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CDK4 抗体 (C-Term)

Name: CDK4 抗体 (C-Term)
SKU: ABIN3042691
Availability: OutOfStock

This anti-CDK4 antibody is a 兔多克隆 antibody detecting CDK4 in WB, IF, FACS 和 ICC. Suitable for 人, 小鼠和大鼠. This Primary Antibody has been cited in 10+ publications.

Anti-Cdk4 antibody, Western blotting Lane 1: Rat Thymus Tissue Lysate Lane 2: HELA Cell Lysate Lane 3: MCF-7 Cell Lysate Lane 4: A549 Cell Lysate Lane 5: COLO320 Cell Lysate Lane 6: JURKAT Cell Lysate (CDK4 抗体 (C-Term))

1 image

(10 references)

产品编号 ABIN3042691

发货至: 中国

Datasheet as PDF

Quick Overview for CDK4 抗体 (C-Term) (ABIN3042691)

抗原

See all CDK4 抗体

CDK4 (Cyclin-Dependent Kinase 4 (CDK4))

适用

人, 小鼠, 大鼠

宿主

兔

克隆类型

多克隆

标记

This CDK4 antibody is un-conjugated

应用范围

Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Discover our top product CDK4 Primary Antibody

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AA 283-303, C-Term

原理

Anti-Cyclin-dependent kinase 4 Cdk4 Antibody Picoband®

序列

RISAFRALQH SYLHKEESDA E

交叉反应（详细）

No cross-reactivity with other proteins

产品特性

Anti-Cyclin-dependent kinase 4 Cdk4 Antibody (ABIN3042691). Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

纯化方法

Immunogen affinity purified.

免疫原

A synthetic peptide corresponding to a sequence at the C-terminus of mouse Cdk4, different from the related rat sequence by one amino acid, and from the related human sequence by four amino acids.

亚型

IgG
anti-Cyclin-Dependent Kinase 4 (CDK4) antibody
KO Validated CDK4 适用: 人 WB, IF, ICC, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-Cyclin-Dependent Kinase 4 (CDK4) (N-Term) antibody
CDK4 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-Cyclin-Dependent Kinase 4 (CDK4) (AA 211-303) antibody
CDK4 适用: 人 WB, ELISA, IF, RNAi 宿主: 小鼠 Monoclonal 4F11 unconjugated

anti-Cyclin-Dependent Kinase 4 (CDK4) (AA 273-305), (C-Term) antibody
CDK4 适用: 人 WB, IF, FACS, IHC (p) 宿主: 兔 Polyclonal RB03154 unconjugated

anti-Cyclin-Dependent Kinase 4 (CDK4) (AA 6-295) antibody
CDK4 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated

anti-Cyclin-Dependent Kinase 4 (CDK4) (AA 201-303) antibody
CDK4 适用: 人, 小鼠, 大鼠 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Cyclin-Dependent Kinase 4 (CDK4) (AA 273-305) antibody
CDK4 适用: 人 WB, ELISA, IHC, IF, FACS 宿主: 兔 Polyclonal unconjugated

anti-Cyclin-Dependent Kinase 4 (CDK4) (AA 241-303) antibody
CDK4 适用: 人, 小鼠, 大鼠, 绵羊 WB, ELISA, FACS, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Cyclin-Dependent Kinase 4 (CDK4) (AA 272-303), (C-Term) antibody
CDK4 适用: 大鼠 WB, IF, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-Cyclin-Dependent Kinase 4 (CDK4) (AA 272-303) antibody
CDK4 适用: 人, 大鼠 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated

应用备注

Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
1. Demetrick, D. J., Zhang, H., Beach, D. H. : Chromosomal mapping of human CDK2, CDK4, and CDK5 cell cycle kinase genes. Cytogenet. Cell Genet. 66: 72-74, 1994. 2. Modiano, J. F., Mayor, J., Ball, C., Fuentes, M. K., Linthicum, D. S. : CDK4 expression and activity are required for cytokine responsiveness in T cells. J. Immun. 165: 6693-6702, 2000.e for human proliferating nuclear antigen/cyclin by in situ hybridization. Hum. Genet. 86: 84-86, 1990.

说明

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

限制

仅限研究用
状态

Lyophilized

溶解方式

Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

浓度

500 μg/mL

缓冲液

Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

储存液

Thimerosal (Merthiolate), Sodium azide

注意事项

This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

注意事项

Avoid repeated freezing and thawing.

储存条件

4 °C,-20 °C

储存方法

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

有效期

12 months
Ren, Huang, Xu, Yang, Yang, Hu: "Isoflavone lupiwighteone induces cytotoxic, apoptotic, and antiangiogenic activities in DU-145 prostate cancer cells." in: Anti-cancer drugs, Vol. 26, Issue 6, pp. 599-611, (2015) (PubMed).

Zhou, Lu, Liu, Guo, Liu, Zhou, Yang, Mi, Xu: "Platycodin D induces tumor growth arrest by activating FOXO3a expression in prostate cancer in vitro and in vivo." in: Current cancer drug targets, Vol. 14, Issue 9, pp. 860-71, (2015) (PubMed).

Zhang, Zhao, Huang, Meng, Bian, Li: "Melittin restores PTEN expression by down-regulating HDAC2 in human hepatocelluar carcinoma HepG2 cells." in: PLoS ONE, Vol. 9, Issue 5, pp. e95520, (2014) (PubMed).

Wang, Huang, Han, Xue, Li, Liu, Shan, Wang, Li, Li: "The effects of artemisinin on the proliferation and apoptosis of vascular smooth muscle cells of rats." in: Cell biochemistry and function, Vol. 32, Issue 2, pp. 201-8, (2014) (PubMed).

Li, Xu, Chu, Gao, Wang, Nie, Yang, Lv: "Molecular mechanism of inhibitory effects of CD59 gene on atherosclerosis in ApoE (-/-) mice." in: Immunology letters, Vol. 156, Issue 1-2, pp. 68-81, (2013) (PubMed).

Cai, Xu, Zhang: "Bone marrow stromal cells induce cell cycle arrest in reactive astrocytes in vitro." in: Neuroscience letters, Vol. 522, Issue 1, pp. 62-6, (2012) (PubMed).

Li, Xu, Li, Zou, Saimaiti, Simayi, Wang, Zhang, Yuan, Zhang: "Stat6 activity-related Th2 cytokine profile and tumor growth advantage of human colorectal cancer cells in vitro and in vivo." in: Cellular signalling, Vol. 24, Issue 3, pp. 718-25, (2012) (PubMed).

Zhang, Ge, Sun, Cao, Wu, Guo, Wang: "Effect of bone morphogenetic protein-2 on proliferation and apoptosis of gastric cancer cells." in: International journal of medical sciences, Vol. 9, Issue 2, pp. 184-92, (2012) (PubMed).

Liu, Dai, Lu, Wei, Ha, Rong, Mu, You, Li, Guo: "LYG-202 inhibits the proliferation of human colorectal carcinoma HCT-116 cells through induction of G1/S cell cycle arrest and apoptosis via p53 and p21(WAF1/Cip1) expression." in: Biochemistry and cell biology = Biochimie et biologie cellulaire, Vol. 89, Issue 3, pp. 287-98, (2011) (PubMed).

Han, Liu: "EGR-1 decoy ODNs inhibit vascular smooth muscle cell proliferation and neointimal hyperplasia of balloon-injured arteries in rat." in: Life sciences, Vol. 86, Issue 7-8, pp. 234-43, (2010) (PubMed).
抗原

CDK4 (Cyclin-Dependent Kinase 4 (CDK4))

别名

CDK4

背景

Synonyms: Cyclin-dependent kinase 4,2.7.11.22,CRK3,Cell division protein kinase 4,PSK-J3,Cdk4,Crk3,
Tissue Specificity: Expressed (at protein level) in a wide range of tissues with highest levels in brain, spinal cord, testis, pancreas, heart, spleen and mammary glands. Moderate levels found in thymus, ovary and small intestine. Not detected in salivary gland, muscle or liver. Also expressed in cell lines of myeloid, fibroblast and epithelial origin. Not detected in most lymphoid cell lines. .
Background: Cyclin-dependent kinase-4 (CDK4) is a protein-serine kinase involved in the cell cycle. Human cell division is regulated primarily at the G1-to-S or the G2-to-M boundaries within the cell cycle. The complexes formed by CDK4 and the D-type cyclins are involved in the control of cell proliferation during the G1 phase. CDK4 is inhibited by p16, also known as cyclin-dependent kinase inhibitor-2. CDK4 is mapped to 12q14. CDK4 expression and activity are required for cytokine responsiveness in T cells.
Sequence Similarities: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.

分子量

34 kDa

UniProt

P30285

途径

Cell Division Cycle, Mitotic G1-G1/S Phases, Regulation of Cell Size