ZEB2 抗体 (AA 1-200)
Quick Overview for ZEB2 抗体 (AA 1-200) (ABIN3042325)
抗原
See all ZEB2 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 1-200
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原理
- Anti-Smad Interacting Protein 1/ZEB2 Antibody Picoband®
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交叉反应 （详细）
- No cross-reactivity with other proteins
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产品特性
- Anti-Smad Interacting Protein 1/ZEB2 Antibody Picoband® (ABIN3042325). Tested in Flow Cytometry, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human ZEB2 recombinant protein (Position: M1-P200). Human ZEB2 shares 92% amino acid (aa) sequence identity with mouse ZEB2.
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亚型
- IgG
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anti-Zinc Finger E-Box Binding Homeobox 2 (ZEB2) (AA 1115-1214) antibody
ZEB2 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 2D12 unconjugated
anti-Zinc Finger E-Box Binding Homeobox 2 (ZEB2) (Middle Region) antibodyZEB2 适用: 人, 小鼠, 大鼠, Cow, 马, 豚鼠, Pig, 斑马鱼 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Zinc Finger E-Box Binding Homeobox 2 (ZEB2) (AA 1115-1214) antibodyZEB2 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 2H5 unconjugated
anti-Zinc Finger E-Box Binding Homeobox 2 (ZEB2) (N-Term) antibodyZEB2 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-Zinc Finger E-Box Binding Homeobox 2 (ZEB2) (AA 1-350) antibodyZEB2 适用: 人 WB, IHC, IHC (p) 宿主: 小鼠 Monoclonal 15G8 unconjugated
anti-Zinc Finger E-Box Binding Homeobox 2 (ZEB2) (AA 1050-1214) antibodyZEB2 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Zinc Finger E-Box Binding Homeobox 2 (ZEB2) (N-Term) antibodyZEB2 适用: 人, 小鼠, 大鼠, 兔, Cow, 马, 犬, 豚鼠, Pig WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Zinc Finger E-Box Binding Homeobox 2 (ZEB2) (AA 1050-1214) antibodyZEB2 适用: 人 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Zinc Finger E-Box Binding Homeobox 2 (ZEB2) (AA 30-79) antibodyZEB2 适用: 人, 兔, Cow, 豚鼠, 猴, 斑马鱼 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Zinc Finger E-Box Binding Homeobox 2 (ZEB2) (C-Term) antibodyZEB2 适用: 人, 小鼠, 大鼠, 兔, Cow, 马, 猴, 斑马鱼, 小鸡, Bat WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.1-0.5 μg/mL, Human
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Frozen Section), 0.5-1 μg/mL, Human
Immunocytochemistry, 0.5-1 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human1. Beltran, M., Puig, I., Pena, C., Garcia, J. M., Alvarez, A. B., Pena, R., Bonilla, F., Garcia de Herreros, A. A natural antisense transcript regulates Zeb2/Sip1 gene expression during Snail1-induced epithelial-mesenchymal transition. Genes Dev. 22: 756-769, 2008. 2. Nagase, T., Ishikawa, K., Miyajima, N., Tanaka, A., Kotani, H., Nomura, N., Ohara, O. Prediction of the coding sequences of unidentified human genes. IX. The complete sequences of 100 new cDNA clones from brain which can code for large proteins in vitro. DNA Res. 5: 31-39, 1998. 3. Vandewalle, C., Comijn, J., De Craene, B., Vermassen, P., Bruyneel, E., Andersen, H., Tulchinsky, E., Van Roy, F., Berx, G. SIP1/ZEB2 induces EMT by repressing genes of different epithelial cell-cell junctions. Nucleic Acids Res. 33: 6566-6578, 2005. 4. Wakamatsu, N., Yamada, Y., Yamada, K., Ono, T., Nomura, N., Taniguchi, H., Kitoh, H., Mutoh, N., Yamanaka, T., Mushiake, K., Kato, K., Sonta, S., Nagaya, M. Mutations in SIP1, encoding Smad interacting protein-1, cause a form of Hirschsprung disease. Nature Genet. 27: 369-370, 2001. -
说明
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- ZEB2 (Zinc Finger E-Box Binding Homeobox 2 (ZEB2))
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别名
- ZEB2
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背景
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Synonyms: Zinc finger E-box-binding homeobox 2,Smad-interacting protein 1,SMADIP1,Zinc finger homeobox protein 1b,ZEB2,KIAA0569, SIP1, ZFHX1B, ZFX1B,HRIHFB2411,
Tissue Specificity: Expressed in striatal neurons of patients with Huntington disease (at protein level). Brain, kidney, placenta, colon, heart, liver, spleen, skeletal muscle, prostate, thymus and pancreas. Highly expressed in fetal tissue. .
Background: ZEB2 (Zinc finger E-box-binding homeobox2), also known as SIP1 or ZINC FINGER HOMEOBOX 1B (ZFHX1B), is a protein that in humans is encoded by the ZEB2 gene. The ZEB2 gene is a member of the ZEB1/Drosophila Zfh1 family of 2-handed zinc finger/homeodomain proteins and functions as a DNA-binding transcriptional repressor that interacts with activated SMADs, the transducers of TGF-beta signaling, and interacts with the nucleosome remodeling and histone deacetylation (NURD) complex. By radiation hybrid analysis, this gene is mapped to 2q22. It has been found that synthesis of ZEB2 was upregulated following SNAI1 expression in human cell lines, and the expression of SNAI1 in epithelial cells can trigger an epithelial-mesenchyme transition.
Sequence Similarities: Belongs to the delta-EF1/ZFH-1 C2H2-type zinc-finger family.
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分子量
- 200 kDa
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基因ID
- 9839
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UniProt
- O60315
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途径
- Tube Formation
抗原
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