Cyclin A 抗体
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- 抗原 See all Cyclin A (CCNA2) 抗体
- Cyclin A (CCNA2) (Cyclin A2 (CCNA2))
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适用
- 人, 小鼠, Cow, Green Monkey, Mink
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This Cyclin A antibody is un-conjugated
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应用范围
- Immunofluorescence (IF), Flow Cytometry (FACS)
- 产品特性
- This antibody recognizes a protein of 50-55 kDa, which is identified as Cyclin A. Its epitope is located amino acids 144-148 of the human protein. Cyclins are regulatory subunits of the cyclin-dependent kinases (cdk's) and they control transition at different specific phases of the cell cycle. The temporal expression of cyclins is tightly regulated and subsequently plays a critical role in controlling the enzymatic activity of cdk's. These cyclin/cdk complexes are essential for passage through specific stages in the cell cycle. In mammalian somatic cells, Cyclin A is required for S-phase and passage through G2-phase. The D and E type cyclins regulate the passage of G1, while Cyclin B is a critical regulator of mitosis. Mutation or disruption of normal Cyclin A expression causes cells to arrest in G2-phase.
- 纯化方法
- Protein G affinity chromatography
- 免疫原
- Full length bovine protein was used as the immunogen for this CCNA2 antibody.
- 克隆位点
- E67
- 亚型
- IgG2a kappa
- Top Product
- Discover our top product CCNA2 Primary Antibody
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- 应用备注
- The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Cyclin A2 antibody to be titered up or down for optimal performance.\. FACS: 0.5-1 μg/million cells,IF: 1-2 μg/mL,
- 限制
- 仅限研究用
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- 浓度
- 1 mg/mL
- 缓冲液
- 1 mg/mL in 1X PBS, BSA free, sodium azide free
- 储存液
- Azide free
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store the Cyclin A2 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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- 抗原
- Cyclin A (CCNA2) (Cyclin A2 (CCNA2))
- 别名
- Cyclin A2 (CCNA2 产品)
- 别名
- CCN1 antibody, CCNA antibody, AA408589 antibody, Ccn-1 antibody, Ccn1 antibody, Ccna antibody, CycA2 antibody, Cyca antibody, cb671 antibody, chunp6924 antibody, wu:fi36f03 antibody, CYCA antibody, Cyclin-A2 antibody, ccna2 antibody, ccn1 antibody, ccna antibody, ccna1 antibody, Cyclin-A antibody, cyclinA antibody, cyclin A2 antibody, cyclin A2 S homeolog antibody, cyclin A2 L homeolog antibody, CCNA2 antibody, Ccna2 antibody, ccna2 antibody, ccna2.S antibody, ccna2.L antibody
- 背景
- This antibody recognizes a protein of 50-55 kDa, which is identified as Cyclin A. Its epitope is located amino acids 144-148 of the human protein. Cyclins are regulatory subunits of the cyclin-dependent kinases (cdk's) and they control transition at different specific phases of the cell cycle. The temporal expression of cyclins is tightly regulated and subsequently plays a critical role in controlling the enzymatic activity of cdk's. These cyclin/cdk complexes are essential for passage through specific stages in the cell cycle. In mammalian somatic cells, Cyclin A is required for S-phase and passage through G2-phase. The D and E type cyclins regulate the passage of G1, while Cyclin B is a critical regulator of mitosis. Mutation or disruption of normal Cyclin A expression causes cells to arrest in G2-phase.
- 基因ID
- 890
- 途径
- PI3K-Akt Signaling, Cell Division Cycle, AMPK Signaling, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Synthesis of DNA
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