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MLANA 抗体

MLANA 适用: 人, 小鼠, 大鼠, 犬 WB, IF, FACS, IHC (p) 宿主: 小鼠 Monoclonal A103-M2-7C10-M2-9E3 unconjugated
产品编号 ABIN3026331
发货至: 中国
  • 抗原 See all MLANA 抗体
    MLANA (Melan A (MLANA))
    适用
    • 176
    • 102
    • 84
    • 32
    • 15
    人, 小鼠, 大鼠, 犬
    宿主
    • 90
    • 85
    • 1
    小鼠
    克隆类型
    • 108
    • 68
    单克隆
    标记
    • 66
    • 15
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    • 5
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    This MLANA antibody is un-conjugated
    应用范围
    • 126
    • 96
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    • 78
    • 58
    • 53
    • 26
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    • 22
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    Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    产品特性
    This antibody cocktail recognizes a protein doublet of 20-22 kDa, identified as MART-1 (Melanoma Antigen Recognized by T cells 1) or Melan-A. MART-1 is a melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. There are seven other melanoma associated antigens recognized by autologous cytotoxic T cells: MAGE-1, MAGE-3, tyrosinase, gp100, gp75, BAGE-1, and GAGE-1. Subcellular fractionation shows that MART-1 is present in melanosomes and endoplasmic reticulum. This MART-1 antibody cocktail labels melanomas and other tumors showing melanocytic differentiation. It is also a useful positive-marker for angiomyolipomas. The antibody cocktail does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin.
    纯化方法
    Protein G affinity chromatography
    免疫原
    Recombinant human protein (A103, M2-7C10, M2-9E3) was used as the immunogen for this MART-1 antibody cocktail.
    克隆位点
    A103-M2-7C10-M2-9E3
    亚型
    IgG1
    Top Product
    Discover our top product MLANA Primary Antibody
  • 应用备注
    The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.

    1. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10  mM Citrate Buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
    2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. FACS: 0.5-1 μg/million cells,IF: 0.5-1 μg/mL,WB: 0.5-1.0 μg/mL,IHC (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1),Prediluted format : incubate for 30 min at RT (2)

    限制
    仅限研究用
  • 浓度
    1 mg/mL
    缓冲液
    1 mg/mL in 1X PBS, BSA free, sodium azide free
    储存液
    Azide free
    储存条件
    4 °C,-20 °C
    储存方法
    Store the MART-1 antibody cocktail at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
  • 抗原
    MLANA (Melan A (MLANA))
    别名
    MART-1 (MLANA 产品)
    别名
    A930034P04Rik antibody, Mart1 antibody, MART-1 antibody, MART1 antibody, melan-A antibody, Mlana antibody, MLANA antibody
    背景
    This antibody cocktail recognizes a protein doublet of 20-22  kDa, identified as MART-1 (Melanoma Antigen Recognized by T cells 1) or Melan-A. MART-1 is a melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. There are seven other melanoma associated antigens recognized by autologous cytotoxic T cells: MAGE-1, MAGE-3, tyrosinase, gp100, gp75, BAGE-1, and GAGE-1. Subcellular fractionation shows that MART-1 is present in melanosomes and endoplasmic reticulum. This MART-1 antibody cocktail labels melanomas and other tumors showing melanocytic differentiation. It is also a useful positive-marker for angiomyolipomas. The antibody cocktail does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin.
    基因ID
    2315
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