CD99 抗体
Quick Overview for CD99 抗体 (ABIN3025995)
抗原
See all CD99 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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纯度
- Unpurified high titer supernatant
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免疫原
- Human acute lymphocytic leukemia T-cells (12E7) and recombinant human protein (MIC2/877) was used as the immunogen for the CD99 antibody cocktail.
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亚型
- IgG
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anti-CD99 (CD99) (AA 1-185) antibody
CD99 适用: 人 FACS, WB, IHC, ELISA, ICC 宿主: 小鼠 Monoclonal 7E11C4 unconjugated
anti-CD99 (CD99) (AA 1-185) antibodyCD99 适用: 人, 小鼠 FACS, WB, IHC, ELISA 宿主: 小鼠 Monoclonal 1B10E12 unconjugated
anti-CD99 (CD99) (AA 23-122) antibodyCD99 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 3A10 unconjugated
anti-CD99 (CD99) (AA 23-185) antibodyCD99 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-CD99 (CD99) (AA 27-161) antibodyCD99 适用: 小鼠 FACS, WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-CD99 (CD99) (AA 26-160) antibodyCD99 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-CD99 (CD99) antibodyCD99 适用: 人 FACS, MS 宿主: 小鼠 Monoclonal 3B2-TA8 unconjugated
anti-CD99 (CD99) antibodyCD99 适用: 人 WB, IHC, IHC (p) 宿主: 小鼠 Monoclonal 5C8 unconjugated
anti-CD99 (CD99) antibody (PE)CD99 适用: 人 FACS 宿主: 小鼠 Monoclonal 3B2-TA8 PE
anti-CD99 (CD99) antibodyCD99 适用: 人 FACS, WB, IHC, ELISA 宿主: 兔 Monoclonal 9A5 unconjugated Recombinant Antibody
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应用备注
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Optimal dilution of the CD99 antibody cocktail should be determined by the researcher.
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 5-10 μL/million cells in 0.1ml,Immunofluorescence: 1:100-1:200,Immunohistochemistry (FFPE): 1:100-1:200 for 30 min at RT (1),Prediluted format : incubate for 30 min at RT (2) -
限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- Bioreactor concentrate with 0.05 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C,4 °C
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储存方法
- Store the CD99 antibody c°Cktail at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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- CD99
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别名
- CD99
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背景
- Recognizes a sialoglycoprotein of 27-32 kDa, identified as CD99, or MIC2 gene product, or E2 antigen. MIC2 gene is located in the pseudo-autosomal region of the human X and Y chromosome. MIC2 gene encodes two distinct proteins, which are produced by alternative splicing of the CD99 gene transcript and are identified as bands of 30 and 32 kDa (p30/32). Although its function is not fully understood, CD99 is implicated in various cellular processes including homotypic aggregation of T cells, upregulation of T cell receptor and MHS molecules, apoptosis of immature thymocytes and leukocyte diapedesis. CD99 is expressed on the cell membrane of some lymphocytes, cortical thymocytes, and granulosa cells of the ovary. Most pancreatic islet cells, Sertoli cells of the testis, and some endothelial cells express this antigen. Mature granulocytes express very little or no CD99. MIC2 is strongly expressed on Ewing's sarcoma cells and primitive peripheral neuroectodermal tumors.
抗原
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