CDC25B 抗体 (AA 541-559)
Quick Overview for CDC25B 抗体 (AA 541-559) (ABIN3030454)
抗原
See all CDC25B 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 541-559
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纯化方法
- Antigen affinity
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免疫原
- Amino acids 541-559 (DYRPMNHEAFKDELKTFRL-human) were used as the immunogen for this Cdc25B antibody.
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亚型
- IgG
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anti-Cell Division Cycle 25 Homolog B (S. Pombe) (CDC25B) (pSer186) antibody
CDC25B 适用: 小鼠 WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Cell Division Cycle 25 Homolog B (S. Pombe) (CDC25B) (pSer353) antibodyCDC25B 适用: 人, 小鼠 WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Cell Division Cycle 25 Homolog B (S. Pombe) (CDC25B) (pSer323) antibodyCDC25B 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Cell Division Cycle 25 Homolog B (S. Pombe) (CDC25B) (pSer149) antibodyCDC25B 适用: 人, 小鼠 WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Cell Division Cycle 25 Homolog B (S. Pombe) (CDC25B) (pSer323) antibodyCDC25B 适用: 小鼠 WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Cell Division Cycle 25 Homolog B (S. Pombe) (CDC25B) (AA 1-195) antibodyCDC25B 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Cell Division Cycle 25 Homolog B (S. Pombe) (CDC25B) (pSer353) antibodyCDC25B 适用: 人, 小鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Cell Division Cycle 25 Homolog B (S. Pombe) (CDC25B) (AA 331-360) antibodyCDC25B 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal RB07936 unconjugated
anti-Cell Division Cycle 25 Homolog B (S. Pombe) (CDC25B) (AA 1-580) antibodyCDC25B 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated
anti-Cell Division Cycle 25 Homolog B (S. Pombe) (CDC25B) (AA 56-105) antibodyCDC25B 适用: 人, 小鼠, 大鼠, 犬, 豚鼠, 马, Cow, Pig WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
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应用备注
- The stated application concentrations are suggested starting amounts. Titration of the Cdc25B antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL,IHC (Paraffin): 0.5-1 μg/mL
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限制
- 仅限研究用
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缓冲液
- 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water
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储存条件
- -20 °C
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储存方法
- After reconstitution, the Cdc25B antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
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- CDC25B (Cell Division Cycle 25 Homolog B (S. Pombe) (CDC25B))
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别名
- Cdc25B
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背景
- Central to the onset of mitosis in all eukaryotic cells is the CDC2 protein kinase, the activity of which is negatively regulated by phosphorylation and positively activated by dephosphorylation. The latter function is carried out by a specific phosphatase, CDC25. At least 3 human< a href=../search_result.php?search_txt=cdc25>CDC25genes code for the A, B, and C forms of CDC25.< a href=../search_result.php?search_txt=cdc25b>CDC25Bis mapped to 20p13. P38 kinase has a critical role in the initiation of a G2 delay after ultraviolet radiation. Inhibition of p38 blocks the rapid initiation of this checkpoint in both human and murine cells after ultraviolet radiation. In vitro, p38 binds and phosphorylates CDC25B at serines 309 and 361, and< a href=../search_result.php?search_txt=cdc25c>CDC25Cat serine-216, phosphorylation of these residues is required for binding to 14-3-3 proteins. In vivo, inhibition of p38 prevents both phosphorylation of CDC25B at serine-309 and 14-3-3 binding after ultraviolet radiation, and mutation of this site is sufficient to inhibit the checkpoint initiation. Regulation of CDC25B phosphorylation by p38 is a critical event for initiating the G2/M checkpoint after ultraviolet radiation.
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UniProt
- P30305
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途径
- Cell Division Cycle, M Phase, Autophagy
抗原
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