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ODC1 抗体

This anti-ODC1 antibody is a 小鼠 单克隆 antibody detecting ODC1 in WB, IF, FACS 和 IHC (p). Suitable for 人 和 大鼠.
产品编号 ABIN3026477
发货至: 中国

Quick Overview for ODC1 抗体 (ABIN3026477)

抗原

See all ODC1 抗体
ODC1 (Ornithine Decarboxylase 1 (ODC1))

适用

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人, 大鼠

宿主

  • 86
  • 50
  • 2
小鼠

克隆类型

  • 76
  • 62
单克隆

标记

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This ODC1 antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

克隆位点

ODC1-485
  • 产品特性

    This antibody recognizes a 53 kDa protein, identified as the Ornithine Decarboxylase. ODC-1 is the initial and rate-limiting enzyme in the biosynthetic pathway of polyamines and is involved in the conversion of ornithine to putrescine. The biological activity of ODC-1 is rapidly induced in response to virtually all agents known to promote cell proliferation including hormones, drugs, growth factors, mitogens, and tumor promoters. Reportedly, ODC-1 mRNA levels are elevated in lung carcinomas as well as in colon adenomas and carcinomas. Activity in colorectal carcinomas is greater than those in adenomas and normal mucosa.

    纯化方法

    Protein G affinity chromatography

    免疫原

    Recombinant human ODC-1 protein was used as the immunogen for this antibody.

    亚型

    IgG1 kappa
  • 应用备注

    The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.

    1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
    2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. FACS: 0.5-1 μg/million cells,IF: 0.5-1 μg/mL,WB: 0.5-1 μg/mL,IHC (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1),Prediluted format : incubate for 30 min at RT (2)

    限制

    仅限研究用
  • 浓度

    1 mg/mL

    缓冲液

    1 mg/mL in 1X PBS, BSA free, sodium azide free

    储存液

    Azide free

    储存条件

    4 °C,-20 °C

    储存方法

    Store the ODC-1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
  • 抗原

    ODC1 (Ornithine Decarboxylase 1 (ODC1))

    别名

    ODC-1 / Ornithine Decarboxylase 1

    背景

    This antibody recognizes a 53  kDa protein, identified as the Ornithine Decarboxylase. ODC-1 is the initial and rate-limiting enzyme in the biosynthetic pathway of polyamines and is involved in the conversion of ornithine to putrescine. The biological activity of ODC-1 is rapidly induced in response to virtually all agents known to promote cell proliferation including hormones, drugs, growth factors, mitogens, and tumor promoters. Reportedly, ODC-1 mRNA levels are elevated in lung carcinomas as well as in colon adenomas and carcinomas. Activity in colorectal carcinomas is greater than those in adenomas and normal mucosa.

    基因ID

    4953
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