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FOXP3 抗体 (N-Term)

This anti-FOXP3 antibody is a 小鼠 单克隆 antibody detecting FOXP3 in FACS, IF 和 IHC (p). Suitable for 人 和 小鼠.
产品编号 ABIN3026198
发货至: 中国

Quick Overview for FOXP3 抗体 (N-Term) (ABIN3026198)

抗原

See all FOXP3 抗体
FOXP3 (Forkhead Box P3 (FOXP3))

适用

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人, 小鼠

宿主

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小鼠

克隆类型

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单克隆

标记

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This FOXP3 antibody is un-conjugated

应用范围

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Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

克隆位点

FHBP3-1
  • 抗原表位

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    N-Term

    纯化方法

    Protein G affinity chromatography

    免疫原

    An N-terminal recombinant protein fragment was used as the immunogen for this FOXP3 antibody.

    亚型

    IgG1 kappa
  • 应用备注

    Titering of the FOXP3 antibody may be required for optimal performance.

    1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Tris buffer with 1  mM EDTA,  pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes.
    2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. FACS: 0.5-1 μg/million cells,IF: 0.5-1 μg/mL,IHC (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1),Prediluted format : incubate for 30 min at RT (2)

    限制

    仅限研究用
  • 浓度

    1 mg/mL

    缓冲液

    1 mg/mL in 1X PBS, BSA free, sodium azide free

    储存液

    Azide free

    储存条件

    4 °C,-20 °C

    储存方法

    Store the FOXP3 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
  • 抗原

    FOXP3 (Forkhead Box P3 (FOXP3))

    别名

    FOXP3

    背景

    The FOX family of transcription factors is a large group of proteins that share a common DNA binding domain termed a winged-helix or forkhead domain. FOXP3 is essential for normal immune homeostasis. It represses transcription through a DNA binding forkhead domain, thereby regulating T cell activation.

    基因ID

    50943

    途径

    Chromatin Binding, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation
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