Dystrophin 抗体 (AA 3076-3404)
Quick Overview for Dystrophin 抗体 (AA 3076-3404) (ABIN3043317)
抗原
See all Dystrophin (DMD) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 3076-3404
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原理
- Anti-Dystrophin/DMD Antibody Picoband®
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交叉反应 （详细）
- No cross-reactivity with other proteins
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产品特性
- Anti-Dystrophin/DMD Antibody Picoband® (ABIN3043317). Tested in Flow Cytometry, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human Dystrophin recombinant protein (Position: H3076-D3404). Human Dystrophin shares 100% amino acid (aa) sequence identity with mouse Dystrophin.
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亚型
- IgG
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anti-Dystrophin (DMD) (AA 346-635) antibody
DMD 适用: 人 IHC, WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Dystrophin (DMD) antibodyDMD 适用: 人, 小鼠, 大鼠 IHC, WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Dystrophin (DMD) (AA 1-635) antibodyDMD 适用: 人 WB, ELISA, IP 宿主: 小鼠 Monoclonal 3A11 unconjugated
anti-Dystrophin (DMD) (AA 114-263) antibodyDMD 适用: 人 IHC, ELISA, Coat, StM 宿主: 小鼠 Monoclonal DMD-3242 unconjugated
anti-Dystrophin (DMD) (AA 3048-3328) antibodyDMD 适用: 人 IHC, WB, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Dystrophin (DMD) (AA 114-263) antibodyDMD 适用: 人 IHC, ELISA, Coat, StM 宿主: 小鼠 Monoclonal DMD-3241 unconjugated
anti-Dystrophin (DMD) (AA 114-263) antibodyDMD 适用: 人 IHC, ELISA, Coat, StM 宿主: 小鼠 Monoclonal DMD-3245 unconjugated
anti-Dystrophin (DMD) (AA 253-597) antibodyDMD 适用: 人 IHC, WB, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Dystrophin (DMD) (AA 3400-3558) antibodyDMD 适用: 人 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Dystrophin (DMD) antibodyDMD 适用: 人 IHC, ELISA 宿主: 兔 Monoclonal 4B10 unconjugated Recombinant Antibody
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应用备注
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Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Frozen Section), 0.5-1 μg/mL, Human
Immunocytochemistry, 0.5-1 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human1. Tennyson CN, Klamut HJ, Worton RG (1995). "The human dystrophin gene requires 16 hours to be transcribed and is cotranscriptionally spliced". Nature Genetics 9 (2): 184-90. 2. García-Pelagio KP, Bloch RJ, Ortega A, González-Serratos H (2011). "Biomechanics of the sarcolemma and costameres in single skeletal muscle fibers from normal and dystrophin- null mice". J Muscle Res Cell Motil 31 (5-6): 323-336. 3. Strachan T and Read AP, 1999. Human molecular genetics, BIOS Scientific, New York, USA -
说明
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- Dystrophin (DMD)
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别名
- DMD
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背景
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Synonyms: Dystrophin,DMD,
Tissue Specificity: Expressed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma. Expressed in brain, muscle, kidney, lung and testis. Isoform 5 is expressed in heart, brain, liver, testis and hepatoma cells. Most tissues contain transcripts of multiple isoforms, however only isoform 5 is detected in heart and liver. .
Background: Dystrophin, also known as DMD, is a rod-shaped cytoplasmic protein, and a vital part of a protein complex that connects the cytoskeleton of a muscle fiber to the surrounding extracellular matrix through the cell membrane. It is mapped to Xp21.2-p21.1. This complex is variously known as the costamere or thedystrophin-associated protein complex. Many muscle proteins, such as α-dystrobrevin, syncoilin, synemin, sarcoglycan, dystroglycan, and sarcospan, colocalize with dystrophin at the costamere. Dystrophin is a protein located between the sarcolemma and the outermost layer of myofilaments in the muscle fiber (myofiber). It is a cohesive protein, linking actin filaments to another support protein that resides on the inside surface of each muscle fiber's plasma membrane (sarcolemma).
Sequence Similarities: Contains 2 CH (calponin-homology) domains.
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分子量
- 427 kDa
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基因ID
- 1756
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UniProt
- P11532
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途径
- Skeletal Muscle Fiber Development
抗原
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