TOLLIP 抗体 (C-Term)
(1 reference)Quick Overview for TOLLIP 抗体 (C-Term) (ABIN3042954)
抗原
See all TOLLIP 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 224-243, C-Term
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原理
- Anti-Toll-interacting protein Tollip Antibody Picoband®
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序列
- NAQPRCSEED LKAIQDMFPN
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交叉反应 （详细）
- No cross-reactivity with other proteins
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产品特性
- Anti-Toll-interacting protein Tollip Antibody (ABIN3042954). Tested in Flow Cytometry, ICC, IHC, IF, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- A synthetic peptide corresponding to a sequence at the C-terminus of human Tollip.
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亚型
- IgG
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anti-Toll Interacting Protein (TOLLIP) (AA 1-274) antibody
TOLLIP 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Toll Interacting Protein (TOLLIP) (AA 1-274) antibodyTOLLIP 适用: 人 WB, ELISA, IP 宿主: 小鼠 Monoclonal 1A5-2A3 unconjugated
anti-Toll Interacting Protein (TOLLIP) (AA 19-254) antibodyTOLLIP 适用: 小鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Toll Interacting Protein (TOLLIP) (AA 1-274) antibodyTOLLIP 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-Toll Interacting Protein (TOLLIP) (AA 1-274) antibodyTOLLIP 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Toll Interacting Protein (TOLLIP) (AA 221-250), (C-Term) antibodyTOLLIP 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal RB4409 unconjugated
anti-Toll Interacting Protein (TOLLIP) (AA 28-261) antibodyTOLLIP 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Toll Interacting Protein (TOLLIP) (AA 86-163) antibodyTOLLIP 适用: 人 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Toll Interacting Protein (TOLLIP) (AA 61-274) antibodyTOLLIP 适用: 人 WB, ELISA, IF, ICC 宿主: 小鼠 Monoclonal k1B17 unconjugated
anti-Toll Interacting Protein (TOLLIP) (AA 61-274) antibodyTOLLIP 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal k1B17 unconjugated
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应用备注
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Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
1. Bulut, Y., Faure, E., Thomas, L., Equils, O., Arditi, M. Cooperation of Toll-like receptor 2 and 6 for cellular activation by soluble tuberculosis factor and Borrelia burgdorferi outer surface protein A lipoprotein: role of Toll-interacting protein and IL-1 receptor signaling molecules in Toll-like receptor 2 signaling. J. Immun. 167: 987-994, 2001. 2. Burns, K., Clatworthy, J., Martin, L., Martinon, F., Plumpton, C., Maschera, B., Lewis, A., Ray, K., Tschopp, J., Volpe, F. Tollip, a new component of the IL-1RI pathway, links IRAK to the IL-1 receptor. Nature Cell Biol. 2: 346-351, 2000. 3. Lo, Y.-L. S., Beckhouse, A. G., Boulus, S. L., Wells, C. A. Diversification of TOLLIP isoforms in mouse and man. Mammalian Genome 20: 305-314, 2009. -
说明
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles. -
有效期
- 12 months
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: "Xuebijing exerts protective effects on lung permeability leakage and lung injury by upregulating Toll-interacting protein expression in rats with sepsis." in: International journal of molecular medicine, Vol. 34, Issue 6, pp. 1492-504, (2014) (PubMed).
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: "Xuebijing exerts protective effects on lung permeability leakage and lung injury by upregulating Toll-interacting protein expression in rats with sepsis." in: International journal of molecular medicine, Vol. 34, Issue 6, pp. 1492-504, (2014) (PubMed).
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- TOLLIP (Toll Interacting Protein (TOLLIP))
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别名
- TOLLIP
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背景
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Synonyms: Toll-interacting protein,TOLLIP,
Tissue Specificity: Abundantly expressed in thymocytes and lymphocytes. Detected in lymphocyte-rich tissues such as thymus, colon, intestine, and spleen. Also found in the prostate.
Background: TOLLIP (TOLL-Interacting Protein), is an inhibitory adaptor protein that in humans is encoded by the TOLLIP gene. Lo et al. (2009) stated that the TOLLIP gene maps to chromosome 11. The mouse gene maps to chromosome 7. By Western blot analysis of embryonic kidney cells, Burns et al. (2000) confirmed the binding of TOLLIP to IL1RAP, to a complex of IL1RAP-IL1R1, and to IL18R. Burns et al. (2000) proposed that IL1B stimulation induces aggregation of IL1Rs, recruitment of MYD88 followed by TOLLIP-IRAK complexes, and the phosphorylation of IRAK by MYD88. This leads to the dissociation of TOLLIP from IRAK, which can then transmit the IL1-induced signals.
Sequence Similarities: Belongs to the tollip family.
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分子量
- 30 kDa
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UniProt
- Q9H0E2
抗原
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