FOXP3 抗体 (N-Term)
Quick Overview for FOXP3 抗体 (N-Term) (ABIN3026198)
抗原
See all FOXP3 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- N-Term
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纯化方法
- Protein G affinity chromatography
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免疫原
- An N-terminal recombinant protein fragment was used as the immunogen for this FOXP3 antibody.
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亚型
- IgG1 kappa
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应用备注
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Titering of the FOXP3 antibody may be required for optimal performance.
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris buffer with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. FACS: 0.5-1 μg/million cells,IF: 0.5-1 μg/mL,IHC (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1),Prediluted format : incubate for 30 min at RT (2) -
限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- 1 mg/mL in 1X PBS, BSA free, sodium azide free
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储存液
- Azide free
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储存条件
- 4 °C,-20 °C
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储存方法
- Store the FOXP3 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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- FOXP3 (Forkhead Box P3 (FOXP3))
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别名
- FOXP3
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背景
- The FOX family of transcription factors is a large group of proteins that share a common DNA binding domain termed a winged-helix or forkhead domain. FOXP3 is essential for normal immune homeostasis. It represses transcription through a DNA binding forkhead domain, thereby regulating T cell activation.
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基因ID
- 50943
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途径
- Chromatin Binding, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation
抗原
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