MLANA 抗体
Quick Overview for MLANA 抗体 (ABIN3026559)
抗原
See all MLANA 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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产品特性
- This antibody recognizes a protein doublet of 20-22 kDa, identified as MART-1 (Melanoma Antigen Recognized by T cells 1) or Melan-A. MART-1 is a melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. There are seven other melanoma associated antigens recognized by autologous cytotoxic T cells: MAGE-1, MAGE-3, tyrosinase, gp100, gp75, BAGE-1, and GAGE-1. Subcellular fractionation shows that MART-1 is present in melanosomes and endoplasmic reticulum. This MART-1 antibody labels melanomas and other tumors showing melanocytic differentiation. It is also a useful positive-marker for angiomyolipomas. The antibody does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin.
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纯化方法
- Protein G affinity chromatography
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免疫原
- Recombinant human Melan-A protein was used as the immunogen for this antibody.
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亚型
- IgG2b kappa
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anti-Melan A (MLANA) antibody
MLANA 适用: 人, 小鼠, 大鼠 IHC, ISt, StM 宿主: 兔 Monoclonal MLANA-1761R unconjugated Recombinant Antibody
anti-Melan A (MLANA) antibodyMLANA 适用: 人, 小鼠, 大鼠 WB, IF, FACS, IHC, ISt, StM 宿主: 小鼠 Monoclonal M2-9E3 unconjugated
anti-Melan A (MLANA) antibodyMLANA 适用: 人, 马 IHC, ISt, StM 宿主: 小鼠 Monoclonal MLANA-1761R unconjugated
anti-Melan A (MLANA) (AA 48-118) antibodyMLANA 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Melan A (MLANA) (AA 1-118) antibodyMLANA 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Melan A (MLANA) antibodyMLANA 适用: 人, 小鼠, 大鼠, 犬 WB, IF, FACS, IHC, ISt, StM 宿主: 小鼠 Monoclonal A103 unconjugated
anti-Melan A (MLANA) antibodyMLANA 适用: 人, 小鼠, 大鼠, 犬 IHC, ISt, StM 宿主: 兔 Monoclonal MLANA-1409R unconjugated Recombinant Antibody
anti-Melan A (MLANA) antibodyMLANA 适用: 人, 小鼠, 大鼠, 犬 IHC, ISt, StM 宿主: 小鼠 Monoclonal rMLANA-788 unconjugated Recombinant Antibody
anti-Melan A (MLANA) antibodyMLANA 适用: 人 IHC 宿主: 小鼠 Monoclonal MSVA-901M- unconjugated Recombinant Antibody
anti-Melan A (MLANA) (AA 48-118) antibodyMLANA 适用: 人 ELISA 宿主: 小鼠 Monoclonal 2F3B7 unconjugated
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应用备注
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The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Melan-A antibody to be titered up or down for optimal performance.
1. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. ELISA: order BSA-free format for coating,FACS: 0.5-1 μg/million cells,IF: 1-2 μg/mL,WB: 0.5-1 μg/mL,IP: 0.5-1 μg/500 μg protein lysate,IHC (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1),Prediluted format : incubate for 30 min at RT (2) -
限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- 1 mg/mL in 1X PBS, BSA free, sodium azide free
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储存液
- Azide free
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储存条件
- 4 °C,-20 °C
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储存方法
- Store the Melan-A antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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- MLANA (Melan A (MLANA))
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别名
- Melan-A
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背景
- This antibody recognizes a protein doublet of 20-22 kDa, identified as MART-1 (Melanoma Antigen Recognized by T cells 1) or Melan-A. MART-1 is a melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. There are seven other melanoma associated antigens recognized by autologous cytotoxic T cells: MAGE-1, MAGE-3, tyrosinase, gp100, gp75, BAGE-1, and GAGE-1. Subcellular fractionation shows that MART-1 is present in melanosomes and endoplasmic reticulum. This MART-1 antibody labels melanomas and other tumors showing melanocytic differentiation. It is also a useful positive-marker for angiomyolipomas. The antibody does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin.
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基因ID
- 2315
抗原
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