Multi Cytokeratin 4/5/6/8/10/13/18 抗体
The 小鼠 单克隆 anti-Multi Cytokeratin 4/5/6/8/10/13/18 antibody is suitable to detect Multi Cytokeratin 4/5/6/8/10/13/18 in samples from Cow, Frog, 山羊, 豚鼠, 人, Marmoset, 小鼠, Pig 和 大鼠. It has been validated for FACS, IF, IHC (p) 和 WB.
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产品编号 ABIN3026733
发货至:
中国
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Contact Info
Our Local Distributor
中国
北京 101111
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
Quick Overview for Multi Cytokeratin 4/5/6/8/10/13/18 抗体 (ABIN3026733)
抗原
Multi Cytokeratin 4/5/6/8/10/13/18
适用
Cow, Frog, 山羊, 豚鼠, 人, Marmoset, 小鼠, Pig, 大鼠
宿主
小鼠
克隆类型
单克隆
标记
非结合性
应用范围
Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
克隆位点
C11
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产品特性
- This antibody recognizes multi cytokeratins: 4, 5, 6, 8, 10, 13, and 18. It is a broad-spectrum cytokeratin antibody which has been reported to be used to differentiate epithelial tumors from non-epithelial tumors. Many studies have shown the usefulness of cytokeratin markers in cancer research and tumor diagnosis.
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纯化方法
- Protein G affinity chromatography
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免疫原
- A keratin-enriched preparation from cultured human A431 was used as the immunogen for this Multi Cytokeratin antibody.
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亚型
- IgG1
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应用备注
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The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Multi Cytokeratin antibody to be titered up or down for optimal performance.
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. FACS: use the Multi Cytokeratin mAb at 0.5-1 μg/million cells,IF: 0.5-1 μg/mL,WB: 0.5-1 μg/mL,IHC (FFPE): 0.5-1 μg/mL for 30 min at RT (1),Prediluted format : incubate for 30 min at RT (2) -
限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- 1 mg/mL in 1X PBS, BSA free, sodium azide free
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储存液
- Azide free
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储存条件
- 4 °C,-20 °C
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储存方法
- Store the Multi Cytokeratin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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- Multi Cytokeratin 4/5/6/8/10/13/18
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背景
- This antibody recognizes multi cytokeratins: 4, 5, 6, 8, 10, 13, and 18. It is a broad-spectrum cytokeratin antibody which has been reported to be used to differentiate epithelial tumors from non-epithelial tumors. Many studies have shown the usefulness of cytokeratin markers in cancer research and tumor diagnosis.
抗原
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