland, N. G., Shaughnessy, J. D., Jr. Fusion of the nucleoporin gene NUP98 to HOXA9 by the chromosome translocation t(7,11)(p15,p15) in human myeloid leukaemia. Nature Genet. 12: 154-158, 1996. 3. von Kobbe, C., van Deursen, J. M. A., Rodrigues, J. P., Sitterlin, D., Bachi, A., Wu, X., Wilm, M., Carmo-Fonseca, M., Izaurralde, E. Vesicular stomatitis virus matrix protein inhibits host cell gene expression by targeting the nucleoporin Nup98. Molec. Cell 6: 1243-1252, 2000. -
说明
-
Antibody can be supported by chemiluminescence kit ABIN921124 in WB.
-
限制
- 仅限研究用
-
-
-
状态
- Lyophilized
-
溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
-
浓度
- 500 μg/mL
-
缓冲液
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.01 mg Sodium azide.
-
储存液
- Sodium azide
-
注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
注意事项
- Avoid repeated freezing and thawing.
-
储存条件
- 4 °C,-20 °C
-
储存方法
-
Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
-
-
- NUP98 (Nucleoporin 98kDa (NUP98))
-
别名
- NUP98
-
背景
-
Synonyms: Nuclear pore complex protein Nup98-Nup96,Nuclear pore complex protein Nup98,98 kDa nucleoporin,Nucleoporin Nup98,Nup98,Nuclear pore complex protein Nup96,96 kDa nucleoporin,Nucleoporin Nup96,Nup96,NUP98,ADAR2,
Tissue Specificity: The expression of isoforms 1 and 2 does not seem to overlap. Isoform 1 is expressed by the blood vessels of different tissues. In the developing embryo it is found predominantly in the nervous system. In adult tissues, it is highly expressed in heart and placenta, moderately in lung, liver, skeletal muscle, kidney and pancreas, and low in adult brain. Isoform 2 is found in liver hepatocytes, kidney distal and proximal tubules.
Background: Nuclear pore complex protein Nup98-Nup96 is a protein that in humans is encoded by the NUP98 gene. This gene is one of several genes located in the imprinted gene domain of 11p15.5, an important tumor-suppressor gene region. NUP98 is a peripheral nucleoporin located at both the cytoplasmic and nuclear sides of the central channel of the NPC. NUP98 phosphorylation is critical for NPC disassembly at the onset of mitosis. It also plays roles in gene expression, mitotic checkpoint, and pathogenesis. Ligand blot analysis suggested that NUP98 can function as a docking protein for cytosol-mediated docking of import substrates. In addition to that, NUP98 is a target of the vesicular stomatitis virus M protein-mediated inhibition of mRNA nuclear export.
Sequence Similarities: Belongs to the nucleoporin GLFG family.
-
分子量
- 98 kDa
-
基因ID
- 4928
-
UniProt
- P52948
-
途径
- Stem Cell Maintenance, Protein targeting to Nucleus, SARS-CoV-2 Protein Interactome
抗原
-
