BUB3 抗体 (Middle Region)
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Quick Overview for BUB3 抗体 (Middle Region) (ABIN3030213)
抗原
See all BUB3 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- Middle Region
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纯化方法
- Antigen affinity
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免疫原
- An amino acid sequence from the middle region of human Bub3 (DHQLKMHDLNTDQEN) was used as the immunogen for this Bub3 antibody (100% homologous in human, mouse and rat).
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亚型
- IgG
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anti-Budding Uninhibited By Benzimidazoles 3 Homolog (Yeast) (BUB3) (AA 267-327) antibody
BUB3 适用: 人 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Budding Uninhibited By Benzimidazoles 3 Homolog (Yeast) (BUB3) (AA 76-90), (Middle Region) antibodyBUB3 适用: 人, 小鼠, 大鼠 WB, IHC, ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Budding Uninhibited By Benzimidazoles 3 Homolog (Yeast) (BUB3) (AA 6-217) antibodyBUB3 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Budding Uninhibited By Benzimidazoles 3 Homolog (Yeast) (BUB3) antibodyKD Validated BUB3 适用: 人 WB, FACS 宿主: 兔 Monoclonal 25GB3085 unconjugated Recombinant Antibody
anti-Budding Uninhibited By Benzimidazoles 3 Homolog (Yeast) (BUB3) (N-Term) antibodyBUB3 适用: 人, 小鼠, 大鼠, Cow, 豚鼠, 马, 兔 WB, IF, IHC 宿主: 兔 Polyclonal unconjugated
anti-Budding Uninhibited By Benzimidazoles 3 Homolog (Yeast) (BUB3) (C-Term) antibodyBUB3 适用: 人 WB, ELISA, IF, IHC 宿主: 兔 Polyclonal unconjugated
anti-Budding Uninhibited By Benzimidazoles 3 Homolog (Yeast) (BUB3) (AA 1-328) antibodyBUB3 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Budding Uninhibited By Benzimidazoles 3 Homolog (Yeast) (BUB3) (AA 201-230) antibodyBUB3 适用: Drosophila melanogaster WB 宿主: 兔 Polyclonal RB36332 unconjugated
anti-Budding Uninhibited By Benzimidazoles 3 Homolog (Yeast) (BUB3) (C-Term) antibodyBUB3 适用: 人, 小鼠, 大鼠 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Budding Uninhibited By Benzimidazoles 3 Homolog (Yeast) (BUB3) (C-Term) antibodyBUB3 适用: 人, 小鼠, 大鼠 WB, ELISA, IF, IHC 宿主: 兔 Polyclonal unconjugated
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应用备注
- The stated application concentrations are suggested starting amounts. Titration of the Bub3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL,IHC (Paraffin): 0.5-1 μg/mL,IHC (Frozen): 0.5-1 μg/mL,Immunocytochemistry: 0.5-1 μg/mL
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限制
- 仅限研究用
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缓冲液
- 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water
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储存条件
- -20 °C
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储存方法
- After reconstitution, the Bub3 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
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- BUB3 (Budding Uninhibited By Benzimidazoles 3 Homolog (Yeast) (BUB3))
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别名
- Bub3
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背景
- Budding uninhibited by benzimidazoles 3 is a protein that in humans is encoded by the BUB3 gene. It is a protein involved with the regulation of the Spindle Assembly Checkpoint (SAC), though Bub3 is non-essential in yeast, it is essential in higher eukaryotes. As one of the checkpoint proteins, it delays the irreversible onset of anaphase through direction of kinetochore localization during prometaphase to achieve biorentation. Bub3 directs the localization of Bub1 at the kinetochore in order to activate the SAC. It also acts as a regulator in that it affects binding of Mad3 to Mad2.
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UniProt
- O43684
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途径
- Maintenance of Protein Location
抗原
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