Paxillin 抗体 (AA 456-472)
Quick Overview for Paxillin 抗体 (AA 456-472) (ABIN3032168)
抗原
See all Paxillin (PXN) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 456-472
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纯化方法
- Antigen affinity
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免疫原
- Amino acids 456-472 (HEKDGKAYCRKDYFDMF) were used as the immunogen for this Paxillin antibody (100% homologous in human, mouse and rat).
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亚型
- IgG
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anti-Paxillin (PXN) (AA 201-461) antibody
PXN 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Paxillin (PXN) (AA 347-578) antibodyPXN 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Paxillin (PXN) (AA 1-262) antibodyPXN 适用: 人 WB, PLA 宿主: 兔 Polyclonal unconjugated
anti-Paxillin (PXN) (AA 327-568) antibodyPXN 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Paxillin (PXN) (AA 456-472), (C-Term) antibodyPXN 适用: 人, 小鼠, 大鼠 WB, IHC, IF, ICC, FACS, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Paxillin (PXN) (pTyr118) antibodyPXN 适用: 人, 小鼠, 大鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Paxillin (PXN) (AA 1-262) antibodyPXN 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-Paxillin (PXN) (pSer178) antibodyPXN 适用: 人 WB, ELISA, IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Paxillin (PXN) (AA 28-45) antibodyPXN 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Paxillin (PXN) (pTyr118) antibodyPXN 适用: 人, 小鼠, 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
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应用备注
- The stated application concentrations are suggested starting amounts. Titration of the Paxillin antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL,IHC (Paraffin): 0.5-1 μg/mL,IHC (Frozen): 0.5-1 μg/mL,Immunocytochemistry: 0.5-1 μg/mL
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限制
- 仅限研究用
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缓冲液
- 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water
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储存条件
- -20 °C
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储存方法
- After reconstitution, the Paxillin antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
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- Paxillin (PXN)
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别名
- Paxillin
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背景
- Paxillin is a signal transduction adaptor protein discovered in 1990 in the laboratory of Keith Burridge. Salgia et al.(1995) mapped the gene to 12q24 using fluorescence in situ hybridization.The C-terminal region of Paxillin contains four LIM domains that target paxillin to focal adhesions, it is presumed through a direct association with the cytoplasmic tail of beta-integrin. The N-terminal region is rich in protein-protein interaction sites. The proteins that bind to Paxillin are diverse and include protein tyrosine kinases, such as Src and FAK, structural proteins, such as vinculin and actopaxin, and regulators of actin organization, such as COOL/PIX and PKL/GIT. Paxillin is tyrosine-phosphorylated by FAK and Src upon integrin engagement or growth factor stimulation, creating binding sites for the adapter protein Crk. The protein contains 4 LIM domains, a proline-rich domain containing a consensus SH3-binding site, and 3 potential SH2-binding sites.
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UniProt
- P49023
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途径
- MAPK Pathway, EGFR Signaling Pathway, Response to Growth Hormone Stimulus, Cell-Cell Junction Organization, Maintenance of Protein Location, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGF Signaling
抗原
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