CD68 抗体
Quick Overview for CD68 抗体 (ABIN3026325)
抗原
See all CD68 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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产品特性
- This antibody cocktail recognizes a glycoprotein of 110 kDa, which is identified as CD68. antibody to CD68 is important for identifying macrophages in tissue sections. It stains macrophages in a wide variety of human tissues, including Kupffer cells and macrophages in the red pulp of the spleen, in lamina propria of the gut, in lung alveoli, and in bone marrow. CD68 antibody reacts with myeloid precursors and peripheral blood granulocytes. It also reacts with plasmacytoid T cells, which are supposed to be of monocyte/macrophage origin. CD68 shows strong granular cytoplasmic staining of chronic and acute myeloid leukemia and also reacts with rare cases of true histiocytic neoplasia. Lymphomas are negative or show few granules.
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纯化方法
- Protein G affinity chromatography
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免疫原
- The subcellular fraction of human alveolar macrophages was used as the immunogen for this CD68 antibody.
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亚型
- IgG1 kappa
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anti-CD68 Molecule (CD68) (AA 312-326), (Middle Region) antibody
CD68 适用: 小鼠, 大鼠 FACS, IHC, WB, IF 宿主: 兔 Polyclonal unconjugated
anti-CD68 Molecule (CD68) (AA 42-155) antibodyCD68 适用: 人 FACS, IHC, WB, ELISA, ICC 宿主: 小鼠 Monoclonal 3F7D3 unconjugated
anti-CD68 Molecule (CD68) antibodyCD68 适用: 人, 小鼠, 猴, 大鼠 FACS, IHC, WB, IF, ISt, StM 宿主: 小鼠 Monoclonal C68-684 unconjugated
anti-CD68 Molecule (CD68) (AA 21-120) antibodyCD68 适用: 人, 小鼠, 大鼠 ELISA, IHC (p), ICC, IF (p), IF (cc) 宿主: 兔 Polyclonal unconjugated
anti-CD68 Molecule (CD68) (AA 150-301) antibodyCD68 适用: 人 FACS, IHC, ELISA, ISt, StM, Coat 宿主: 小鼠 Monoclonal LAMP4-1830 unconjugated
anti-CD68 Molecule (CD68) antibodyCD68 适用: 人 FACS, IHC, WB, IF, ISt, StM 宿主: 小鼠 Monoclonal rLAMP4-824 unconjugated Recombinant Antibody
anti-CD68 Molecule (CD68) (AA 150-221) antibodyCD68 适用: 人 IHC, StM 宿主: 小鼠 Monoclonal C68-2709 unconjugated
anti-CD68 Molecule (CD68) antibody (PE)CD68 适用: 人 FACS 宿主: 小鼠 Monoclonal Y1-82A PE
anti-CD68 Molecule (CD68) (AA 22-319) antibodyCD68 适用: 人 IHC, WB, IF 宿主: 兔 Polyclonal unconjugated
anti-CD68 Molecule (CD68) (AA 341-354), (C-Term) antibodyCD68 适用: 人 IHC, WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the CD68 antibody to be titered up or down for optimal performance.
1. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. FACS: use antibody at 0.5-1 μg/million cells,IF: 0.5-1 μg/mL,IHC (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1),Prediluted format : incubate for 30 min at RT (2) -
限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- 1 mg/mL in 1X PBS, BSA free, sodium azide free
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储存液
- Azide free
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储存条件
- 4 °C,-20 °C
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储存方法
- Store the CD68 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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- CD68 (CD68 Molecule (CD68))
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别名
- CD68
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背景
- This antibody cocktail recognizes a glycoprotein of 110 kDa, which is identified as CD68. antibody to CD68 is important for identifying macrophages in tissue sections. It stains macrophages in a wide variety of human tissues, including Kupffer cells and macrophages in the red pulp of the spleen, in lamina propria of the gut, in lung alveoli, and in bone marrow. CD68 antibody reacts with myeloid precursors and peripheral blood granulocytes. It also reacts with plasmacytoid T cells, which are supposed to be of monocyte/macrophage origin. CD68 shows strong granular cytoplasmic staining of chronic and acute myeloid leukemia and also reacts with rare cases of true histiocytic neoplasia. Lymphomas are negative or show few granules.
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基因ID
- 968
抗原
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