MSK1 抗体 (AA 540-665)
Quick Overview for MSK1 抗体 (AA 540-665) (ABIN3043424)
抗原
See all MSK1 (RPS6KA5) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 540-665
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原理
- Anti-MSK1/RPS6KA5 Antibody Picoband®
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交叉反应 （详细）
- No cross-reactivity with other proteins
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产品特性
- Anti-MSK1/RPS6KA5 Antibody Picoband® (ABIN3043424). Tested in IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human MSK1 recombinant protein (Position: V540-Q665). Human MSK1 shares 98% amino acid (aa) sequence identity with mouse MSK1.
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亚型
- IgG
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anti-Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 5 (RPS6KA5) (AA 224-367) antibody
RPS6KA5 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 5 (RPS6KA5) (AA 271-370) antibodyRPS6KA5 适用: 人 WB, ELISA, IF, PLA 宿主: 小鼠 Monoclonal 2B11 unconjugated
anti-Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 5 (RPS6KA5) (pSer376) antibodyRPS6KA5 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 5 (RPS6KA5) antibodyRPS6KA5 适用: 人 ELISA, IHC, IHC (p) 宿主: 小鼠 Monoclonal 1G7 unconjugated
anti-Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 5 (RPS6KA5) (AA 1-802) antibodyRPS6KA5 适用: 人 WB, PLA 宿主: 兔 Polyclonal unconjugated
anti-Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 5 (RPS6KA5) (Ser212) antibodyRPS6KA5 适用: 人, 小鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 5 (RPS6KA5) (Internal Region) antibodyRPS6KA5 适用: 人, 小鼠 WB, IF, IHC (p), EIA 宿主: 兔 Polyclonal unconjugated
anti-Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 5 (RPS6KA5) (pSer212) antibodyRPS6KA5 适用: 大鼠 WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 5 (RPS6KA5) (pSer360) antibodyRPS6KA5 适用: 小鼠, 大鼠 WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 5 (RPS6KA5) (pSer376) antibodyRPS6KA5 适用: 人, 小鼠, 大鼠 WB, IF (cc), IHC (p) 宿主: 兔 Monoclonal 11A1 unconjugated
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应用备注
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Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
Immunocytochemistry , 0.5-1 μg/mL, Human, -
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
1. Deak, M., Clifton, A. D., Lucocq, J. M., Alessi, D. R. Mitogen- and stress-activated protein kinase-1 (MSK1) is ly activated by MAPK and SAPK2/p38, and may mediate activation of CREB. EMBO J. 17: 4426-4441, 1998. 2. Jiang, C., Yu, L., Tu, Q., Zhao, Y., Zhang, H., Zhao, S. Assignment of a member of the ribosomal protein S6 kinase family, RPS6KA5, to human chromosome 14q31-q32.1 by radiation hybrid mapping. Cytogenet. Cell Genet. 87: 261-261, 1999. -
说明
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and ICC.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- MSK1 (RPS6KA5) (Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 5 (RPS6KA5))
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别名
- RPS6KA5
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背景
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Synonyms: Ribosomal protein S6 kinase alpha-5,S6K-alpha-5,2.7.11.1,90 kDa ribosomal protein S6 kinase 5,Nuclear mitogen- and stress-activated protein kinase 1,RSK-like protein kinase,RSKL,RPS6KA5,MSK1,
Tissue Specificity: Widely expressed with high levels in heart, brain and placenta. Less abundant in lung, kidney and liver. .
Background: Ribosomal protein S6 kinase alpha-5, also known as MSK1, is an enzyme that in humans is encoded by the RPS6KA5 gene. It belongs to the AGC family of kinases. MSK1 gene is mapped to chromosome 14q31-q32. Northern blot analysis indicated that MSK1 was expressed as a 4-kb mRNA in all tissues tested, with the highest levels of expression in brain, muscle, and placenta. MSK1 was activated in vitro and in vivo by either ERK or SAPK2 proteins. It has been found that MSK1, rather than MAPKAP-K1 or MAPKAP-K2/K3, mediates activation of the cAMP response element-binding protein and activating transcription factor-1 by either growth factors or stress signals.
Sequence Similarities: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.
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分子量
- 90 kDa
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基因ID
- 9252
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UniProt
- O75582
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途径
- MAPK Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades
抗原
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