Vimentin 抗体
Quick Overview for Vimentin 抗体 (ABIN3026904)
抗原
See all Vimentin (VIM) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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无交叉反应
- 小鼠, 大鼠
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产品特性
- This mAb reacts with a 58 kDa protein identified as vimentin. It shows no cross-reaction with other closely related intermediate filament proteins (IFPs) such as desmin, keratin, neurofilament, and glial fibrillary acid protein. Anti-vimentin alone is of limited value as a diagnostic tool, however, when used in panels with other antibodies, it is useful for the sub-classification of a given tumor. Expression of vimentin, when used in conjunction with anti-keratin, is helpful when distinguishing melanomas from undifferentiated carcinomas and large cell lymphomas. All melanomas and Schwannomas react strongly with anti-vimentin. It labels a variety of mesenchymal cells, including melanocytes, lymphocytes, endothelial cells, and fibroblasts. Non-reactivity of anti-vimentin is often considered more useful than its positive reactivity, since there are a few tumors that do not contain vimentin, e.g. hepatoma and seminoma. Anti-vimentin is also useful as a tissue process control reagent.
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纯化方法
- Protein G affinity chromatography
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免疫原
- Recombinant full-length human protein was used as the immunogen for the anti-Vimentin antibody.
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亚型
- IgG1
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anti-Vimentin (VIM) (AA 371-466) antibody
VIM 适用: 人, 小鼠, 大鼠, Pig, Cow, 猴 WB, ELISA, FACS, IHC (p), ICC, IHC (fro), IF (p), IF (cc) 宿主: 兔 Polyclonal unconjugated
anti-Vimentin (VIM) antibodyKO Validated VIM 适用: 人 WB, IHC, ELISA, IF, IHC (p), ICC, IP, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Vimentin (VIM) (AA 435-466), (C-Term) antibodyVIM 适用: 人, 小鼠, 大鼠 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Vimentin (VIM) antibodyVIM 适用: 人, 小鼠, 大鼠, Pig, 小鸡 WB, IHC (p), ICC, IP 宿主: 小鼠 Monoclonal VI-10 unconjugated
anti-Vimentin (VIM) antibodyVIM 适用: 哺乳动物 WB, FACS, IHC (p), ICC 宿主: 小鼠 Monoclonal VI-01 unconjugated
anti-Vimentin (VIM) (AA 1-200) antibodyVIM 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Vimentin (VIM) antibodyVIM 适用: 人 WB, IHC, IF 宿主: 小鼠 Monoclonal C12 unconjugated
anti-Vimentin (VIM) (AA 41-140) antibodyVIM 适用: 人 WB, ELISA, IF, IP 宿主: 小鼠 Monoclonal 3E9 unconjugated
anti-Vimentin (VIM) antibodyVIM 适用: 人, Pig, Cow, 小鸡, 犬, Cat, 山羊 WB, IHC, FACS, StM 宿主: 小鼠 Monoclonal VM452 unconjugated
anti-Vimentin (VIM) antibodyVIM 适用: 人 WB, IHC, StM 宿主: 兔 Monoclonal VIM-1937R unconjugated Recombinant Antibody
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应用备注
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Optimal dilution of the anti-Vimentin antibody should be determined by the researcher.
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 1-2 μg/mL,Western blot: 0.25-0.5 μg/mL,Immunohistochemistry (FFPE): 0.25-0.5 μg/mL for 30 min at RT (1),Prediluted format: incubate for 30 min at RT (2) -
限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- 1 mg/mL in 1X PBS, BSA free, sodium azide free
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储存液
- Azide free
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储存条件
- 4 °C,-20 °C
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储存方法
- Store the anti-Vimentin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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- Vimentin (VIM)
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别名
- Vimentin
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背景
- This mAb reacts with a 58 kDa protein identified as vimentin. It shows no cross-reaction with other closely related intermediate filament proteins (IFPs) such as desmin, keratin, neurofilament, and glial fibrillary acid protein. Anti-vimentin alone is of limited value as a diagnostic tool, however, when used in panels with other antibodies, it is useful for the sub-classification of a given tumor. Expression of vimentin, when used in conjunction with anti-keratin, is helpful when distinguishing melanomas from undifferentiated carcinomas and large cell lymphomas. All melanomas and Schwannomas react strongly with anti-vimentin. It labels a variety of mesenchymal cells, including melanocytes, lymphocytes, endothelial cells, and fibroblasts. Non-reactivity of anti-vimentin is often considered more useful than its positive reactivity, since there are a few tumors that do not contain vimentin, e.g. hepatoma and seminoma. Anti-vimentin is also useful as a tissue process control reagent.
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途径
- Caspase Cascade in Apoptosis
抗原
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