KIT 抗体
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- 抗原 See all KIT 抗体
- KIT (Mast/stem Cell Growth Factor Receptor (KIT))
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This KIT antibody is un-conjugated
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应用范围
- Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 产品特性
- This antibody recognizes a protein of 145 kDa, identified as CD117, or p145kit. It is found on a wide variety of tumor cells including follicular and papillary carcinoma of thyroid, adenocarcinomas from endometrium, lung, ovary, pancreas, and breast as well as malignant melanoma, endodermal sinus tumor, and small cell carcinoma. However, CD117 antibody has been particularly useful in differentiating gastrointestinal stromal tumors from Kaposi's sarcoma, tumors of smooth muscle origin, fibromatosis, and neural tumors of the GI tract. CD117 antibody is also useful in recognizing myeloblasts in bone marrow biopsy and clot section.
- 纯化方法
- Protein G affinity chromatography
- 免疫原
- Recombinant human protein was used as the immunogen for this CD117 antibody.
- 克隆位点
- C117-370
- 亚型
- IgG1 kappa
- Top Product
- Discover our top product KIT Primary Antibody
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- 应用备注
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The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the CD117 antibody to be titered up or down for optimal performance.
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.\. IHC (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1),FACS: 0.5-1 μg/million cells in 0.1ml,IF: 0.5-1 μg/mL
- 限制
- 仅限研究用
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- 浓度
- 1 mg/mL
- 缓冲液
- 1 mg/mL in 1X PBS, BSA free, sodium azide free
- 储存液
- Azide free
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store the CD117 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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- 抗原
- KIT (Mast/stem Cell Growth Factor Receptor (KIT))
- 别名
- CD117 (KIT 产品)
- 背景
- This antibody recognizes a protein of 145 kDa, identified as CD117, or p145kit. It is found on a wide variety of tumor cells including follicular and papillary carcinoma of thyroid, adenocarcinomas from endometrium, lung, ovary, pancreas, and breast as well as malignant melanoma, endodermal sinus tumor, and small cell carcinoma. However, CD117 antibody has been particularly useful in differentiating gastrointestinal stromal tumors from Kaposi's sarcoma, tumors of smooth muscle origin, fibromatosis, and neural tumors of the GI tract. CD117 antibody is also useful in recognizing myeloblasts in bone marrow biopsy and clot section.
- 基因ID
- 3815
- 途径
- RTK signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Sensory Perception of Sound, Stem Cell Maintenance, Production of Molecular Mediator of Immune Response, Regulation of long-term Neuronal Synaptic Plasticity
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