电话:
+86 (0512) 65829739
传真:
+86 (010) 6788 5057
电子邮件:
orders@antibodies-online.cn

PAPPA 抗体 (AA 95-388)

This anti-PAPPA antibody is a 兔 多克隆 antibody detecting PAPPA in WB 和 IHC. Suitable for 人.
产品编号 ABIN3043377
发货至: 中国

Quick Overview for PAPPA 抗体 (AA 95-388) (ABIN3043377)

抗原

See all PAPPA 抗体
PAPPA (Pregnancy-Associated Plasma Protein A, Pappalysin 1 (PAPPA))

适用

  • 141
  • 26

宿主

  • 94
  • 56
  • 1

克隆类型

  • 95
  • 56
多克隆

标记

  • 55
  • 20
  • 12
  • 8
  • 6
  • 5
  • 4
  • 4
  • 4
  • 4
  • 4
  • 4
  • 4
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
This PAPPA antibody is un-conjugated

应用范围

  • 75
  • 61
  • 51
  • 42
  • 40
  • 20
  • 13
  • 13
  • 6
  • 3
  • 3
  • 3
  • 3
  • 3
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
Western Blotting (WB), Immunohistochemistry (IHC)
  • 抗原表位

    • 43
    • 15
    • 8
    • 6
    • 4
    • 4
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 95-388

    原理

    Anti-PAPP A/PAPPA Antibody Picoband®

    交叉反应 (详细)

    No cross-reactivity with other proteins

    产品特性

    Anti-PAPP A/PAPPA Antibody Picoband® (ABIN3043377). Tested in IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

    纯化方法

    Immunogen affinity purified.

    免疫原

    E.coli-derived human PAPP A recombinant protein (Position: R95-Q388). Human PAPP A shares 88% amino acid (aa) sequence identity with mouse PAPP A.

    亚型

    IgG
  • 应用备注

    Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human
    Western blot, 0.1-0.5 μg/mL, Human
    1. Oxvig, C., Sand, O., Kristensen, T., Gleich, G. J., Sottrup-Jensen, L. Circulating human pregnancy-associated plasma protein-A is disulfide-bridged to the proform of eosinophil major basic protein. J. Biol. Chem. 268: 12243-12246, 1993. 2. Overgaard, M. T., Haaning, J., Boldt, H. B., Olsen, I. M., Laursen, L. S., Christiansen, M., Gleich, G. J., Sottrup-Jensen, L., Conover, C. A., Oxvig, C. Expression of recombinant human pregnancy-associated plasma protein-A and identification of the proform of eosinophil major basic protein as its physiological inhibitor. J. Biol. Chem. 275: 31128-31133, 2000.

    说明

    Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

    限制

    仅限研究用
  • 状态

    Lyophilized

    溶解方式

    Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

    浓度

    500 μg/mL

    缓冲液

    Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Avoid repeated freezing and thawing.

    储存条件

    4 °C,-20 °C

    储存方法

    Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
    It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
  • 抗原

    PAPPA (Pregnancy-Associated Plasma Protein A, Pappalysin 1 (PAPPA))

    别名

    PAPPA

    背景

    Synonyms: Pappalysin-1,3.4.24.79,Insulin-like growth factor-dependent IGF-binding protein 4 protease,IGF-dependent IGFBP-4 protease,IGFBP-4ase,Pregnancy-associated plasma protein A,PAPP-A,PAPPA,

    Tissue Specificity: High levels in placenta and pregnancy serum. In placenta, expressed in X cells in septa and anchoring villi, and in syncytiotrophoblasts in the chorionic villi. Lower levels are found in a variety of other tissues including kidney, myometrium, endometrium, ovaries, breast, prostate, bone marrow, colon, fibroblasts and osteoblasts. .

    Background: Pappalysin-1, also known as DIPLA1, is a protein that in humans is encoded by the PAPPA gene. It is mapped to 9q33.1. PAPPA is found in the ovarian follicles, follicular fluid, luteal cells, and fallopian tubes of nonpregnant women and in the seminal vesicles and seminal fluid of males. This gene encodes a secreted metalloproteinase which cleaves insulin-like growth factor binding proteins (IGFBPs). It is thought to be involved in local proliferative processes such as wound healing and bone remodeling. Low plasma level of this protein has been suggested as a biochemical marker for pregnancies with aneuploid fetuses. It has been found that circulating PAPPA is a disulfide-bridged complex with proMBP in which the subunits of the constituents are present in a 1:1 Molar ratio.

    Sequence Similarities: Belongs to the peptidase M43B family.

    分子量

    181 kDa

    基因ID

    5069

    UniProt

    Q13219
You are here:
Chat with us!