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Leptin 抗体 (Middle Region)

Name: Leptin 抗体 (Middle Region)
SKU: ABIN3043294
Availability: OutOfStock

This anti-Leptin antibody is a 兔多克隆 antibody detecting Leptin in WB 和 ELISA. Suitable for 小鼠. This Primary Antibody has been cited in 4+ publications.

Observed bind size: 16KD (Leptin 抗体 (Middle Region))

1 image

(4 references)

产品编号 ABIN3043294

发货至: 中国

Quick Overview for Leptin 抗体 (Middle Region) (ABIN3043294)

抗原

See all Leptin (LEP) 抗体

Leptin (LEP)

适用

小鼠

宿主

兔

克隆类型

多克隆

标记

This Leptin antibody is un-conjugated

应用范围

Western Blotting (WB), ELISA

Discover our top product LEP Primary Antibody

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AA 74-109, Middle Region

原理

Anti-Leptin Antibody Picoband®

序列

KMDQTLAVYQ QVLTSLPSQN VLQIANDLEN LRDLLH

交叉反应（详细）

No cross-reactivity with other proteins

产品特性

Anti-Leptin Antibody Picoband® (ABIN3043294). Tested in ELISA, WB applications. This antibody reacts with Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

纯化方法

Immunogen affinity purified.

免疫原

A synthetic peptide corresponding to a sequence in the middle region of mouse Leptin, different from the related human sequence by five amino acids, and from the related rat sequence by two amino acids.

亚型

IgG
anti-Leptin (LEP) (AA 22-167) antibody
LEP 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Leptin (LEP) (AA 22-167) antibody
LEP 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Leptin (LEP) (AA 17-100) antibody
LEP 适用: 人, 小鼠, 大鼠 WB, ELISA, IF (p), IF (cc), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Leptin (LEP) (AA 8-37) antibody
LEP 适用: 人 WB, IHC, ELISA, IF, FACS 宿主: 兔 Polyclonal unconjugated

anti-Leptin (LEP) (AA 22-167) antibody
LEP 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Leptin (LEP) (AA 29-160) antibody
LEP 适用: Cow WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Leptin (LEP) (AA 22-167) antibody
LEP 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated

anti-Leptin (LEP) (AA 7-37), (N-Term) antibody
LEP 适用: 人 WB, IF, FACS, EIA, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-Leptin (LEP) (AA 101-154) antibody
LEP 适用: 小鼠, 大鼠 ELISA, IF (p), IF (cc), FACS, IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Leptin (LEP) (AA 22-167) antibody
LEP 适用: 人 WB 宿主: 小鼠 Monoclonal C5 unconjugated

应用备注

ELISA, 0.1-0.5 μg/mL, -
Western blot, 0.1-0.5 μg/mL, Mouse
1. Halaas JL, Gajiwala KS, Maffei M, Cohen SL, Chait BT, Rabinowitz D, Lallone RL, Burley SK, Friedman JM (July 1995). "Weight-reducing effects of the plasma protein encoded by the obese gene". Science 269 (5223): 543-6. 2. Maffei M, Halaas J, Ravussin E, Pratley RE, Lee GH, Zhang Y, Fei H, Kim S, Lallone R, Ranganathan S (November 1995). "Leptin levels in human and rodent: measurement of plasma leptin and ob RNA in obese and weight-reduced subjects". Nat. Med. 1 (11): 1155-61. 3. Pan H, Guo J, Su Z (May 2014). "Advances in understanding the interrelations between leptin resistance and obesity".Physiology & Behavior 130: 157-169.

说明

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

限制

仅限研究用
状态

Lyophilized

溶解方式

Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

浓度

500 μg/mL

缓冲液

Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Avoid repeated freezing and thawing.

储存条件

4 °C,-20 °C

储存方法

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Li, Zhao, Qi, Wang, Zhang, Li, Qin: "lncRNA Ftx promotes aerobic glycolysis and tumor progression through the PPARγ pathway in hepatocellular carcinoma." in: International journal of oncology, Vol. 53, Issue 2, pp. 551-566, (2018) (PubMed).

Qin, Ma, Yang, Hu, Zhou, Fu, Tian, Liu, Xu, Shen: "A Triterpenoid Inhibited Hormone-Induced Adipocyte Differentiation and Alleviated Dexamethasone-Induced Insulin Resistance in 3T3-L1 adipocytes." in: Natural products and bioprospecting, Vol. 5, Issue 3, pp. 159-66, (2015) (PubMed).

Yuan, Zhang, Cai, Ding, Wang, Chen, Wang, Yan, Lu: "Leptin induces cell proliferation and reduces cell apoptosis by activating c-myc in cervical cancer." in: Oncology reports, Vol. 29, Issue 6, pp. 2291-6, (2013) (PubMed).

Cheng, Dai, Dai: "Testis dysfunction by isoproterenol is mediated by upregulating endothelin receptor A, leptin and protein kinase Cvarepsilon and is attenuated by an endothelin receptor antagonist CPU0213." in: Reproductive toxicology (Elmsford, N.Y.), Vol. 29, Issue 4, pp. 421-6, (2010) (PubMed).
抗原

Leptin (LEP)

别名

Lep

背景

Synonyms: Leptin,Obesity factor,Lep,Ob,
Tissue Specificity: Detected in blood plasma (PubMed:8820107). Produced and secreted by astrocytes (at protein level) (PubMed:19065001). Abundantly expressed in liver, brain and testis with highest levels in liver. In the brain, found in cerebellum, cerebral cortex, hippocampus and brain stem. Located to neurons and neuroglia. .
Background: Leptin is a protein product of the mouse obese gene. Mice with mutations in the obese gene that block the synthesis of Leptin have been found to be obese and diabetic and to have reduced activity, metabolism and body temperature. cDNA clones encoding Leptin have been isolated from human, simian, mouse, and rat cells. The expression of Leptin mRNA has been shown to be restricted to adipose tissue. Although regulation of fat stores is deemed to be the primary function of leptin, it also plays a role in other physiological processes, as evidenced by its multiple sites of synthesis other than fat cells, and the multiple cell types beside hypothalamic cells that have leptin receptors. Many of these additional functions are yet to be defined.
Sequence Similarities: Contains 1 HMG box DNA-binding domain.

分子量

16 kDa

基因ID

16846

UniProt

P41160

途径

JAK/STAT Signaling, AMPK Signaling, Hormone Transport, Peptide Hormone Metabolism, Hormone Activity, Negative Regulation of Hormone Secretion, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Monocarboxylic Acid Catabolic Process