CD20 抗体
Quick Overview for CD20 抗体 (ABIN3026620)
抗原
See all CD20 (MS4A1) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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无交叉反应
- 牛, 犬, 猪, 大鼠
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产品特性
- This antibody recognizes a protein of 33-37 kDa, identified as CD20 (Workshop V, Code CD20.12). The antibody recognizes the extracellular domain of the protein. The epitope is similar to or identical to that recognized by other CD20 antibodies including Leu-16 and B1. This antibody can be used for immunophenotyping of leukemia and malignant cells, B lymphocyte detection in peripheral blood, Bcell localization in tissues and B lymphocyte purification by immunosorbent methods. CD20 is a non-Ig differentiation antigen of Bcells and its expression is restricted to normal and neoplastic Bcells, being absent from all other leukocytes and tissues. It is expressed by pre Bcells and persists during all stages of Bcell maturation but is lost upon terminal differentiation into plasma cells. Protein passes through the membrane 4 times with both ends in cytoplasm and exposes one short and one longer loop to the external environment. CD20 is not glycosylated in resting Bcells and its cytoplasmic domains are differentially phosphorylated upon activation. It acts as a calcium channel involved in Bcell activation and cell cycle progression.
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免疫原
- Human tonsil B cells (L26) and recombinant protein (IGEL/733) were used as the immunogen for this CD20 antibody cocktail.
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亚型
- IgG2a kappa
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anti-Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1) (C-Term) antibody
Verified MS4A1 适用: 人 WB, IHC, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1) antibodyMS4A1 适用: 人 FACS, WB, IHC, IF, StM 宿主: 小鼠 Monoclonal L26 unconjugated
anti-Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1) (AA 213-297) antibodyMS4A1 适用: 人 FACS, WB, IHC, IF, ELISA, StM, Coat 宿主: 小鼠 Monoclonal MS4A1-3409 unconjugated
anti-Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1) antibodyMS4A1 适用: 人 WB, IHC, IF 宿主: 兔 Monoclonal unconjugated
anti-Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1) (AA 1-297) antibodyMS4A1 适用: 人 FACS, WB, IHC (p) 宿主: 小鼠 Polyclonal unconjugated
anti-Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1) antibodyMS4A1 适用: 人 FACS, IHC, IF, StM 宿主: 小鼠 Monoclonal rIGEL-773 unconjugated Recombinant Antibody
anti-Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1) antibodyMS4A1 适用: 人, Cow FACS, IP 宿主: 小鼠 Monoclonal MEM-97 unconjugated
anti-Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1) antibodyMS4A1 适用: 人, Cow FACS, IP 宿主: 小鼠 Monoclonal MEM-97 unconjugated
anti-Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1) (AA 1-297) antibodyMS4A1 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1) (AA 210-297) antibodyMS4A1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 5C11 unconjugated
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应用备注
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The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the CD20 antibody cocktail to be titered up or down for optimal performance.
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.\. Flow Cytometry: 5-10 μL/million cells,Immunofluorescence: 1:50-1:100,Immunohistology (FFPE): 1:100-1:200 for 30 minutes at RT (1)
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- Bioreactor concentrate with 0.05 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C,4 °C
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储存方法
- CD20 antibody c°Cktail with azide can be stored at 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.
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- CD20 (MS4A1) (Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1))
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别名
- CD20
抗原
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