T-Bet 抗体 (C-Term)
(1 reference)Quick Overview for T-Bet 抗体 (C-Term) (ABIN3042957)
抗原
See all T-Bet 抗体适用
宿主
克隆类型
标记
应用范围
-
-
抗原表位
- AA 522-535, C-Term
-
原理
- Anti-T-bet/Tbx21 Antibody Picoband®
-
序列
- DKEAEGQFYN YFPN
-
交叉反应 （详细）
- No cross-reactivity with other proteins
-
产品特性
- Anti-T-bet/Tbx21 Antibody (ABIN3042957). Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
-
纯化方法
- Immunogen affinity purified.
-
免疫原
- A synthetic peptide corresponding to a sequence at the C-terminus of human T-bet, different from the related rat and mouse sequences by one amino acid.
-
亚型
- IgG
-
-
anti-T-Bet (AA 241-493) antibody
适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-T-Bet (Internal Region) antibody适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-T-Bet (AA 338-387) antibody适用: 人, 大鼠, Cow, 犬, Pig, 兔, 豚鼠, 猴, Bat WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-T-Bet (Middle Region) antibody适用: 人, 小鼠, 大鼠, Cow, 犬, Pig, 兔, 豚鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-T-Bet antibody适用: 人, 小鼠, 大鼠 WB, IF (p), IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-T-Bet (AA 160-399) antibody适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-T-Bet (AA 387-486) antibody适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 4F8 unconjugated
anti-T-Bet (AA 400-535) antibody适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 2F10 unconjugated
anti-T-Bet (AA 326-535) antibody适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-T-Bet (AA 82-395) antibody适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
-
-
应用备注
-
Western blot, 0.1-0.5 μg/mL, Human, Rat, Mouse
1. Finotto, S., Neurath, M. F., Glickman, J. N., Qin, S., Lehr, H. A., Green, F. H. Y., Ackerman, K., Haley, K., Galle, P. R., Szabo, S. J., Drazen, J. M., De Sanctis, G. T., Glimcher, L. H. Development of spontaneous airway changes consistent with human asthma in mice lacking T-bet. Science 295: 336-338, 2002. 2. Szabo, S. J., Kim, S. T., Costa, G. L., Zhang, X., Fathman, C. G., Glimcher, L. H. A novel transcription factor, T-bet, s Th1 lineage commitment. Cell 100: 655-669, 2000. 3. Zhang, W., Yang, S. Y. Cloning and characterization of a new member of the T-box gene family. Genomics 70: 41-48, 2000. -
说明
-
Antibody can be supported by chemiluminescence kit ABIN921124 in WB.
-
限制
- 仅限研究用
-
-
-
状态
- Lyophilized
-
溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
-
浓度
- 500 μg/mL
-
缓冲液
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.
-
储存液
- Thimerosal (Merthiolate), Sodium azide
-
注意事项
- This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
-
注意事项
- Avoid repeated freezing and thawing.
-
储存条件
- 4 °C,-20 °C
-
储存方法
-
Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles. -
有效期
- 12 months
-
-
-
: "T-Bet Mediated Anti-Neoplastic Effects of Dendritic Cell-Cytokine Induced Killer Cells in vitro." in: Iranian journal of pediatrics, Vol. 22, Issue 1, pp. 43-51, (2012) (PubMed).
-
: "T-Bet Mediated Anti-Neoplastic Effects of Dendritic Cell-Cytokine Induced Killer Cells in vitro." in: Iranian journal of pediatrics, Vol. 22, Issue 1, pp. 43-51, (2012) (PubMed).
-
- T-Bet
-
别名
- TBX21
-
背景
-
Synonyms: T-box transcription factor TBX21,T-box protein 21,T-cell-specific T-box transcription factor T-bet,Transcription factor TBLYM,TBX21,TBET, TBLYM,
Tissue Specificity: T-cell specific.
Background: TBX21 (T-Box 21), also called TBET, is a protein that in humans is encoded by the TBX21 gene. This gene is a member of a phylogenetically conserved family of genes that share a common DNA-binding domain, the T-box. Zhang and Yang (2000) mapped the mouse Tbx21 gene to chromosome 11D in an area showing homology of synteny with human chromosome 17. Szabo et al. (2000) showed that TBX21 expression correlates with IFNG expression in Th1 and natural killer (NK) cells. Ectopic expression of TBX21 both transactivated the IFNG gene and induced endogenous IFNG production. Finotto et al. (2002) observed reduced expression of the T (H)1 transcription factor Tbet in T cells from airways of patients with asthma compared with that in T cells from airways of nonasthmatic patients, suggesting that loss of Tbet might be associated with asthma.
Sequence Similarities: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family.
-
分子量
- 58-60 kDa
抗原
-
