CD45RO 抗体
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北京 101111
Quick Overview for CD45RO 抗体 (ABIN3026460)
抗原
适用
宿主
克隆类型
标记
应用范围
克隆位点
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产品特性
- This antibody recognizes a 180-185 kDa protein, identified as isoform of leukocyte common antigen (CD45) called CD45RO (4th Leucocyte Typing Workshop: Code No. N31). The epitope recognized by this antibody is sensitive to neuraminidase digestion. CD45RO is found on mature activated T-cells, most thymocytes, and a sub-population of resting T-cells within both CD4 and CD8 subsets. The CD45RO antibody shows no reactivity with normal B or natural killer cells, but reacts with granulocytes and monocytes. Reportedly, antibody to CD45RO is useful to identify T-cell lymphomas and leukemias. It rarely stains NK cells or B-cell lymphomas.
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纯化方法
- Protein G affinity chromatography
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免疫原
- Cultured human T-cells from an IL-2-dependent T-cell line (CA1) were used as the immunogen for the CD45RO antibody.
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亚型
- IgG2a kappa
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anti-CD45RO (CD45RO) antibody
CD45RO 适用: 人, 小鼠, 大鼠 WB, IHC (p), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-CD45RO (CD45RO) antibody (PE)CD45RO 适用: Cow, 山羊 FACS 宿主: 小鼠 Monoclonal IL-A116 PE
anti-CD45RO (CD45RO) antibodyGMP Grade CD45RO 适用: 人 FACS, IHC, IP 宿主: 小鼠 Monoclonal UCHL1 unconjugated
anti-CD45RO (CD45RO) antibody (FITC)GMP Grade CD45RO 适用: 人 FACS 宿主: 小鼠 Monoclonal UCHL1 FITC
anti-CD45RO (CD45RO) antibody (mFluor™450)GMP Grade CD45RO 适用: 人 FACS 宿主: 小鼠 Monoclonal UCHL1 mFluor™450
anti-CD45RO (CD45RO) antibody (APC)GMP Grade CD45RO 适用: 人 FACS 宿主: 小鼠 Monoclonal UCHL1 APC
anti-CD45RO (CD45RO) antibody (PE)GMP Grade (Analyte) CD45RO 适用: 人 FACS 宿主: 小鼠 Monoclonal UCHL1 PE
anti-CD45RO (CD45RO) antibody (PE-CF®594)GMP Grade (Analyte) CD45RO 适用: 人 FACS 宿主: 小鼠 Monoclonal UCHL1 PE-CF®594
anti-CD45RO (CD45RO) antibody (FITC)CD45RO 适用: 人 FACS, IHC, IHC (p), IP, IHC (fro) 宿主: 小鼠 Monoclonal UCHL1 FITC
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应用备注
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The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the CD45RO antibody to be titered up or down for optimal performance.
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. FACS: 0.5-1 μg/million cells,IF: 1-2 μg/mL,IHC (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1),Prediluted format : incubate for 30 min at RT (2) -
限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- 1 mg/mL in 1X PBS, BSA free, sodium azide free
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储存液
- Azide free
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储存条件
- 4 °C,-20 °C
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储存方法
- Store the CD45RO antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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- CD45RO
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别名
- CD45RO
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背景
- This antibody recognizes a 180-185 kDa protein, identified as isoform of leukocyte common antigen (CD45) called CD45RO (4th Leucocyte Typing Workshop: Code No. N31). The epitope recognized by this antibody is sensitive to neuraminidase digestion. CD45RO is found on mature activated T-cells, most thymocytes, and a sub-population of resting T-cells within both CD4 and CD8 subsets. The CD45RO antibody shows no reactivity with normal B or natural killer cells, but reacts with granulocytes and monocytes. Reportedly, antibody to CD45RO is useful to identify T-cell lymphomas and leukemias. It rarely stains NK cells or B-cell lymphomas.
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基因ID
- 5788
抗原
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