An amino acid sequence from the N-terminus of human PKM2 (SHGTHEYHAETIKNV) was used as the immunogen for this PKM2 antibody (100% homologous in human, mouse and rat).
The stated application concentrations are suggested starting amounts. Titration of the PKM2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL
限制
仅限研究用
缓冲液
0.5 mg/mL if reconstituted with 0.2 mL sterile DI water
储存条件
-20 °C
储存方法
After reconstitution, the PKM2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Pyruvate Kinase, Muscle, also known as PK3 or PKM2, is an enzyme that in humans is encoded by the PKM gene. The activity of pyruvate kinase subtype M2 is increased by fructose 1, 6-bisphosphate(Fru-1, 6-P2). By in situ hybridization, Popescu and Cheng (1990) mapped the gene to 15q24-q25. Ashizawa et al.(1991) manipulated the intracellular Fru-1, 6-P2 concentration in several mammalian cell lines, including human, by varying the glucose concentration in the media. Using a novel proteomic screen for phosphotyrosine-binding proteins, Christofk et al.(2008) observed that PKM2 binds directly and selectively to tyrosine-phosphorylated peptides.