电话:
+86 (0512) 65829739
传真:
+86 (010) 6788 5057
电子邮件:
orders@antibodies-online.cn

CD20 抗体 (AA 1-261)

MS4A1 适用: 人 FACS, WB, IF, IHC, IHC (fro), ICC 宿主: 兔 Polyclonal unconjugated
产品编号 ABIN3042388
发货至: 中国
  • 抗原 See all CD20 (MS4A1) 抗体
    CD20 (MS4A1) (Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1))
    抗原表位
    • 16
    • 15
    • 13
    • 8
    • 7
    • 6
    • 4
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 1-261
    适用
    • 229
    • 61
    • 26
    • 18
    • 17
    • 16
    • 8
    • 6
    • 2
    • 2
    • 2
    • 1
    • 1
    宿主
    • 155
    • 100
    • 6
    • 3
    • 2
    • 1
    克隆类型
    • 181
    • 75
    • 8
    多克隆
    标记
    • 113
    • 18
    • 13
    • 12
    • 7
    • 6
    • 6
    • 6
    • 6
    • 6
    • 6
    • 5
    • 5
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This CD20 antibody is un-conjugated
    应用范围
    • 186
    • 93
    • 80
    • 52
    • 50
    • 31
    • 28
    • 16
    • 15
    • 15
    • 13
    • 12
    • 11
    • 6
    • 5
    • 5
    • 5
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Flow Cytometry (FACS), Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC)
    原理
    Anti-CD20/MS4A1 Antibody Picoband®
    交叉反应 (详细)
    No cross-reactivity with other proteins
    产品特性
    Anti-CD20/MS4A1 Antibody Picoband® (ABIN3042388). Tested in Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
    纯化方法
    Immunogen affinity purified.
    免疫原
    E.coli-derived human CD20 recombinant protein (Position: M1-D261). Human CD20 shares 75% amino acid (aa) sequence identity with mouse CD20.
    亚型
    IgG
    Top Product
    Discover our top product MS4A1 Primary Antibody
  • 应用备注
    Western blot, 0.1-0.5 μg/mL
    Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL
    Immunohistochemistry (Frozen Section), 0.5-1 μg/mL
    Immunocytochemistry, 0.5-1 μg/mL
    Immunofluorescence, 2 μg/mL, Human
    Flow Cytometry (Fixed), 1-3 μg/1x106cells
    1. Cragg MS, Walshe CA, Ivanov AO, Glennie MJ (2005). "The biology of CD20 and its potential as a target for mAb therapy". Curr. Dir. Autoimmun. Current ions in Autoimmunity 8: 140-74. 2. Hardy, Richard (2008). "Chapter 7: B Lymphocyte Development and Biology". In Paul, William.Fundamental Immunology (Book) (6th ed.). Philadelphia: Lippincott Williams & Wilkins. pp. 237-269. 3. Kuijpers TW, Bende RJ, Baars PA, Grummels A, Derks IA, Dolman KM, Beaumont T, Tedder TF, van Noesel CJ, Eldering E, van Lier RA (January 2010). "CD20 deficiency in humans results in impaired T cell-independent antibody responses". J. Clin. Invest. 120 (1): 214-22.
    说明

    Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式
    Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
    浓度
    500 μg/mL
    缓冲液
    Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    注意事项
    Avoid repeated freezing and thawing.
    储存条件
    4 °C,-20 °C
    储存方法
    Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
    It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
  • Ni, Chen, Zeng, Pei, Du, Tang, Wang, Hu, Zhu, He, Wei, Wang, Ning, Wang, Wang, Ma, Chen, Sun, Tang, Wang, Wang: "In-cell infection: a novel pathway for Epstein-Barr virus infection mediated by cell-in-cell structures." in: Cell research, Vol. 25, Issue 7, pp. 785-800, (2016) (PubMed).

    Yan, Fan, Bai, Hou, Li, Tong: "Zinc Prevents Abdominal Aortic Aneurysm Formation by Induction of A20-Mediated Suppression of NF-κB Pathway." in: PLoS ONE, Vol. 11, Issue 2, pp. e0148536, (2016) (PubMed).

  • 抗原
    CD20 (MS4A1) (Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1))
    别名
    MS4A1 (MS4A1 产品)
    背景

    Synonyms: B-lymphocyte antigen CD20,B-lymphocyte surface antigen B1,Bp35,Leukocyte surface antigen Leu-16,Membrane-spanning 4-domains subfamily A member 1,CD20,MS4A1,CD20,

    Tissue Specificity: Expressed on B-cells.

    Background: CD20, also known as MS4A1, is an activated-glycosylated phosphoprotein expressed on the surface of all B-cells beginning at the pro-B phase (CD45R+, CD117+) and progressively increasing in concentration until maturity. It is mapped to 11q12.2. This gene encodes a member of the membrane-spanning 4A gene family. The function of CD20 is to enable optimal B-cell immune response, specifically against T-independent antigens. It is suspected that CD20 acts as a calcium channel in the cell membrane. What's more, this protein may be involved in the regulation of B-cell activation and proliferation.

    Sequence Similarities: Belongs to the MS4A family.

    分子量
    35 kDa
    基因ID
    931
    UniProt
    P11836
You are here:
Chat with us!