GRK6 抗体 (C-Term)
(1 reference)
(1 validation)-
- 抗原 See all GRK6 抗体
- GRK6 (G Protein-Coupled Receptor Kinase 6 (GRK6))
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抗原表位
- AA 382-417, C-Term
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适用
- 人, 大鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This GRK6 antibody is un-conjugated
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应用范围
- Western Blotting (WB)
- 原理
- Rabbit IgG polyclonal antibody for G protein-coupled receptor kinase 6(GRK6) detection. Tested with WB in Human,Rat.
- 序列
- QSPFQQRKKK IKREEVERLV KEVPEEYSER FSPQAR
- 交叉反应 （详细）
- No cross reactivity with other proteins.
- 产品特性
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Rabbit IgG polyclonal antibody for G protein-coupled receptor kinase 6(GRK6) detection. Tested with WB in Human,Rat.
Gene Name: G protein-coupled receptor kinase 6
Protein Name: G protein-coupled receptor kinase 6 - 纯化方法
- Immunogen affinity purified.
- 免疫原
- A synthetic peptide corresponding to a sequence at the C-terminus of human GRK6 (382-417aa QSPFQQRKKKIKREEVERLVKEVPEEYSERFSPQAR), different from the related mouse sequence by four amino acids, and from the related rat sequence by three amino acids.
- 亚型
- IgG
- Top Product
- Discover our top product GRK6 Primary Antibody
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anti-G Protein-Coupled Receptor Kinase 6 (GRK6) (AA 71-170) antibody
GRK6 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 2G1 unconjugated
anti-G Protein-Coupled Receptor Kinase 6 (GRK6) (AA 71-170) antibodyGRK6 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 8D4 unconjugated
anti-G Protein-Coupled Receptor Kinase 6 (GRK6) (AA 71-170) antibodyGRK6 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 2C3 unconjugated
anti-G Protein-Coupled Receptor Kinase 6 (GRK6) (N-Term) antibodyGRK6 适用: 人 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-G Protein-Coupled Receptor Kinase 6 (GRK6) (AA 1-170) antibodyGRK6 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-G Protein-Coupled Receptor Kinase 6 (GRK6) (AA 1-170) antibodyGRK6 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-G Protein-Coupled Receptor Kinase 6 (GRK6) (AA 1-589) antibodyGRK6 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-G Protein-Coupled Receptor Kinase 6 (GRK6) (N-Term) antibodyGRK6 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-G Protein-Coupled Receptor Kinase 6 (GRK6) (AA 186-448) antibodyGRK6 适用: 人 WB, IHC, ICC 宿主: 兔 Polyclonal unconjugated
anti-G Protein-Coupled Receptor Kinase 6 (GRK6) (N-Term) antibodyGRK6 适用: 人, 小鼠, 大鼠 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat
Notes: Tested Species: Species with positive results.
Other applications have not been tested. Optimal dilutions should be determined by end users. - 说明
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB.
- 限制
- 仅限研究用
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- by
- Institut für Molekulare Zellbiologie, Universitätsklinikum Jena
- No.
- #103590
- 日期
- 2019.01.31
- 抗原
- GRK6
- Lot Number
- 0971512Da9609109
- Method validated
- Western Blotting
- Positive Control
HEK293 cells transiently transfected with pcDNA3 vectors containing GRK6 transcript variant 1 (NM_001004106.3), transcript variant 2 (NM_002082.3), transcript variant 3 (NM_001004105.2), or transcript variant 4 (NM_001364164.1)
- Negative Control
HEK293 cells transiently transfected with empty pcDNA3 vector
- Notes
The GRK6 antibody ABIN3042435 is able to detect all four tested GRK6 isoforms, but gives strong background-signals around 55kDa.
- Primary Antibody
- ABIN3042435
- Secondary Antibody
- goat-anti-rabbit Peroxidase-labeled antibody (SeraCare, 5220-0336)
- Full Protocol
- Grow 7.5x105 HEK293 cells in DMEM-medium (Sigma-Aldrich, D6429)) supplemented with 10% Fetal Bovine serum (Sigma-Aldrich, F7524) and 1% penicillin-streptomycin (Sigma-Aldrich, P0781), at 37°C and 5% CO2 in 2ml on a 6-well-dish (Greiner Bio-One) ON.
- Transfect cells with 2μg of respective pcDNA3 construct using self-prepared PEI transfection reagent.
- After 24h lyse the cells in 250μl per well cold RIPA lysis buffer (1 % NP-40, 1mM EDTA, 50mM Tris-HCl pH7.4, 150mM NaCl, 0.25 % Sodium-deoxycholate, PhosSTOP tablet (Roche, 04906845001) and cOmplete tablet (Roche, 04693132001) diluted in RIPA following the manufacturer´s instructions.
- Denature the cleared lysate of total protein for 5min at 95°C in 50μl of 6x SDS sample buffer and subsequently separate 4μl of each sample on a 10% polyacrylamide gel.
- Transfer proteins onto nitrocellulose membrane (Biostep 01-14-101) with a Tank Blotting System at 10V ON.
- Block the membrane with 1x Casein Blocking Buffer (Sigma-Aldrich, B6429) for 1h at RT with gentle shaking.
- Wash membrane 3x for 10min with TBST.
- Cut the membrane and incubate fragments separately with primary
- rabbit anti-GRK6 antibody (ABIN3042435, antibodies-online, lot 0971512Da9609109) diluted 1:1000 in 5% BSA-TBST at 4°C ON.
- rabbit anti-GRK6 antibody (Cell Signaling Technology, 5878, lot 1) diluted 1:1000 in 5% BSA-TBST at 4°C ON.
- rabbit anti-Vinculin antibody (Biozol, BZL03106, lot 0401) diluted 1:1000 in 5% BSA-TBST at 4°C ON.
- Wash membrane 3x for 10min with TBST.
- Incubate membrane with secondary goat-anti-rabbit Peroxidase-labeled antibody (SeraCare, 5220-0336) diluted 1:10000 in 1x Casein Blocking Buffer) for 1h at RT with gentle shaking.
- Wash membrane 3x for 10min with TBST.
- Reveal protein bands using Western Lightning Plus ECL reagent (Perkin Elmer, NEL103001EA, Luminol reagent Lot 275-17431, Oxidizing reagent, lot 265-17431) on a LAS-4000 Luminescence Imager (Fujifilm), exposure for 15 and 90 seconds.
- Experimental Notes
If the detection of the smallest isoform (GRK6-4, transcript variant 4, NM_001364164.1) is required, ABIN3042435 is a good choice, as this isoform is not detected by the alternative GRK6 antibody tested in parallel. Nevertheless, the strong background signal around 55kDa produced by ABIN3042435 diminishes its usefulness.
生效 #103590 (Western Blotting)!['独立验证'标志]( "成功验证")
!['独立验证'标志]( "成功验证")
Validation Images![Western blot analysis of HEK293 cell lysates subsequently to transient transfection with pcDNA3 constructs containing no insert (EV), GRK6 transcript variant 1, NM_001004106.3, transcript variant 2, NM_002082.3, transcript variant 3, NM_001004105.2, or transcript variant 4, NM_001364164.1 as indicated. Equal amounts of the lysates were loaded twice on the same gel and after blotting, the membrane was cut and incubated with ABIN3042435, a reference anti-GRK6 antibody (GRK6), and Vinculin loading control antibody (Vinculin) in parallel. Exposure for the upper panel was 15 seconds and for the lower 90 seconds.]()
Western blot analysis of HEK293 cell lysates subsequently to transient transfection with pcDNA3 constructs containing no insert (EV), GRK6 transcript variant 1, NM_001004106.3, transcript variant 2, NM_002082.3, transcript variant 3, NM_001004105.2, or transcript variant 4, NM_001364164.1 as indicated. Equal amounts of the lysates were loaded twice on the same gel and after blotting, the membrane was cut and incubated with ABIN3042435, a reference anti-GRK6 antibody (GRK6), and Vinculin loading control antibody (Vinculin) in parallel. Exposure for the upper panel was 15 seconds and for the lower 90 seconds.
Full Methods -
- 状态
- Lyophilized
- 溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
- 浓度
- 500 μg/mL
- 缓冲液
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C/-20 °C
- 储存方法
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At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
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: "Suitability of GRK Antibodies for Individual Detection and Quantification of GRK Isoforms in Western Blots." in: International journal of molecular sciences, Vol. 23, Issue 3, (2022) (PubMed).
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: "Suitability of GRK Antibodies for Individual Detection and Quantification of GRK Isoforms in Western Blots." in: International journal of molecular sciences, Vol. 23, Issue 3, (2022) (PubMed).
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- 抗原
- GRK6 (G Protein-Coupled Receptor Kinase 6 (GRK6))
- 别名
- GRK6 (GRK6 产品)
- 背景
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G protein-coupled receptor kinase 6 is an enzyme that in humans is encoded by the GRK6 gene. It is mapped to 5q35. This gene encodes a member of the guanine nucleotide-binding protein (G protein)-coupled receptor kinase subfamily of the Ser/Thr protein kinase family. The protein phosphorylates the activated forms of G protein-coupled receptors thus initiating their deactivation. Several transcript variants encoding different isoforms have been described for this gene. Also, GRK6 appears to be involved in responses to morphine.
Synonyms: FLJ32135 antibody|G protein coupled receptor kinase 6 antibody|G protein coupled receptor kinase GRK6 antibody|G protein-coupled receptor kinase 6 antibody|G protein-coupled receptor kinase GRK6 antibody|Gprk6 antibody|Grk6 antibody|GRK6_HUMAN antibody - 基因ID
- 2870
- UniProt
- P43250
- 途径
- Myometrial Relaxation and Contraction, Regulation of G-Protein Coupled Receptor Protein Signaling, CXCR4-mediated Signaling Events, Negative Regulation of Transporter Activity
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