Dihydrofolate Reductase 抗体 (AA 2-187)
(1 reference)Quick Overview for Dihydrofolate Reductase 抗体 (AA 2-187) (ABIN3043286)
抗原
See all Dihydrofolate Reductase (DHFR) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 2-187
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原理
- Anti-Dihydrofolate reductase (DHFR) Antibody Picoband®
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交叉反应 （详细）
- No cross-reactivity with other proteins
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产品特性
- Anti-Dihydrofolate reductase (DHFR) Antibody Picoband® (ABIN3043286). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human DHFR recombinant protein (Position: V2-D187). Human DHFR shares 90% amino acid (aa) sequence identity with both mouse and rat DHFR.
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亚型
- IgG
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anti-Dihydrofolate Reductase (DHFR) (AA 88-187) antibody
DHFR 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 2B10 unconjugated
anti-Dihydrofolate Reductase (DHFR) antibodyDHFR 适用: 人, 犬, 猴 WB, IHC, IF, IHC (p) 宿主: 小鼠 Monoclonal 6G7 unconjugated
anti-Dihydrofolate Reductase (DHFR) antibodyDHFR 适用: 人 WB, ELISA, IHC, IP 宿主: 兔 Monoclonal 9B2 unconjugated Recombinant Antibody
anti-Dihydrofolate Reductase (DHFR) (AA 135-164) antibodyDHFR 适用: 小鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Dihydrofolate Reductase (DHFR) (AA 1-50) antibodyDHFR 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Dihydrofolate Reductase (DHFR) (AA 16-30), (N-Term) antibodyDHFR 适用: 人, 小鼠, 大鼠 WB, IHC (p) 宿主: 兔 Polyclonal RB49346 unconjugated
anti-Dihydrofolate Reductase (DHFR) antibodyDHFR 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Dihydrofolate Reductase (DHFR) (AA 1-187) antibodyDHFR 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Dihydrofolate Reductase (DHFR) antibodyDHFR 适用: 人 WB, IHC (p), IF (p), FACS 宿主: 兔 Polyclonal unconjugated
anti-Dihydrofolate Reductase (DHFR) (AA 2-187) antibodyDHFR 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
1. Chen MJ, Shimada T, Moulton AD, Harrison M, Nienhuis AW (December 1982). "Intronless human dihydrofolate reductase genes are derived from processed RNA molecules". Proc. Natl. Acad. Sci. U.S.A. 79 (23): 7435-9. 2. Chen MJ, Shimada T, Moulton AD, Cline A, Humphries RK, Maizel J, Nienhuis AW (March 1984). "The functional human dihydrofolate reductase gene". J. Biol. Chem. 259 (6): 3933-43. 3. Funanage VL, Myoda TT, Moses PA, Cowell HR (October 1984). "Assignment of the human dihydrofolate reductase gene to the q11----q22 region of chromosome 5". Mol. Cell. Biol. 4 (10): 2010-6. -
说明
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.01 mg Sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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: "MicroRNA-205 suppresses proliferation and promotes apoptosis in laryngeal squamous cell carcinoma." in: Medical oncology (Northwood, London, England), Vol. 31, Issue 1, pp. 785, (2013) (PubMed).
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: "MicroRNA-205 suppresses proliferation and promotes apoptosis in laryngeal squamous cell carcinoma." in: Medical oncology (Northwood, London, England), Vol. 31, Issue 1, pp. 785, (2013) (PubMed).
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- Dihydrofolate Reductase (DHFR)
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别名
- DHFR
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背景
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Synonyms: Dihydrofolate reductase,1.5.1.3,DHFR,
Tissue Specificity: Widely expressed in fetal and adult tissues, including throughout the fetal and adult brains and whole blood. Expression is higher in the adult brain than in the fetal brain. .
Background: Dihydrofolate reductase, or DHFR, is an enzyme that reduces dihydrofolic acid to tetrahydrofolic acid, using NADPH as electron donor, which can be converted to the kinds of tetrahydrofolate cofactors used in 1-carbon transfer chemistry. In humans, the DHFR enzyme is encoded by the DHFR gene. It is found in the q11→q22 region of chromosome 5. What's more, DHFR belongs to the dihydrofolate reductase family, and it converts dihydrofolate into tetrahydrofolate, a methyl group shuttle required for the de novo synthesis of purines, thymidylic acid, and certain amino acids. DHFR is the key enzyme in folate metabolism. In addition, DHFR catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis.
Sequence Similarities: Belongs to the dihydrofolate reductase family.
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分子量
- 21 kDa
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基因ID
- 1719
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UniProt
- P00374
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途径
- Mitotic G1-G1/S Phases
抗原
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