Multi Cytokeratin 4/5/6/8/10/13/18 抗体
-
- 抗原
- Multi Cytokeratin 4/5/6/8/10/13/18
- 适用
- Marmoset, 小鼠, Pig, Cow, 大鼠, Frog, 山羊, 豚鼠, 人
-
宿主
- 小鼠
-
克隆类型
- 单克隆
-
标记
- 非结合性
- 应用范围
- Flow Cytometry (FACS), Immunofluorescence (IF), Western Blotting (WB), Immunohistochemistry (IHC)
- 纯化方法
- Protein G affinity chromatography
- 免疫原
- A keratin-enriched preparation from cultured human A431 was used as the immunogen for this Multi Cytokeratin antibody.
- 克隆位点
- C11
- 亚型
- IgG1
-
anti-Multi Cytokeratin 4/5/6/8/10/13/18 antibody
适用: Marmoset, 小鼠, Pig, Cow, 大鼠, Frog, 山羊, 豚鼠, 人 FACS, IF, IHC (p), WB 宿主: 小鼠 Monoclonal C11 unconjugated
-
- 应用备注
-
Flow Cytometry: use the Multi Cytokeratin mAb at 0.5-1 μg/million cells
IF: 0.5-1 μg/mL
WB: 0.5-1 μg/mL
IHC (FFPE): 0.5-1 μg/mL for 30 min at RT (1)
Prediluted format : incubate for 30 min at RT (2)
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Multi Cytokeratin antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. - 限制
- 仅限研究用
-
- 状态
- Liquid
- 浓度
- 0.2 mg/mL
- 缓冲液
- PBS with 0.1 mg/mL BSA and 0.05 % sodium azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- -20 °C
- 储存方法
- Aliquot and Store at -20C. Avoid freez-thaw cycles.
-
- 抗原
- Multi Cytokeratin 4/5/6/8/10/13/18
- 背景
- This antibody recognizes multi cytokeratins: 4, 5, 6, 8, 10, 13, and 18. It is a broad-spectrum cytokeratin antibody which has been reported to be used to differentiate epithelial tumors from non-epithelial tumors. Many studies have shown the usefulness of cytokeratin markers in cancer research and tumor diagnosis.
- 基因ID
- 3851, 3852, 3853
-
