电话:
+86 (0512) 65829739
传真:
+86 (010) 6788 5057
电子邮件:
orders@antibodies-online.cn

GRK6 抗体 (C-Term)

GRK6 适用: 人, 大鼠 WB 宿主: 兔 Polyclonal unconjugated
产品编号 ABIN3042435
发货至: 中国
  • 抗原 See all GRK6 抗体
    GRK6 (G Protein-Coupled Receptor Kinase 6 (GRK6))
    抗原表位
    • 8
    • 5
    • 4
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    AA 382-417, C-Term
    适用
    • 36
    • 23
    • 14
    • 2
    • 1
    • 1
    • 1
    • 1
    人, 大鼠
    宿主
    • 31
    • 5
    克隆类型
    • 32
    • 4
    多克隆
    标记
    • 24
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This GRK6 antibody is un-conjugated
    应用范围
    • 28
    • 15
    • 13
    • 5
    • 3
    • 3
    • 2
    Western Blotting (WB)
    原理
    Anti-GRK6 Antibody Picoband®
    序列
    QSPFQQRKKK IKREEVERLV KEVPEEYSER FSPQAR
    交叉反应 (详细)
    No cross-reactivity with other proteins
    产品特性
    Anti-GRK6 Antibody Picoband® (ABIN3042435). Tested in WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
    纯化方法
    Immunogen affinity purified.
    免疫原
    A synthetic peptide corresponding to a sequence at the C-terminus of human GRK6, different from the related mouse sequence by four amino acids, and from the related rat sequence by three amino acids.
    亚型
    IgG
    Top Product
    Discover our top product GRK6 Primary Antibody
  • 应用备注
    Western blot, 0.1-0.5 μg/mL, Human, Rat
    1. "Entrez Gene: GRK6 G protein-coupled receptor kinase 6". 2. Haribabu B, Snyderman R (Nov 1993). "Identification of additional members of human G-protein-coupled receptor kinase multigene family". Proc Natl Acad Sci U S A 90 (20): 9398-402. 3. Raehal KM, Schmid CL, Medvedev IO, Gainetdinov RR, Premont RT, Bohn LM (June 2009). "Morphine-Induced Physiological and Behavioral Responses in Mice Lacking G Protein-Coupled Receptor Kinase 6". Drug and Alcohol Dependence 104 (3): 187-96. 
    说明

    Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

    限制
    仅限研究用
  • 生效 #103590 (Western Blotting)
    '独立验证'标志
    by
    Institut für Molekulare Zellbiologie, Universitätsklinikum Jena
    No.
    #103590
    日期
    2019.01.31
    抗原
    GRK6
    Lot Number
    0971512Da9609109
    Method validated
    Western Blotting
    Positive Control

    HEK293 cells transiently transfected with pcDNA3 vectors containing GRK6 transcript variant 1 (NM_001004106.3), transcript variant 2 (NM_002082.3), transcript variant 3 (NM_001004105.2), or transcript variant 4 (NM_001364164.1)

    Negative Control

    HEK293 cells transiently transfected with empty pcDNA3 vector

    Notes

    The GRK6 antibody ABIN3042435 is able to detect all four tested GRK6 isoforms, but gives strong background-signals around 55kDa.

    '独立验证'标志
    Validation Images
    Full Methods
    Primary Antibody
    ABIN3042435
    Secondary Antibody
    goat-anti-rabbit Peroxidase-labeled antibody (SeraCare, 5220-0336)
    Full Protocol
    • Grow 7.5x105 HEK293 cells in DMEM-medium (Sigma-Aldrich, D6429)) supplemented with 10% Fetal Bovine serum (Sigma-Aldrich, F7524) and 1% penicillin-streptomycin (Sigma-Aldrich, P0781), at 37°C and 5% CO2 in 2ml on a 6-well-dish (Greiner Bio-One) ON.
    • Transfect cells with 2μg of respective pcDNA3 construct using self-prepared PEI transfection reagent.
    • After 24h lyse the cells in 250μl per well cold RIPA lysis buffer (1 % NP-40, 1mM EDTA, 50mM Tris-HCl pH7.4, 150mM NaCl, 0.25 % Sodium-deoxycholate, PhosSTOP tablet (Roche, 04906845001) and cOmplete tablet (Roche, 04693132001) diluted in RIPA following the manufacturer´s instructions.
    • Denature the cleared lysate of total protein for 5min at 95°C in 50μl of 6x SDS sample buffer and subsequently separate 4μl of each sample on a 10% polyacrylamide gel.
    • Transfer proteins onto nitrocellulose membrane (Biostep 01-14-101) with a Tank Blotting System at 10V ON.
    • Block the membrane with 1x Casein Blocking Buffer (Sigma-Aldrich, B6429) for 1h at RT with gentle shaking.
    • Wash membrane 3x for 10min with TBST.
    • Cut the membrane and incubate fragments separately with primary
      • rabbit anti-GRK6 antibody (ABIN3042435, antibodies-online, lot 0971512Da9609109) diluted 1:1000 in 5% BSA-TBST at 4°C ON.
      • rabbit anti-GRK6 antibody (Cell Signaling Technology, 5878, lot 1) diluted 1:1000 in 5% BSA-TBST at 4°C ON.
      • rabbit anti-Vinculin antibody (Biozol, BZL03106, lot 0401) diluted 1:1000 in 5% BSA-TBST at 4°C ON.
    • Wash membrane 3x for 10min with TBST.
    • Incubate membrane with secondary goat-anti-rabbit Peroxidase-labeled antibody (SeraCare, 5220-0336) diluted 1:10000 in 1x Casein Blocking Buffer) for 1h at RT with gentle shaking.
    • Wash membrane 3x for 10min with TBST.
    • Reveal protein bands using Western Lightning Plus ECL reagent (Perkin Elmer, NEL103001EA, Luminol reagent Lot 275-17431, Oxidizing reagent, lot 265-17431) on a LAS-4000 Luminescence Imager (Fujifilm), exposure for 15 and 90 seconds.
    Experimental Notes

    If the detection of the smallest isoform (GRK6-4, transcript variant 4, NM_001364164.1) is required, ABIN3042435 is a good choice, as this isoform is not detected by the alternative GRK6 antibody tested in parallel. Nevertheless, the strong background signal around 55kDa produced by ABIN3042435 diminishes its usefulness.

  • 状态
    Lyophilized
    溶解方式
    Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
    浓度
    500 μg/mL
    缓冲液
    Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    注意事项
    Avoid repeated freezing and thawing.
    储存条件
    4 °C,-20 °C
    储存方法
    Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
    It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
  • Reichel, Weitzel, Klement, Hoffmann, Drube: "Suitability of GRK Antibodies for Individual Detection and Quantification of GRK Isoforms in Western Blots." in: International journal of molecular sciences, Vol. 23, Issue 3, (2022) (PubMed).

  • 抗原
    GRK6 (G Protein-Coupled Receptor Kinase 6 (GRK6))
    别名
    GRK6 (GRK6 产品)
    背景

    Synonyms: G protein-coupled receptor kinase 6,2.7.11.16,G protein-coupled receptor kinase GRK6,GRK6,GPRK6,

    Tissue Specificity: Widely expressed. .

    Background: G protein-coupled receptor kinase 6?is an?enzyme?that in humans is encoded by the?GRK6?gene. It is mapped to 5q35. This gene encodes a member of the guanine nucleotide-binding protein (G protein)-coupled receptor kinase subfamily of the Ser/Thr protein kinase family. The protein phosphorylates the activated forms of G protein-coupled receptors thus initiating their deactivation. Several transcript variants encoding different isoforms have been described for this gene. Also, GRK6 appears to be involved in responses to?morphine.

    分子量
    66 kDa
    基因ID
    2870
    UniProt
    P43250
    途径
    Myometrial Relaxation and Contraction, Regulation of G-Protein Coupled Receptor Protein Signaling, CXCR4-mediated Signaling Events, Negative Regulation of Transporter Activity
You are here:
Chat with us!