Helicobacter Pylori 抗体
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- 抗原 See all Helicobacter Pylori (H. pylori) products
- Helicobacter Pylori (H. pylori)
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适用
- Helicobacter pylori
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This Helicobacter Pylori antibody is un-conjugated
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应用范围
- Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS)
- 纯化方法
- Protein A affinity chromatography
- 免疫原
- Total sonicate of Helicobacter ylori was used as the immunogen for this antibody.
- 亚型
- IgG
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- 应用备注
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The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
\. FACS: 0.5-1 μg/million cells,IF: 1-2 μg/mL,IHC (FFPE): 1-2 μg/mL for 30 minutes at RT (1) - 限制
- 仅限研究用
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- 浓度
- 1 mg/mL
- 缓冲液
- 1mg/ml in 1X PBS with 0.05% sodium azide; BSA-free
- 储存液
- Azide free
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store the Helicobacter Pylori antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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- 抗原
- Helicobacter Pylori (H. pylori)
- 别名
- Helicobacter Pylori (H. pylori 产品)
- 物质类
- Virus
- 背景
- The spiral shaped bacterium Helicobacter pylori is strongly associated with inflammation of the stomach and is also implicated in the development of gastric malignancy. This bacteria is known to cause peptic ulcers and chronic gastritis in human. It is associated with duodenal ulcers and may be involved in development of adenocarcinoma and low-grade lymphoma of mucosa associated lymphoid tissue in the stomach. This antibody stains the individual Helicobacter pylori bacterium when it presents on the surface of the epithelium or in the cytoplasm of the epithelial cells in biopsy tissue sections from the antrum and body of the stomach.
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