PKC delta 抗体 (AA 1-160)
(1 reference)Quick Overview for PKC delta 抗体 (AA 1-160) (ABIN3043360)
抗原
See all PKC delta (PKCd) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 1-160
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原理
- Anti-PKC delta/PRKCD Antibody Picoband®
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交叉反应 （详细）
- No cross-reactivity with other proteins
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产品特性
- Anti-PKC delta/PRKCD Antibody (ABIN3043360). Tested in IF, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human PKC delta recombinant protein (Position: M1-E160). Human PKC delta shares 95% and 93% amino acid (aa) sequence identity with mouse and rat PKC delta, respctively.
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亚型
- IgG
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anti-Protein Kinase C, delta (PKCd) (AA 477-676) antibody
PKCd 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Protein Kinase C, delta (PKCd) (AA 577-676) antibodyPKCd 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 2E12 unconjugated
anti-Protein Kinase C, delta (PKCd) (AA 577-676) antibodyPKCd 适用: 人 WB, ELISA, RNAi 宿主: 小鼠 Monoclonal 6A2 unconjugated
anti-Protein Kinase C, delta (PKCd) (C-Term) antibodyPKCd 适用: 人, 小鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Protein Kinase C, delta (PKCd) (pTyr313) antibodyPKCd 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Protein Kinase C, delta (PKCd) antibodyPKCd 适用: 人 WB, IHC, IF 宿主: 兔 Monoclonal unconjugated
anti-Protein Kinase C, delta (PKCd) (pSer645) antibodyPKCd 适用: 人, 小鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Protein Kinase C, delta (PKCd) (pTyr64) antibodyPKCd 适用: 人, 小鼠, 大鼠 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Protein Kinase C, delta (PKCd) (pSer645) antibodyPKCd 适用: 人, 猴 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Protein Kinase C, delta (PKCd) (AA 1-676) antibodyPKCd 适用: 人 WB, PLA 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.1-0.5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
1. Qu, Y., Misaghi, S., Izrael-Tomasevic, A., Newton, K., Gilmour, L. L., Lamkanfi, M., Louie, S., Kayagaki, N., Liu, J., Komuves, L., Cupp, J. E., Arnott, D., Monack, D., Dixit, V. M.Phosphorylation of NLRC4 is critical for inflammasome activation. Nature 490: 539-542, 2012. 2. Salzer, E., Santos-Valente, E., Klaver, S., Ban, S. A., Emminger, W., Prengemann, N. K., Garncarz, W., Mullauer, L., Kain, R., Boztug, H., Heitger, A., Arbeiter, K., Eitelberger, F., Seidel, M. G., Holter, W., Pollak, A., Pickl, W. F., Forster-Waldl, E., Boztug, K. B-cell deficiency and severe autoimmunity caused by deficiency of protein kinase C-delta. Blood 121: 3112-3116, 2013. 3. Tu, X., Joeng, K. S., Nakayama, K. I., Nakayama, K., Rajagopal, J., Carroll, T. J., McMahon, A. P., Long, F. Noncanonical Wnt signaling through G protein-linked PKC-delta activation promotes bone formation. Dev. Cell 12: 113-127, 2007. -
说明
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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: "5-(Bis(3-(2-hydroxyethyl)-1H-indol-2-yl)methyl)-2-hydroxybenzoic acid (BHIMHA): showing a strategy of designing drug to block lung metastasis of tumors." in: Drug design, development and therapy, Vol. 10, pp. 711-21, (2016) (PubMed).
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: "5-(Bis(3-(2-hydroxyethyl)-1H-indol-2-yl)methyl)-2-hydroxybenzoic acid (BHIMHA): showing a strategy of designing drug to block lung metastasis of tumors." in: Drug design, development and therapy, Vol. 10, pp. 711-21, (2016) (PubMed).
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- PKC delta (PKCd) (Protein Kinase C, delta (PKCd))
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别名
- PRKCD
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背景
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Synonyms: Protein kinase C delta type,2.7.11.13,Tyrosine-protein kinase PRKCD,2.7.10.2,nPKC-delta,Protein kinase C delta type regulatory subunit,Protein kinase C delta type catalytic subunit,Sphingosine-dependent protein kinase-1,SDK1,PRKCD,
Tissue Specificity: Localized at tight junctions of both epithelial and endothelial cells. Highly expressed in kidney. Not detected in testis.
Background: Protein kinase C delta type, also called PKCD, is an enzyme that in humans is encoded by the PRKCD gene. The PRKCD gene encodes a member of the protein kinase C family, members of which are critical for regulation of cell survival, proliferation, and apoptosis. This gene is mapped to 3p21.1. Studies both in human and mice demonstrate that this kinase is involved inB cell signaling and in the regulation of growth, apoptosis, and differentiation of a variety of cell types. PRKCD is also regulated by phosphorylation on various tyrosine residues including Y311 (by SRC). It has been showed that PRKCD phosphorylates NLRC4 and that this phosphorylation is critical for inflammasome assembly. What's more, it is also a part of a noncanonical WNT signaling cascade.
Sequence Similarities: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
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分子量
- 77 kDa
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基因ID
- 5580
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UniProt
- Q05655
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途径
- Interferon-gamma Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Thyroid Hormone Synthesis, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Myometrial Relaxation and Contraction, M Phase, G-protein mediated Events, Dicarboxylic Acid Transport, Positive Regulation of Response to DNA Damage Stimulus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, Lipid Metabolism
抗原
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