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CD20 抗体 (AA 1-261)

MS4A1 适用: 人 FACS, WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
产品编号 ABIN3042388
发货至: 中国
  • 抗原 See all CD20 (MS4A1) 抗体
    CD20 (MS4A1) (Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1))
    抗原表位
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    AA 1-261
    适用
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    宿主
    • 191
    • 95
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    克隆类型
    • 209
    • 79
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    多克隆
    标记
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    This CD20 antibody is un-conjugated
    应用范围
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    Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    原理
    Rabbit IgG polyclonal antibody for B-lymphocyte antigen CD20(MS4A1) detection. Tested with WB, IHC-P, FCM in Human.
    交叉反应 (详细)
    No cross reactivity with other proteins.
    产品特性
    Rabbit IgG polyclonal antibody for B-lymphocyte antigen CD20(MS4A1) detection. Tested with WB, IHC-P, FCM in Human.
    Gene Name: membrane-spanning 4-domains, subfamily A, member 1
    Protein Name: B-lymphocyte antigen CD20
    纯化方法
    Immunogen affinity purified.
    免疫原
    E.coli-derived human CD20 recombinant protein (Position: M1-D261). Human CD20 shares 75% amino acid (aa) sequence identity with mouse CD20.
    亚型
    IgG
    Top Product
    Discover our top product MS4A1 Primary Antibody
  • 应用备注
    WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, The detection limit for CD20 is approximately 0.25 ng/lane under reducing conditions.
    IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
    Flow Cytometry: Concentration:1-3 μg/1x106 cells, Tested Species: Human
    Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.
    说明

    Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式
    Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
    浓度
    500 μg/mL
    缓冲液
    Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    注意事项
    Avoid repeated freezing and thawing.
    储存条件
    4 °C/-20 °C
    储存方法
    At -20°C for one year. After reconstitution, at 4°C for one month.
    It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
  • Ni, Chen, Zeng, Pei, Du, Tang, Wang, Hu, Zhu, He, Wei, Wang, Ning, Wang, Wang, Ma, Chen, Sun, Tang, Wang, Wang: "In-cell infection: a novel pathway for Epstein-Barr virus infection mediated by cell-in-cell structures." in: Cell research, Vol. 25, Issue 7, pp. 785-800, (2016) (PubMed).

    Yan, Fan, Bai, Hou, Li, Tong: "Zinc Prevents Abdominal Aortic Aneurysm Formation by Induction of A20-Mediated Suppression of NF-κB Pathway." in: PLoS ONE, Vol. 11, Issue 2, pp. e0148536, (2016) (PubMed).

  • 抗原
    CD20 (MS4A1) (Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1))
    别名
    MS4A1 (MS4A1 产品)
    别名
    B1 antibody, Bp35 antibody, CD20 antibody, CVID5 antibody, LEU-16 antibody, MS4A2 antibody, S7 antibody, AA960661 antibody, Cd20 antibody, Ly-44 antibody, Ms4a2 antibody, MS4A1 antibody, bp35 antibody, cd20 antibody, ms4a2 antibody, leu-16 antibody, ms4a4c antibody, cd20-like antibody, membrane spanning 4-domains A1 antibody, membrane-spanning 4-domains, subfamily A, member 1 antibody, B-lymphocyte antigen CD20 antibody, MS4A1 antibody, Ms4a1 antibody, ms4a1 antibody, LOC101694281 antibody
    背景
    CD20, also known as MS4A1, is an activated-glycosylated phosphoprotein expressed on the surface of all B-cells beginning at the pro-B phase (CD45R+, CD117+) and progressively increasing in concentration until maturity. It is mapped to 11q12.2. This gene encodes a member of the membrane-spanning 4A gene family. The function of CD20 is to enable optimal B-cell immune response, specifically against T-independent antigens. It is suspected that CD20 acts as a calcium channel in the cell membrane. What's more, this protein may be involved in the regulation of B-cell activation and proliferation.

    Synonyms: APY antibody|ATOPY antibody|B lymphocyte antigen CD20 antibody|B Lymphocyte Cell Surface Antigen B1 antibody|B-lymphocyte antigen CD20 antibody|B-lymphocyte cell-surface antigen B1 antibody|B-lymphocyte surface antigen B1 antibody|B1 antibody|Bp 35 antibody|Bp35 antibody|CD 20 antibody|CD20 antibody|CD20 antigen antibody|CD20 receptor antibody|CD20_HUMAN antibody|Fc epsilon receptor I beta chain antibody|Fc Fragment of IgE high affinity I receptor for beta polypeptide antibody|FCER1B antibody|FCERI antibody|High affinity immunoglobulin epsilon receptor subunit beta antibody|IgE Fc receptor subunit beta antibody|IGEL antibody|IGER antibody|IGHER antibody|LEU16 antibody|LEU16 antibody|leukocyte surface antigen Leu 16 antibody|Leukocyte surface antigen Leu-16 antibody|Ly44 antibody|Membrane spanning 4 domains subfamily A member 2 antibody|Membrane-spanning 4-domains subfamily A member 1 antibody|MS4A1 antibody
    基因ID
    931
    UniProt
    P11836
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