An amino acid sequence from the middle region of human Bub3 (DHQLKMHDLNTDQEN) was used as the immunogen for this Bub3 antibody (100% homologous in human, mouse and rat).
BUB3
适用: 大鼠
WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro)
宿主: 兔
Polyclonal
unconjugated
应用备注
The stated application concentrations are suggested starting amounts. Titration of the Bub3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL,IHC (Paraffin): 0.5-1 μg/mL,IHC (Frozen): 0.5-1 μg/mL,Immunocytochemistry: 0.5-1 μg/mL
限制
仅限研究用
缓冲液
0.5 mg/mL if reconstituted with 0.2 mL sterile DI water
储存条件
-20 °C
储存方法
After reconstitution, the Bub3 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
抗原
BUB3
(Budding Uninhibited By Benzimidazoles 3 Homolog (Yeast) (BUB3))
Budding uninhibited by benzimidazoles 3 is a protein that in humans is encoded by the BUB3 gene. It is a protein involved with the regulation of the Spindle Assembly Checkpoint (SAC), though Bub3 is non-essential in yeast, it is essential in higher eukaryotes. As one of the checkpoint proteins, it delays the irreversible onset of anaphase through direction of kinetochore localization during prometaphase to achieve biorentation. Bub3 directs the localization of Bub1 at the kinetochore in order to activate the SAC. It also acts as a regulator in that it affects binding of Mad3 to Mad2.