NKX2-1 抗体
Quick Overview for NKX2-1 抗体 (ABIN3025852)
抗原
See all NKX2-1 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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纯化方法
- Protein G affinity chromatography
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免疫原
- Full length rat recombinant protein was used as the immunogen for this NKX2.1 antibody.
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亚型
- IgG1 kappa
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anti-NK2 Homeobox 1 (NKX2-1) antibody
NKX2-1 适用: 人 WB, IHC, IF 宿主: 兔 Monoclonal unconjugated
anti-NK2 Homeobox 1 (NKX2-1) antibodyNKX2-1 适用: 人 WB, IHC, FACS, ELISA 宿主: 兔 Monoclonal 6C11 unconjugated Recombinant Antibody
anti-NK2 Homeobox 1 (NKX2-1) antibodyNKX2-1 适用: 人, 小鼠, 大鼠 IHC, StM 宿主: 兔 Monoclonal NX2-1-1855R unconjugated Recombinant Antibody
anti-NK2 Homeobox 1 (NKX2-1) antibodyNKX2-1 适用: 人, 小鼠, 大鼠 IHC, StM 宿主: 小鼠 Monoclonal rNX2-1-690 unconjugated Recombinant Antibody
anti-NK2 Homeobox 1 (NKX2-1) antibodyNKX2-1 适用: 人, 小鼠, 大鼠 IHC, FACS, IF, StM 宿主: 小鼠 Monoclonal 8G7G3-1 unconjugated
anti-NK2 Homeobox 1 (NKX2-1) antibodyNKX2-1 适用: 人 IHC 宿主: 兔 Monoclonal MSVA-312R unconjugated Recombinant Antibody
anti-NK2 Homeobox 1 (NKX2-1) (AA 1-30), (N-Term) antibodyNKX2-1 适用: 人, 小鼠 WB, FACS 宿主: 兔 Polyclonal RB28559 unconjugated
anti-NK2 Homeobox 1 (NKX2-1) antibodyNKX2-1 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-NK2 Homeobox 1 (NKX2-1) (AA 77-158) antibodyNKX2-1 适用: 人 WB, IF, ELISA 宿主: 小鼠 Monoclonal 2B9 unconjugated
anti-NK2 Homeobox 1 (NKX2-1) (N-Term) antibodyNKX2-1 适用: 人 IHC, IHC (p), HIER 宿主: 小鼠 Monoclonal SPT24 unconjugated
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应用备注
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The optimal dilution of the NKX2.1 antibody for each application should be determined by the researcher.
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1),Prediluted format: incubate for 30 min at RT (2) -
限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- 1 mg/mL in 1X PBS, BSA free, sodium azide free
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储存液
- Azide free
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储存条件
- 4 °C,-20 °C
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储存方法
- Store the NKX2.1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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- NKX2-1 (NK2 Homeobox 1 (NKX2-1))
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别名
- NKX2.1
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背景
- Recognizes a protein of 40 kDa, identified as NKX2.1 or Thyroid transcription factor-1 (TTF-1). TTF-1 is a member of the NKx2 family of homeodomain transcription factors. It is expressed in epithelial cells of the thyroid gland and the lung. Nuclei from liver, stomach, pancreas, small intestine, colon, kidney, breast, skin, testes, pituitary, prostate, and adrenal glands are unreactive. Anti-NKX2.1 is useful in differentiating primary adenocarcinoma of the lung from metastatic carcinomas originating in the breast, mediastinal germ cell tumors, and malignant mesothelioma. It can also be used to differentiate small cell lung carcinoma from lymphoid infiltrates. Loss of NKX2.1 expression in non-small cell lung carcinoma has been associated with aggressive behavior of such neoplasms. Reactivity is also seen in thyroid malignancies.
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途径
- Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Systemic Arterial Blood Pressure by Hormones, Cellular Glucan Metabolic Process, Feeding Behaviour
抗原
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