Involucrin 抗体
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北京 101111
Quick Overview for Involucrin 抗体 (ABIN3026654)
抗原
See all Involucrin (IVL) 抗体适用
宿主
克隆类型
标记
应用范围
质量等级
克隆位点
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原理
- Involucrin Antibody (azide and preservative free)
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无交叉反应
- 小鼠
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产品特性
- This antibody recognizes a protein of 66-170 kDa, identified as involucrin. In Western blotting of cultured human keratinocytes, this antibody reacts with a 120 kDa protein. It stains the involucrin in a variety of sizes: 170 kDa in MCF-7 cells, a doublet of ~115 and 150 kDa in gorilla and owl monkey, 66 kDa in dog, and a doublet of 105 kDa in pig. Its epitope maps between amino acids 421-568 of human involucrin. Involucrin is expressed in a range of stratified squamous epithelia, including the cornea, which lacks a distinct cornified layer. In normal epidermis, it is first expressed in the upper spinous layers, and in keratinocyte cultures, all cells that have left the basal layer express it. Involucrin expression is altered in pathological conditions: in psoriasis and other benign epidermal hyperplasias, involucrin expression begins closer to the basal layer than normal, expression is abnormal in squamous cell carcinomas and premalignant lesions, and is reduced in severe dysplasias of the larynx and cervix.
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纯化方法
- Protein G affinity chromatography
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免疫原
- Purified Involucrin from human keratinocytes was used as the immunogen for this antibody.
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亚型
- IgG1, kappa
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anti-Involucrin (IVL) (AA 461-551) antibody
IVL 适用: 大鼠 IHC, WB, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Involucrin (IVL) antibodyIVL 适用: 人 IHC, WB 宿主: 兔 Monoclonal unconjugated
anti-Involucrin (IVL) (AA 65-180) antibodyIVL 适用: 人 ELISA, IHC (p), ICC, IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Involucrin (IVL) (AA 301-460) antibodyIVL 适用: 小鼠 IHC, WB, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Involucrin (IVL) antibodyIVL 适用: 人, 小鼠 IHC, WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Involucrin (IVL) (AA 24-118) antibodyIVL 适用: 人 IHC, WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Involucrin (IVL) antibodyIVL 适用: 人, 小鼠 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Involucrin (IVL) (AA 36-152) antibodyIVL 适用: 人 IHC, WB, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Involucrin (IVL) antibodyIVL 适用: 人, 犬, Pig, Gorilla, Owl Monkey IHC, WB 宿主: 小鼠 Monoclonal rIVRN-827 unconjugated Recombinant Antibody
anti-Involucrin (IVL) antibodyIVL 适用: 人, 犬, Pig, Gorilla, Owl Monkey IHC 宿主: 小鼠 Monoclonal SY5 unconjugated
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应用备注
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The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.
1. Staining of formalin-fixed tissues is enhanced by digestion with trypsin Protease XXV (Sigma) at 1 mg/mL PBS for 5 min at 37 °C.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- 1 mg/mL in 1X PBS, BSA free, sodium azide free
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储存液
- Azide free
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储存条件
- 4 °C,-20 °C
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储存方法
- Store the Involucrin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
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- Involucrin (IVL)
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别名
- Involucrin
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背景
- This antibody recognizes a protein of 66-170 kDa, identified as involucrin. In Western blotting of cultured human keratinocytes, this antibody reacts with a 120 kDa protein. It stains the involucrin in a variety of sizes: 170 kDa in MCF-7 cells, a doublet of ~115 and 150 kDa in gorilla and owl monkey, 66 kDa in dog, and a doublet of 105 kDa in pig. Its epitope maps between amino acids 421-568 of human involucrin. Involucrin is expressed in a range of stratified squamous epithelia, including the cornea, which lacks a distinct cornified layer. In normal epidermis, it is first expressed in the upper spinous layers, and in keratinocyte cultures, all cells that have left the basal layer express it. Involucrin expression is altered in pathological conditions: in psoriasis and other benign epidermal hyperplasias, involucrin expression begins closer to the basal layer than normal, expression is abnormal in squamous cell carcinomas and premalignant lesions, and is reduced in severe dysplasias of the larynx and cervix.
抗原
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