Lamin B1 抗体 (AA 266-583)
(15 references)-
- 抗原 See all Lamin B1 (LMNB1) 抗体
- Lamin B1 (LMNB1)
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抗原表位
- AA 266-583
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适用
- 人, 小鼠, 大鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This Lamin B1 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)
- 原理
- Anti-Lamin B1/LMNB1 Antibody Picoband®
- 交叉反应 （详细）
- No cross-reactivity with other proteins
- 产品特性
- Anti-Lamin B1/LMNB1 Antibody Picoband® (ABIN3042505). Tested in Flow Cytometry, IP, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- 纯化方法
- Immunogen affinity purified.
- 免疫原
- E.coli-derived human Lamin B1 recombinant protein (Position: Q266-C583). Human Lamin B1 shares 95.9% and 95% amino acid (aa) sequence identity with mouse and rat Lamin B1, respectively.
- 亚型
- IgG
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anti-Lamin B1 (LMNB1) (AA 52-233) antibody
LMNB1 适用: 人 WB 宿主: 小鼠 Monoclonal unconjugated
anti-Lamin B1 (LMNB1) (AA 107-186) antibodyLMNB1 适用: 人 WB, ELISA, IF, IHC (p) 宿主: 小鼠 Monoclonal 4E4 unconjugated
anti-Lamin B1 (LMNB1) (AA 107-186) antibodyLMNB1 适用: 人 WB, ELISA, IP, IF 宿主: 小鼠 Monoclonal 4B10 unconjugated
anti-Lamin B1 (LMNB1) (AA 570-586), (C-Term) antibodyLMNB1 适用: 人, 小鼠, 大鼠 WB, IHC, IF, ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Lamin B1 (LMNB1) (AA 266-583) antibodyLMNB1 适用: 人, 小鼠, 大鼠 WB, IHC (p), ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Lamin B1 (LMNB1) (AA 570-586) antibodyLMNB1 适用: 人, 大鼠 WB, IHC (p), ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Lamin B1 (LMNB1) antibodyKO Validated LMNB1 适用: 人 WB, IP, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Lamin B1 (LMNB1) antibodyLMNB1 适用: 人, 小鼠, 大鼠 WB, IP, IF, IHC (p) 宿主: 小鼠 Monoclonal 4K2 unconjugated
anti-Lamin B1 (LMNB1) (AA 361-460) antibodyLMNB1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p), FACS, IHC (fro), IF (cc), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-Lamin B1 (LMNB1) antibodyLMNB1 适用: 人 WB, ELISA, IP 宿主: 小鼠 Monoclonal unconjugated
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- 应用备注
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Western blot, 0.1-0.5 μg/mL
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL
Immunocytochemistry/Immunofluorescence, 5 μg/mL
Immunoprecipitation, 0.5-2 μg/mL
Flow Cytometry (Fixed), 1-3 μg/1x106 cells
1. Pollard, K. M., Chan, E. K., Grant, B. J., Sullivan, K. E., Tan, E. M., Glass, C. A. : In vitro posttranslational modification of lamin B clones from a human T-cell line. Molec. Cell. Biol. 10: 2164-2175, 1990. 2. Tsai, M.-Y., Wang, S., Heidinger, J. M., Shumaker, D. K., Adam, S. A., Goldman, R. D., Zheng, Y. : A mitotic lamin B matrix induced by RanGTP required for spindle assembly. Science 311: 1887-1893, 2006. - 说明
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
- 浓度
- 500 μg/mL
- 缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C,-20 °C
- 储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- 抗原
- Lamin B1 (LMNB1)
- 别名
- LMNB1 (LMNB1 产品)
- 背景
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Synonyms: Lamin-B1,LMNB1,LMN2, LMNB,
Tissue Specificity: Expressed in bone marrow and in tissues that are prone to exposure to microorganism. High expression is found in bone marrow as well as in uterus, prostate, salivary gland, stomach, appendix, colon, trachea and lung. Not found in the small intestine or peripheral blood leukocytes. .
Background: Lamin-B1 is a protein that in humans is encoded by the LMNB1 gene. The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. This gene encodes one of the two B type proteins, B1.
Sequence Similarities: Belongs to the intermediate filament family.
- 分子量
- 67 kDa
- 基因ID
- 4001
- UniProt
- P20700
- 途径
- Apoptosis, Caspase Cascade in Apoptosis
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