NEFH 抗体
Quick Overview for NEFH 抗体 (ABIN3026888)
抗原
See all NEFH 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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产品特性
- This mAb reacts with a 200 kDa protein, identified as heavy sub-unit of neurofilaments (NF-H). It reacts specifically with the phosphorylated KSP/KEP segment at the C-terminus of the heavy subunit (NF-H) of neurofilaments. After dephosphorylation of neurofilaments with alkaline phosphatase, this Ab no longer binds. Neurofilaments make up the main structural elements of axons and dendrites and are found in neurons, peripheral nerves, and sympathetic ganglion cells. Neurofilaments consist of three major subunits with molecular weights of 68 kDa (NF-L), 160 kDa (NF-M) and 200 kDa (NF-H). Anti-neurofilament stains a number of neural, neuroendocrine, and endocrine tumors. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas, and neuroblastomas stain positively for anti-neurofilament. Neurofilaments are also present in paragangliomas as well as adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and cell carcinomas of the lung also express neurofilament.
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纯化方法
- Protein G affinity chromatography
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免疫原
- Crude neurofilament preparation from porcine spinal cord was used as the immunogen for the NF-H antibody (phospho).
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亚型
- IgG1
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anti-Neurofilament, Heavy Polypeptide (NEFH) antibody
NEFH 适用: 人, 大鼠, 小鼠, 小鸡, Pig IHC, FACS, IF, StM 宿主: 小鼠 Monoclonal NF421 unconjugated
anti-Neurofilament, Heavy Polypeptide (NEFH) (AA 2-251) antibodyNEFH 适用: 人 IHC, FACS, ELISA 宿主: 小鼠 Monoclonal 4F2G12 unconjugated
anti-Neurofilament, Heavy Polypeptide (NEFH) (AA 2-251) antibodyNEFH 适用: 人 IHC, ELISA 宿主: 小鼠 Monoclonal 2C12F3 unconjugated
anti-Neurofilament, Heavy Polypeptide (NEFH) (AA 1-100) antibodyNEFH 适用: 人, 大鼠, 小鼠 WB, ELISA, IHC (p), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-Neurofilament, Heavy Polypeptide (NEFH) antibodyNEFH 适用: 人, 大鼠, 小鼠, 小鸡, Pig, Cow, 兔, 豚鼠, Gerbil, Cat WB, IHC, FACS, StM 宿主: 小鼠 Monoclonal NE14 unconjugated
anti-Neurofilament, Heavy Polypeptide (NEFH) antibodyNEFH 适用: Cow WB, IF 宿主: 小鸡 Polyclonal unconjugated
anti-Neurofilament, Heavy Polypeptide (NEFH) (AA 968-1020) antibodyNEFH 适用: 人 IHC, ELISA 宿主: 小鼠 Monoclonal 8H10 unconjugated
anti-Neurofilament, Heavy Polypeptide (NEFH) (AA 968-1020) antibodyNEFH 适用: 人 WB, IHC, ELISA 宿主: 小鼠 Monoclonal unconjugated
anti-Neurofilament, Heavy Polypeptide (NEFH) (AA 21-120) antibodyNEFH 适用: 人, 大鼠, 小鸡 WB, FACS, ELISA, IHC (p), ICC, IHC (fro), IF (p), IF (cc) 宿主: 兔 Polyclonal unconjugated
anti-Neurofilament, Heavy Polypeptide (NEFH) antibodyNEFH 适用: 人, 大鼠, 小鼠, 小鸡, Pig WB, IHC, FACS, StM 宿主: 小鼠 Monoclonal rNF421 unconjugated Recombinant Antibody
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应用备注
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Optimal dilution of the NF-H antibody (phospho) should be determined by the researcher.
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunohistochemistry (FFPE): 0.25-0.5 μg/mL for 30 min at RT (1),Prediluted format: incubate for 30 min at RT (2) -
限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- 1 mg/mL in 1X PBS, BSA free, sodium azide free
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储存液
- Azide free
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储存条件
- 4 °C,-20 °C
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储存方法
- Store the NF-H antibody (phospho) at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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- NEFH (Neurofilament, Heavy Polypeptide (NEFH))
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别名
- NF-H
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背景
- This mAb reacts with a 200 kDa protein, identified as heavy sub-unit of neurofilaments (NF-H). It reacts specifically with the phosphorylated KSP/KEP segment at the C-terminus of the heavy subunit (NF-H) of neurofilaments. After dephosphorylation of neurofilaments with alkaline phosphatase, this Ab no longer binds. Neurofilaments make up the main structural elements of axons and dendrites and are found in neurons, peripheral nerves, and sympathetic ganglion cells. Neurofilaments consist of three major subunits with molecular weights of 68 kDa (NF-L), 160 kDa (NF-M) and 200 kDa (NF-H). Anti-neurofilament stains a number of neural, neuroendocrine, and endocrine tumors. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas, and neuroblastomas stain positively for anti-neurofilament. Neurofilaments are also present in paragangliomas as well as adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and cell carcinomas of the lung also express neurofilament.
抗原
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