CD55 抗体
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北京 101111
Quick Overview for CD55 抗体 (ABIN3026018)
抗原
See all CD55 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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纯化方法
- Protein G affinity chromatography
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免疫原
- PHA stimulated human PBL were used as the immunogen for the CD55 antibody.
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亚型
- IgG1 kappa
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应用备注
- Optimal dilution of the CD55 antibody should be determined by the researcher.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Immunohistochemistry (Frozen): 0.5-1 μg/mL for 30 min at RT
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限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- 1 mg/mL in 1X PBS, BSA free, sodium azide free
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储存液
- Azide free
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储存条件
- 4 °C,-20 °C
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储存方法
- Store the CD55 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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- CD55 (Complement Decay-Accelerating Factor (CD55))
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别名
- CD55 (DAF)
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背景
- Recognizes a single chain glycoprotein of 70 kDa, identified as CD55 (also known as decay accelerating factor, DAF). CD55/DAF is widely expressed on cells throughout the body including leukocytes, erythrocytes, epithelium, endothelium, and fibroblasts. It is a Glycosyl phosphatidylinositol anchored (GPI-anchored) member of the membrane bound complement regulatory proteins that inhibit autologous complement cascade activation. It prevents the amplification steps of the complement cascade by interfering with the assembly of the C3-convertases, C4b2a and C3bBb, and the C5-convertase, C4b2a3b and C3bBb3b. CD55 also serves as receptor for CD97 and for echovirus and Coxsackie B virus. The mAb 143-30 can be used as marker for paroxysmal nocturnal hemoglobinuria (PNH).
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途径
- Complement System, Regulation of Leukocyte Mediated Immunity
抗原
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