Multi Cytokeratin 4/5/6/8/10/13/18 抗体
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北京 101111
Quick Overview for Multi Cytokeratin 4/5/6/8/10/13/18 抗体 (ABIN6252020)
抗原
适用
宿主
克隆类型
标记
应用范围
克隆位点
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纯化方法
- Protein G affinity chromatography
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免疫原
- A keratin-enriched preparation from cultured human A431 was used as the immunogen for this Multi Cytokeratin antibody.
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亚型
- IgG1
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anti-Multi Cytokeratin 4/5/6/8/10/13/18 antibody
适用: Cow, Frog, 山羊, 豚鼠, 人, Marmoset, 小鼠, Pig, 大鼠 FACS, IF, IHC (p), WB 宿主: 小鼠 Monoclonal C11 unconjugated
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应用备注
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Flow Cytometry: use the Multi Cytokeratin mAb at 0.5-1 μg/million cells
IF: 0.5-1 μg/mL
WB: 0.5-1 μg/mL
IHC (FFPE): 0.5-1 μg/mL for 30 min at RT (1)
Prediluted format : incubate for 30 min at RT (2)
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Multi Cytokeratin antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.2 mg/mL
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缓冲液
- PBS with 0.1 mg/mL BSA and 0.05 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Aliquot and Store at -20C. Avoid freez-thaw cycles.
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- Multi Cytokeratin 4/5/6/8/10/13/18
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背景
- This antibody recognizes multi cytokeratins: 4, 5, 6, 8, 10, 13, and 18. It is a broad-spectrum cytokeratin antibody which has been reported to be used to differentiate epithelial tumors from non-epithelial tumors. Many studies have shown the usefulness of cytokeratin markers in cancer research and tumor diagnosis.
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基因ID
- 3851, 3852, 3853
抗原
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