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Osteopontin 抗体 (C-Term)

Name: Osteopontin 抗体 (C-Term)
SKU: ABIN3043353
Availability: OutOfStock

Cited in 4+ publications. The 兔多克隆 anti-Osteopontin antibody (ABIN3043353) specifically detects Osteopontin in WB 和 ELISA. The antibody is reactive with 人, 小鼠和大鼠 samples.

Western Blotting (WB) image for anti-Secreted phosphoprotein 1 (SPP1) (AA 281-314), (C-Term) antibody (ABIN3043353) (Osteopontin 抗体 (C-Term))

Western blot analysis of OPN using anti- OPN antibody . (Osteopontin 抗体 (C-Term))

2 images

(4 references)

产品编号 ABIN3043353

发货至: 中国

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Quick Overview for Osteopontin 抗体 (C-Term) (ABIN3043353)

抗原

See all Osteopontin (SPP1) 抗体

Osteopontin (SPP1) (Secreted phosphoprotein 1 (SPP1))

适用

人, 小鼠, 大鼠

宿主

兔

克隆类型

多克隆

标记

This Osteopontin antibody is un-conjugated

应用范围

Western Blotting (WB), ELISA

Discover our top product SPP1 Primary Antibody

抗原表位
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AA 281-314, C-Term

原理

Anti-Osteopontin/SPP1 Antibody

序列

HEDMLVVDPK SKEEDKHLKF RISHELDSAS SEVN

交叉反应（详细）

No cross-reactivity with other proteins

产品特性

Anti-Osteopontin/SPP1 Antibody (ABIN3043353). Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

纯化方法

Immunogen affinity purified.

免疫原

A synthetic peptide corresponding to a sequence at the C-terminus of human Osteopontin, different from the related mouse sequence by eight amino acids, and from the related rat sequence by seven amino acids.

亚型

IgG
anti-Secreted phosphoprotein 1 (SPP1) (AA 17-317) antibody
SPP1 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Secreted phosphoprotein 1 (SPP1) (AA 18-314) antibody
SPP1 适用: 猕猴 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Secreted phosphoprotein 1 (SPP1) (AA 1-300) antibody
SPP1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 4F2 unconjugated

anti-Secreted phosphoprotein 1 (SPP1) (AA 167-314) antibody
SPP1 适用: 人 IHC, ELISA, ICC 宿主: 小鼠 Monoclonal 7C5H12 unconjugated

anti-Secreted phosphoprotein 1 (SPP1) antibody
SPP1 适用: 人 WB, FACS, IF (p) 宿主: 兔 Monoclonal 24GB3955 unconjugated Recombinant Antibody

anti-Secreted phosphoprotein 1 (SPP1) antibody
SPP1 适用: 人 WB, IHC, ELISA, IF 宿主: 小鼠 Monoclonal 7C5H12 unconjugated

anti-Secreted phosphoprotein 1 (SPP1) (AA 167-314) antibody
SPP1 适用: 人 WB, IHC, ELISA, IF/ICC 宿主: 小鼠 Monoclonal unconjugated

anti-Secreted phosphoprotein 1 (SPP1) (AA 221-280) antibody
SPP1 适用: 小鼠, 大鼠 WB, ELISA, IHC (p), IF (p), IF (cc), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Secreted phosphoprotein 1 (SPP1) (C-Term) antibody
Verified SPP1 适用: 人 WB, ELISA 宿主: 山羊 Polyclonal unconjugated

anti-Secreted phosphoprotein 1 (SPP1) (AA 1-300) antibody
SPP1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1F5 unconjugated

应用备注

Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat, -
ELISA, 0.1-0.5 μg/mL, -

说明

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

限制

仅限研究用
状态

Lyophilized

溶解方式

Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

浓度

500 μg/mL

缓冲液

Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Avoid repeated freezing and thawing.

储存条件

4 °C,-20 °C

储存方法

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Hou, Huang, Luo, Wang, Liu, Deng, Zhang, Liu, Chen: "MiR-351 negatively regulates osteoblast differentiation of MSCs induced by (+)-cholesten-3-one through targeting VDR." in: American journal of translational research, Vol. 9, Issue 11, pp. 4963-4973, (2017) (PubMed).

Qu, Hui, Wang, Tang, Zhong, Liu, Li, Feng, He et al.: "Reduced Expression of the Extracellular Calcium-Sensing Receptor (CaSR) Is Associated with Activation of the Renin-Angiotensin System (RAS) to Promote Vascular Remodeling in the Pathogenesis of ..." in: PLoS ONE, Vol. 11, Issue 7, pp. e0157456, (2016) (PubMed).

Yin, Cheng, Qin, Yu, Yu, Zhong, Sun, Zhang: "Effects of Naringin on Proliferation and Osteogenic Differentiation of Human Periodontal Ligament Stem Cells In Vitro and In Vivo." in: Stem cells international, Vol. 2015, pp. 758706, (2015) (PubMed).

Shan, Zhou, He, Feng, Chen, Zhong: "Expression of both matrix metalloproteinase-2 and its tissue inhibitor-2 in tunica media of radial artery in uremic patients." in: Renal failure, Vol. 35, Issue 1, pp. 37-42, (2013) (PubMed).
抗原

Osteopontin (SPP1) (Secreted phosphoprotein 1 (SPP1))

别名

SPP1

背景

Synonyms: Osteopontin,Bone sialoprotein 1,Nephropontin,Secreted phosphoprotein 1,SPP-1,Urinary stone protein,Uropontin,SPP1,BNSP, OPN,PSEC0156,
Tissue Specificity: Bone. Found in plasma.
Background: Osteopontin (OPN), also known as secreted phosphoprotein 1 (SPP1), is a protein that in humans is encoded by the SPP1 gene (secreted phosphoprotein 1). The protein encoded by this gene is involved in the attachment of osteoclasts to the mineralized bone matrix. And the encoded protein is secreted and binds hydroxyapatite with high affinity. The osteoclast vitronectin receptor is found in the cell membrane and may be involved in the binding to this protein. Also, this protein is a cytokine that upregulates expression of interferon-gamma and interleukin-12. Several transcript variants encoding different isoforms have been found for this gene.
Sequence Similarities: Belongs to the osteopontin family.

分子量

66 kDa, 25-55 kDa

基因ID

6696

UniProt

P10451

途径

Regulation of Cell Size