GRP94 抗体
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北京 101111
Quick Overview for GRP94 抗体 (ABIN3025969)
抗原
See all GRP94 (HSP90B1) 抗体适用
宿主
克隆类型
标记
应用范围
质量等级
克隆位点
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原理
- GRP94 Antibody / HSP90B1 (azide and preservative free)
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纯化方法
- Protein G affinity chromatography
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免疫原
- Purified glucose regulated protein 94 from chicken oviducts was used as the immunogen for the GRP94 antibody.
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亚型
- IgG2a, kappa
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应用备注
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Optimal dilution of the GRP94 antibody should be determined by the researcher.
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- 1 mg/mL in 1X PBS, BSA free, sodium azide free
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储存液
- Azide free
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储存条件
- 4 °C,-20 °C
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储存方法
- Store the GRP94 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
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- GRP94 (HSP90B1) (Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1))
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别名
- GRP94
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背景
- Recognizes a protein of 94 kDa, which is identified as the glucose-regulated protein 94 (grp94) and also tumor rejection antigen (gp96). Grp94 shows a high degree of sequence homology with the heat shock protein 90 (hsp90). This mAb is highly specific to grp94 and shows minimal cross-reaction with other members of the HSP90 family. Grps are a class of proteins unresponsive to heat shock and are induced by glucose deprivation. Grp94 has been briefly studied as a prognostic factor in breast cancer.
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UniProt
- P14625
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途径
- Thyroid Hormone Synthesis, Activation of Innate immune Response, ER-Nucleus Signaling, Toll-Like Receptors Cascades
抗原
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