RRM2B 抗体 (C-Term)
Quick Overview for RRM2B 抗体 (C-Term) (ABIN3042928)
抗原
See all RRM2B 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 318-332, C-Term
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原理
- Anti-p53R2/RRM2B Antibody Picoband®
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序列
- EGKTNFFEKR VSEYQ
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交叉反应 （详细）
- No cross-reactivity with other proteins
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产品特性
- Anti-p53R2/RRM2B Antibody (ABIN3042928). Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- A synthetic peptide corresponding to a sequence at the C-terminus of human p53R2, identical to the related rat and mouse sequences.
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亚型
- IgG
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anti-Ribonucleotide Reductase M2 B (TP53 Inducible) (RRM2B) (AA 3-84) antibody
RRM2B 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 6C1 unconjugated
anti-Ribonucleotide Reductase M2 B (TP53 Inducible) (RRM2B) (AA 1-351) antibodyRRM2B 适用: 人 WB, ELISA, IHC, IP 宿主: 兔 Polyclonal unconjugated
anti-Ribonucleotide Reductase M2 B (TP53 Inducible) (RRM2B) (N-Term) antibodyRRM2B 适用: 人, 大鼠, 小鼠 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Ribonucleotide Reductase M2 B (TP53 Inducible) (RRM2B) (AA 3-84) antibodyRRM2B 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 6C1 unconjugated
anti-Ribonucleotide Reductase M2 B (TP53 Inducible) (RRM2B) (N-Term) antibodyRRM2B 适用: 人 WB, ELISA, IHC, IP, FM 宿主: 兔 Polyclonal unconjugated
anti-Ribonucleotide Reductase M2 B (TP53 Inducible) (RRM2B) (C-Term) antibodyRRM2B 适用: 人, 大鼠, 小鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Ribonucleotide Reductase M2 B (TP53 Inducible) (RRM2B) (AA 2-17), (N-Term) antibodyRRM2B 适用: 人, 大鼠, 小鼠 WB, IHC (p), EIA 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.1-0.5 μg/mL, Human, Rat, Mouse
1. Kimura, T., Takeda, S., Sagiya, Y., Gotoh, M., Nakamura, Y., Arakawa, H. Impaired function of p53R2 in Rrm2b-null mice causes severe renal failure through attenuation of dNTP pools. Nature Genet. 34: 440-445, 2003. 2. Kollberg, G., Darin, N., Benan, K., Moslemi, A.-R., Lindal, S., Tulinius, M., Oldfors, A., Holme, E. A novel homozygous RRM2B missense mutation in association with severe mtDNA depletion. Neuromusc. Disord. 19: 147-150, 2009. 3. Takata, A., Kato, M., Nakamura, M., Yoshikawa, T., Kanba, S., Sano, A., Kato, T. Exome sequencing identifies a novel missense variant in RRM2B associated with autosomal recessive progressive external ophthalmoplegia. Genome Biol. 12: R92, 2011. -
说明
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.
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储存液
- Thimerosal (Merthiolate), Sodium azide
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注意事项
- This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles. -
有效期
- 12 months
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- RRM2B (Ribonucleotide Reductase M2 B (TP53 Inducible) (RRM2B))
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别名
- RRM2B
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背景
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Synonyms: Ribonucleoside-diphosphate reductase subunit M2 B,1.17.4.1,TP53-inducible ribonucleotide reductase M2 B,p53-inducible ribonucleotide reductase small subunit 2-like protein,p53R2,RRM2B,P53R2,
Tissue Specificity: Widely expressed at a high level in skeletal muscle and at a weak level in thymus. Expressed in epithelial dysplasias and squamous cell carcinoma. .
Background: RRM2B (Ribonucleotide Reductase, M2 B), also known as P53R2, is an enzyme that in humans is encoded by the RRM2B gene. Tanaka et al. (2000) mapped the p53R2 gene to chromosome 8q23.1 by fluorescence in situ hybridization. Tanaka et al. (2000) found that expression of p53R2, but not R2, was induced by ultraviolet and gamma-irradiation and adriamycin treatment in a wildtype p53-dependent manner. Induction of p53R2 in p53-deficient cells caused G2/M arrest and protected cells from death in response to adriamycin. Inhibition of endogenous p53R2 expression in cells that had an intact p53-dependent DNA damage checkpoint reduced ribonucleotide reductase activity, DNA repair, and cell survival after exposure to various genotoxins.
Sequence Similarities: Belongs to the ribonucleoside diphosphate reductase small chain family.
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分子量
- 83 kDa
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途径
- p53 Pathway, Negative Regulation of intrinsic apoptotic Signaling
抗原
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