PD-L1 抗体 (AA 45-290)
Quick Overview for PD-L1 抗体 (AA 45-290) (ABIN3043392)
抗原
See all PD-L1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 45-290
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原理
- Anti-PD-L1/CD274 Antibody Picoband®
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交叉反应 （详细）
- No cross-reactivity with other proteins
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产品特性
- Anti-PD-L1/ Antibody Picoband® (ABIN3043392). Tested in Flow Cytometry, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human PD-L1 recombinant protein (Position: E45-T290). Human PD-L1 shares 69% amino acid (aa) sequence identity with mouse PD-L1.
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亚型
- IgG
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anti-CD274 (PD-L1) (C-Term) antibody
Verified PD-L1 适用: 人 WB, FACS, ELISA, IF 宿主: 山羊 Polyclonal unconjugated
anti-CD274 (PD-L1) (AA 19-238) antibodyPD-L1 适用: 人 WB, FACS, IHC, ELISA, IF 宿主: 小鼠 Monoclonal 9B5F4 unconjugated
anti-CD274 (PD-L1) (AA 39-191) antibodyPD-L1 适用: 人, 小鼠 WB, FACS, IHC, ELISA, IF, Coat, StM 宿主: 小鼠 Monoclonal PDL1-2746 unconjugated
anti-CD274 (PD-L1) (AA 24-153) antibodyPD-L1 适用: 人 FACS, IHC, ELISA 宿主: 小鼠 Monoclonal 6D9A2 unconjugated
anti-CD274 (PD-L1) (AA 48-290) antibodyPD-L1 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-CD274 (PD-L1) (AA 34-241) antibodyPD-L1 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-CD274 (PD-L1) (AA 19-238) antibodyPD-L1 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-CD274 (PD-L1) (C-Term) antibodyKO Validated PD-L1 适用: 人 WB, FACS, IF, ICC, IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-CD274 (PD-L1) (AA 24-153) antibodyPD-L1 适用: 人 FACS, ELISA 宿主: 小鼠 Monoclonal 7D2A10 unconjugated
anti-CD274 (PD-L1) (AA 201-290) antibodyPD-L1 适用: 小鼠, 大鼠 WB, ELISA, IF (cc), IHC (p), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.25-0.5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
1. Barber, D. L., Wherry, E. J., Masopust, D., Zhu, B., Allison, J. P., Sharpe, A. H., Freeman, G. J., Ahmed, R. Restoring function in exhausted CD8 T cells during chronic viral infection. Nature 439: 682-687, 2006. 2. Dong, H., Zhu, G., Tamada, K., Chen, L. B7-H1, a third member of the B7 family, co-stimulates T-cell proliferation and interleukin-10 secretion.Nature Med. 5: 1365-1369, 1999. 3. Curiel, T. J., Wei, S., Dong, H., Alvarez, X., Cheng, P., Mottram, P., Krzysiek, R., Knutson, K. L., Daniel, B., Zimmermann, M. C., David, O., Burow, M., and 10 others. Blockade of B7-H1 improves myeloid dendritic cell-mediated antitumor immunity. Nature Med. 9: 562-567, 2003. -
说明
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- PD-L1 (CD274 (PD-L1))
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别名
- CD274
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背景
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Synonyms: Programmed cell death 1 ligand 1,PD-L1,PDCD1 ligand 1,Programmed death ligand 1,B7 homolog 1,B7-H1,CD274,CD274,B7H1, PDCD1L1, PDCD1LG1, PDL1,
Tissue Specificity: Highly expressed in the heart, skeletal muscle, placenta and lung. Weakly expressed in the thymus, spleen, kidney and liver. Expressed on activated T- and B-cells, dendritic cells, keratinocytes and monocytes. .
Background: Programmed death-ligand 1 (PD-L1) also known as CD274 or B7-H1 is a protein that in humans is encoded by the CD274 gene. It is mapped to 9p24.1. PD-L1 is a 40 kDa type 1 transmembrane protein that has been speculated to play a major role in suppressing the immune system during particular events such as pregnancy, tissue allografts, autoimmune disease and other disease states such as hepatitis. It has been concluded that upregulation of PD-L1 on tumor MDCs downregulates T-cell immunity and that PD-L1 blockade may represent an approach for cancer immunotherapy. PD-L1 can provide positive costimulatory signals for innate and adaptive immunity and for protection against intracellular bacterial infection. What's more, It has been found that PD1/PDL1 pathway may be a good target for restoring antitumor immunity in ovarian cancer.
Sequence Similarities: Belongs to the immunoglobulin superfamily. BTN/MOG family.
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分子量
- 40-50 kDa
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基因ID
- 29126
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途径
- Cancer Immune Checkpoints
抗原
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