12-Lipoxygenase 抗体 (C-Term)
This anti-12-Lipoxygenase antibody is a 兔 多克隆 antibody detecting 12-Lipoxygenase in WB. Suitable for 人.
Quick Overview for 12-Lipoxygenase 抗体 (C-Term) (ABIN3029819)
抗原
12-Lipoxygenase (LOX12)
适用
人
宿主
兔
克隆类型
多克隆
标记
This 12-Lipoxygenase antibody is un-conjugated
应用范围
Western Blotting (WB)
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抗原表位
- C-Term
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纯化方法
- Antigen affinity
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免疫原
- An amino acid sequence from the C-terminus of human ALOX12 (HHKEKYFSGPKPKAVLNQFR) was used as the immunogen for this 12 Lipoxygenase antibody.
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亚型
- IgG
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anti-12-Lipoxygenase (LOX12) (AA 186-231) antibody
LOX12 适用: 人, 小鼠, 大鼠 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
- The stated application concentrations are suggested starting amounts. Titration of the 12 Lipoxygenase antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL
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限制
- 仅限研究用
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缓冲液
- 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water
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储存条件
- -20 °C
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储存方法
- After reconstitution, the 12 Lipoxygenase antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
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- 12-Lipoxygenase (LOX12)
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别名
- 12 Lipoxygenase
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背景
- Arachidonate 12-lipoxygenase, is an enzyme that in humans is encoded by the ALOX12 gene. By fluorescence in situ hybridization, the ALOX12 gene is located in band 17p13.1. The gene consists of 14 exons with 13 introns and spans approximately 15 kb of DNAArachidonate 12-lipoxygenase introduces a molecular oxygen into the C-12 position of arachidonic acid to produce 12(S)-hydroperoxy-5,8,10,14-eicosatetraenoic acid. The major pathway of arachidonic acid metabolism in human platelets proceeds via a 12-lipoxygenase enzyme. Expression of the LOG12 gene was detected in human erythroleukemia cells, platelets, and human umbilical vein endothelial cells by reverse transcription-PCR analysis.
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UniProt
- P18054
抗原
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