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GCLC 抗体 (AA 437-637)

This anti-GCLC antibody is a 兔 多克隆 antibody detecting GCLC in WB, IHC 和 FACS. Suitable for 人, 小鼠 和 大鼠. This Primary Antibody has been cited in 2+ publications.
产品编号 ABIN3042420
发货至: 中国

Quick Overview for GCLC 抗体 (AA 437-637) (ABIN3042420)

抗原

See all GCLC 抗体
GCLC (Glutamate-Cysteine Ligase, Catalytic Subunit (GCLC))

适用

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人, 小鼠, 大鼠

宿主

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克隆类型

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多克隆

标记

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This GCLC antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)
  • 抗原表位

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    AA 437-637

    原理

    Anti-GCLC Antibody Picoband®

    交叉反应 (详细)

    No cross-reactivity with other proteins

    产品特性

    Anti-GCLC Antibody Picoband® (ABIN3042420). Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

    纯化方法

    Immunogen affinity purified.

    免疫原

    E.coli-derived human GCLC recombinant protein (Position: E437-N637). Human GCLC shares 94% amino acid (aa) sequence identity with both mouse and rat GCLC.

    亚型

    IgG
  • 应用备注

    Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
    Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human
    Flow Cytometry(Fixed), 1-3 μg/1x106 cells, Human
    1. Gipp, J. J., Chang, C., Mulcahy, R. T.Cloning and nucleotide sequence of a full-length cDNA for human liver gamma-glutamylcysteine synthetase.Biochem. Biophys. Res. Commun. 185: 29-35, 1992. 2. Sierra-Rivera, E., Summar, M. L., Dasouki, M., Krishnamani, M. R. S., Phillips, J. A., Freeman, M. L.Assignment of the gene (GLCLC) that encodes the heavy subunit of gamma-glutamylcysteine synthetase to human chromosome 6.Cytogenet. Cell Genet. 70: 278-279, 1995.

    说明

    Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

    限制

    仅限研究用
  • 状态

    Lyophilized

    溶解方式

    Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

    浓度

    500 μg/mL

    缓冲液

    Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

    注意事项

    Avoid repeated freezing and thawing.

    储存条件

    4 °C,-20 °C

    储存方法

    Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
    It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
  • Ni, Wang, Qiu, Pang, Song, Guo: "Bone marrow mesenchymal stem cells protect against bleomycin-induced pulmonary fibrosis in rat by activating Nrf2 signaling." in: International journal of clinical and experimental pathology, Vol. 8, Issue 7, pp. 7752-61, (2016) (PubMed).

    Jin, Li, Zhang, Wu, Feng, Zhang, Shen: "Electroacupuncture alleviates cerebral ischemia and reperfusion injury via modulation of the ERK1/2 signaling pathway." in: Neural regeneration research, Vol. 11, Issue 7, pp. 1090-8, (2016) (PubMed).

  • 抗原

    GCLC (Glutamate-Cysteine Ligase, Catalytic Subunit (GCLC))

    别名

    GCLC

    背景

    Synonyms: Glutamate--cysteine ligase catalytic subunit,6.3.2.2,GCS heavy chain,Gamma-ECS,Gamma-glutamylcysteine synthetase,GCLC,GLCL, GLCLC,

    Tissue Specificity: T-cells and endothelial cells.

    Background: GCLC, also named Glutamate--cysteine ligase catalytic subunit, is an enzyme that in humans is encoded by the GCLC gene. Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. The gene encoding the catalytic subunit encodes a protein of 367 amino acids with a calculated molecular weight of 72.773 kDa and maps to chromosome 6p12.1. Deficiency of gamma-glutamylcysteine synthetase in human is associated with enzymopathic hemolytic anemia.

    Sequence Similarities: Belongs to the glutamate--cysteine ligase type 3 family.

    分子量

    72 kDa

    基因ID

    2729

    UniProt

    P48506

    途径

    Cell RedoxHomeostasis
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